CAJ | 학술논문

目的观察二十烷酸对大鼠原代皮质神经元β-淀粉样前体蛋白(APP)及β-分泌酶(BACE1)表达的影响,探讨饱和脂肪酸在阿尔茨海默病发病机制中的作用. 方法将原代培养的大鼠皮质神经元分为2组:对照组、二十烷酸组.Western blotting法检测神经元APP、BACE1蛋白表达,RT-PCR法分析APP及BACE1 mRNA表达. 结果与对照组相比,二十烷酸组神经元APP751+770mRNA表达和APP蛋白表达显著升高,BACE1蛋白表达也显著升高,差异有统计学意义(P＜0.05). 结论饱和脂肪酸增加APP及BACE1表达,可能在阿尔茨海默病发病机制中起重要作用.
목적 관찰이십완산대대서원대피질신경원β-정분양전체단백(APP)급β-분비매(BACE1)표체적영향,탐토포화지방산재아이자해묵병발병궤제중적작용. 방법 장원대배양적대서피질신경원분위2조:대조조、이십완산조.Western blotting법검측신경원APP、BACE1단백표체,RT-PCR법분석APP급BACE1 mRNA표체. 결과 여대조조상비,이십완산조신경원APP751+770mRNA표체화APP단백표체현저승고,BACE1단백표체야현저승고,차이유통계학의의(P＜0.05). 결론 포화지방산증가APP급BACE1표체,가능재아이자해묵병발병궤제중기중요작용.
Objective To investigate the effect ofeicosanoic acid (EA) on β-amyloid precursor protein (APP) and β-site APP cleaving enzyme 1 (BACE1) expressions in cortical neurons of primary cultured rats.Methods Cortical neurons of primary cultured rats were divided randomly into EA treatment group (being administered with 40 μmol/L eicosanoic acid for 24 h) and control group (medium containing the same amount of solvent).The APP and BACE1 protein levels were detected by Westem blotting.The APP and BACE1 mRNA levels were detected by reverse transcription-polymerase chain reaction (RT-PCR).Results The levels of APP751+770 mRNA,APP protein and BACE1 protein in the EA treatment group were significantly higher than those in control group (P＜0.05).Conclusion The saturated fatty acids increase the APP and BACE1 expressions,which may play an important role in the pathogenesis of Alzheimer's disease.