CAJ | 학술논문

目的通过对人脑出血后血肿周围不同区域组织中的P75NTR、TrkA表达的检测,探讨其在脑出血后血肿周围组织细胞凋亡中的作用. 方法采集脑出血血肿清除术患者的脑组织标本,分别运用DNA断裂原位末端标记(TUNEL)法及免疫组化技术检测血肿周围及远隔部位组织中细胞凋亡率与P75NTR、TrkA的表达. 结果相对于远隔部位组织,脑出血后血肿周围组织中的细胞凋亡率与P75NTR的表达水平明显增加(P<0.05),而TrkA的表达水平并没有明显变化(P>0.05).P75NTR的阳性细胞率与TUNEL阳性细胞率呈正相关(r=0.628,p=0.000). 结论脑出血后血肿周围组织中凋亡细胞明显增多,P75NTR介导的细胞凋亡通路可能发挥了重要的作用;TrkA在脑出血发生后并没有增量表达以增加细胞存活,未起到拮抗P75NTR介导的细胞凋亡作用.
목적 통과대인뇌출혈후혈종주위불동구역조직중적P75NTR、TrkA표체적검측,탐토기재뇌출혈후혈종주위조직세포조망중적작용. 방법채집뇌출혈혈종청제술환자적뇌조직표본,분별운용DNA단렬원위말단표기(TUNEL)법급면역조화기술검측혈종주위급원격부위조직중세포조망솔여P75NTR、TrkA적표체. 결과상대우원격부위조직,뇌출혈후혈종주위조직중적세포조망솔여P75NTR적표체수평명현증가(P<0.05),이TrkA적표체수평병몰유명현변화(P>0.05).P75NTR적양성세포솔여TUNEL양성세포솔정정상관(r=0.628,p=0.000). 결론뇌출혈후혈종주위조직중조망세포명현증다,P75NTR개도적세포조망통로가능발휘료중요적작용;TrkA재뇌출혈발생후병몰유증량표체이증가세포존활,미기도길항P75NTR개도적세포조망작용.
Objective To detect the expressions of P75NTR and TrkA in different brain tissues surrounding the hematoma in patients with intracerebral hemorrhage (ICH) and their roles in cell apoptosis following ICH. Methods The brain tissue samples were collected from patients with ICH during hematoma evacuation. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and immunohistochemistry were employed to detect the cell apoptosis and expressions of P75NTR and TrkA in the surrounding and distant tissues of the hematoma, respectively. Results Compared with the distant tissues, the tissues surrounding the hematoma showed significantly increased cell apoptotic rate and P75NTR expression (P<0.05), but the expression level of TrkA remained unchanged (P>0.05). A significant positive correlation was found between P75NTR expression and TUNEL-positive cells (r=0.628, P=0.000). Conclusion Cell apoptosis increases significantly in the tissues surrounding the hematoma after ICH, in which P75NTR-mediated apoptosis pathway may play a key role. TrkA is not upregulated after ICH to antagonize P75NTR-mediated cell apoptosis.