CAJ | 학술논문

目的观察黄体酮对大脑中动脉栓塞(MCAO)大鼠脑部炎症反应的抑制作用以及对血脑屏障结构的影响. 方法健康雄性SD大鼠按照完全随机数字表法分为4组,即假手术组、缺血组、溶剂治疗组和黄体酮治疗组.后三组采用线栓法制作MCAO大鼠模型,假手术组只分离、暴露血管,不结扎动脉,不插入尼龙鱼线;黄体酮治疗组造模成功后腹腔注射黄体酮,溶剂治疗组给予等体积的环糊精溶液治疗.Western blot检测梗死半球脑组织内TNF-α与血脑屏障紧密连接蛋白主要成分Claudin5的表达情况.并通过干湿重法检测脑组织含水量的变化. 结果脑梗死发生后,脑部TNF-α咀的表达水平明显升高,Claudin5蛋白的表达水平明显下降.黄体酮治疗后,梗死半球脑组织内TNF-α的表达水平明显下降,Claudin5蛋白的丢失减少,脑组织含水量亦下降. 结论黄体酮可以通过抑制脑梗死大鼠脑部的炎症反应,减轻血脑屏障的破坏,进而发挥神经保护作用.
목적 관찰황체동대대뇌중동맥전새(MCAO)대서뇌부염증반응적억제작용이급대혈뇌병장결구적영향. 방법건강웅성SD대서안조완전수궤수자표법분위4조,즉가수술조、결혈조、용제치료조화황체동치료조.후삼조채용선전법제작MCAO대서모형,가수술조지분리、폭로혈관,불결찰동맥,불삽입니룡어선;황체동치료조조모성공후복강주사황체동,용제치료조급여등체적적배호정용액치료.Western blot검측경사반구뇌조직내TNF-α여혈뇌병장긴밀련접단백주요성분Claudin5적표체정황.병통과간습중법검측뇌조직함수량적변화. 결과뇌경사발생후,뇌부TNF-α저적표체수평명현승고,Claudin5단백적표체수평명현하강.황체동치료후,경사반구뇌조직내TNF-α적표체수평명현하강,Claudin5단백적주실감소,뇌조직함수량역하강. 결론황체동가이통과억제뇌경사대서뇌부적염증반응,감경혈뇌병장적파배,진이발휘신경보호작용.
Objective To investigate the inhibitory effects of progesterone on the inflammatory response and its influence on the structure of the blood brain barrier (BBB) of rats after cerebral infarction. Methods Progesterone was injected intrapefitoneally following permanent middle cerebral artery occlusion (MCAO) in rats. Western blotting was used to detect the expressions of tumor necrosis factor-alpha (TNF-α) and claudin-5, the major component of tight-junction proteins associated with the BBB. The water content in the injured brain tissue was also examined. Results The expression level of TNF-α increased but claudin-5 expression decreased in the brain tissue of all the rats following MCAO. Progesterone treatment significantly reduced the TNF-α expression in comparison with the vehicle-treated rats, resulting also in ameliorated claudin-5 protein loss and reduced water content in the injured brain tissue. Conclusion Progesterone inhibits the inflammatory response and mitigates the brain damage after cerebral infarction in rats, suggesting the role of this steroid as a neuroprotective agent.