CAJ | 학술논문

目的探讨切口蛋白受体3(notch3)在非功能性垂体腺瘤发病机制中的作用. 方法收集无锡市第二人民医院神经外科自2005年1月至2011年12月收治的16例被病理证实为非功能性垂体腺瘤的肿瘤标本,以及5例正常垂体组织(通过通过组织捐赠获得),采用逆转录-聚合酶链反应、免疫印迹法、免疫组化等方法观察notch3 mRNA及蛋白在非功能性垂体腺瘤和正常垂体组织中的表达情况. 结果在非功能性垂体腺瘤组织中,notch3mRNA及蛋白表达均显著上调,高于正常垂体组织,差异有统计学意义(P＜0.05). 结论 Notch3蛋白的过度表达可能在非功能性垂体腺瘤的生长过程中具有重要意义.
목적 탐토절구단백수체3(notch3)재비공능성수체선류발병궤제중적작용. 방법 수집무석시제이인민의원신경외과자2005년1월지2011년12월수치적16례피병리증실위비공능성수체선류적종류표본,이급5례정상수체조직(통과통과조직연증획득),채용역전록-취합매련반응、면역인적법、면역조화등방법관찰notch3 mRNA급단백재비공능성수체선류화정상수체조직중적표체정황. 결과 재비공능성수체선류조직중,notch3mRNA급단백표체균현저상조,고우정상수체조직,차이유통계학의의(P＜0.05). 결론 Notch3단백적과도표체가능재비공능성수체선류적생장과정중구유중요의의.
Objective To explore the role of Notch3 in the progression of non-functional pituitary adenomas.Methods Sixteen tumor tissue specimens from patients with non-functional pituitary tumors,collected in our hospital from January 2005 to December 2011,and 5 normal human pituitary tissue specimens,donated by the Organization,were obtained.Reverse transcriptase-polymerase chain reaction (RT-PCR),Western blotting and immunohistochemical staining were used to detect the mRNA and protein expressions of Notch3 in the above tissues.Results Notch3 mRNA expression was significantly up-regulated in clinically non-functional pituitary adenomas and Notch3 protein was expressed at an obviously higher level in non-functional pituitary adenomas as compared with those in normal human pituitary tissues (P＜0.05).Conclusion The over-expression of protein Notch3 may facilitate the growth of non-functional pituitary adenomas.