CAJ | 학술논문

目的观察单侧和双侧脑深部电刺激(DBS)伏核核心部对条件性线索和小剂量海洛因诱导大鼠海洛因觅药行为的影响,探讨DBS治疗药物成瘾的单双侧靶点选取问题. 方法采用固定比率程序(FR1)建立大鼠海洛因静脉自身给药模型.模型稳定后,将大鼠按照随机数字表法分成对照组、假手术组、左侧DBS组、右侧DBS组和双侧DBS组(每组6只).各DBS组大鼠行单侧或双侧伏核核心部微电极植入术,术后休息5d,在环境消退期对各DBS组大鼠每天电刺激1h(参数:方波,频率130 Hz,电流强度150 μA,波宽100 μs),持续7d.通过比较各组大鼠的有效鼻触数来反映各组大鼠海洛因觅药行为的差异. 结果在条件性线索和小剂量海洛因诱导的复吸测评中,各组大鼠有效鼻触反应数之间的差异具有统计学意义(P＜0.05)；右侧DBS组和双侧DBS组大鼠有效鼻触反应数分别与对照组、假手术组和左侧DBS组相比差异具有统计学意义(P＜0.05)；且右侧DBS组大鼠与双侧DBS组有效鼻触反应数之间的差异无统计学意义(P＞0.05). 结论高频电刺激右侧或双侧伏核核心部均能明显抑制条件性线索和小剂量海洛因诱导的大鼠觅药行为；且右侧DBS可获得与双侧DBS相近的疗效.
목적 관찰단측화쌍측뇌심부전자격(DBS)복핵핵심부대조건성선색화소제량해락인유도대서해락인멱약행위적영향,탐토DBS치료약물성은적단쌍측파점선취문제. 방법 채용고정비솔정서(FR1)건립대서해락인정맥자신급약모형.모형은정후,장대서안조수궤수자표법분성대조조、가수술조、좌측DBS조、우측DBS조화쌍측DBS조(매조6지).각DBS조대서행단측혹쌍측복핵핵심부미전겁식입술,술후휴식5d,재배경소퇴기대각DBS조대서매천전자격1h(삼수:방파,빈솔130 Hz,전류강도150 μA,파관100 μs),지속7d.통과비교각조대서적유효비촉수래반영각조대서해락인멱약행위적차이. 결과 재조건성선색화소제량해락인유도적복흡측평중,각조대서유효비촉반응수지간적차이구유통계학의의(P＜0.05)；우측DBS조화쌍측DBS조대서유효비촉반응수분별여대조조、가수술조화좌측DBS조상비차이구유통계학의의(P＜0.05)；차우측DBS조대서여쌍측DBS조유효비촉반응수지간적차이무통계학의의(P＞0.05). 결론 고빈전자격우측혹쌍측복핵핵심부균능명현억제조건성선색화소제량해락인유도적대서멱약행위；차우측DBS가획득여쌍측DBS상근적료효.
Objective To investigate the effects of unilateral or bilateral deep brain stimulation (DBS) of nucleus accumbens (NAc) on heroin-seeking behaviors in self-administration rats elicited by conditioned cues or small dose of heroin.Methods Self-administration rat models of relapsing in heroin were established with fixed ration program (FR1),and then,the rats were randomly divided into 5 groups:control group,sham stimulation group,left DBS group,right DBS group and bilateral DBS group.The concentric bipolar microelectrodes were implanted unilaterally/bilaterally into the core of NAc in rats of DBS groups.After 5 days recovery,the rats were given a 1-hour-long high frequency electrical stimulation (square pulse and frequency:130 Hz; current intensity:150 μA; pulse width:100 μs) for 7 days during the extinction phase.Heroin-seeking behaviors were elicited by conditioned cues or small dose of heroin,and their differences between groups were compared through the times of active nose-poke responses.Results In the reinstatement induced by conditioned cues and small dose of heroin,significant differences on the active nose-poke responses of rats were noted between each two groups (P＜0.05); both the right DBS group and bilateral DBS group showed significant differences of active nose-poke responses as compared with the control group,the sham stimulation group and the left DBS group (P＜0.05),while no significant difference was obsvred between the right DBS group and the bilateral DBS group (P＞0.05).Conclusion The right or bilateral DBS of NAc core can significantly reduce the heroin-seeking behaviors elicited by conditioned cues and small dose of heroin in self-administration rats,and the right DBS of NAc can obtain almost the same effects of bilateral DBS.