CAJ | 학술논문

目的探讨鞘内注射、静脉注射与颅内注射3种不同的血管内皮生长因子(VEGF)给药途径对局灶性脑缺血再灌注大鼠侧支循环形成的影响. 方法健康雄性SD大鼠60只采用随机数字表法分为鞘内注射组、颅内注射组、静脉注射组、手术组和假手术组,每组12只.前4组大鼠采用改进的线栓法制作局灶性脑缺血再灌注损伤模型,其中前3组大鼠造模后即刻、24 h、48 h分别于鞘内、颅内、静脉注射10 ng VEGF.造模后12h、3d对大鼠进行神经功能评分,模型后3、7、14d免疫组化染色检测各组大鼠脑梗死半影区血管内皮细胞VEGF及F8因子的表达. 结果免疫组化染色显示鞘内注射组、颅内注射组、静脉注射组、手术组大鼠脑梗死半影区VEGF在再灌注后3d、7d、14d依次增多,差异有统计学意义(P＜0.05)；造模后3、7、14d鞘内注射组、颅内注射组大鼠血管内皮细胞VEGF表达水平高于静脉注射组、手术组,而静脉注射组高于手术组,差异均有统计学意义(P＜0.05).鞘内注射组、颅内注射组、静脉注射、手术组大鼠梗死半影区F8因子在再灌注后3d、7d、14d依次增多,差异有统计学意义(P＜0.05)；造模后3d、7d鞘内注射、颅内注射组、静脉注射组、手术组大鼠脑梗死半影区F8因子的表达差异无统计学意义(P＞0.05)；造模后14d鞘内注射组、颅内注射组大鼠F8因子的表达高于静脉注射组、手术组,而静脉注射组高于手术组,差异有统计学意义(P＜0.05). 结论 3种不同VEGF给药途径都可以促进局灶性脑缺血再灌注大鼠侧支循环形成,但鞘内注射与颅内注射这两种途径的干预效果优于静脉注射.
목적 탐토초내주사、정맥주사여로내주사3충불동적혈관내피생장인자(VEGF)급약도경대국조성뇌결혈재관주대서측지순배형성적영향. 방법 건강웅성SD대서60지채용수궤수자표법분위초내주사조、로내주사조、정맥주사조、수술조화가수술조,매조12지.전4조대서채용개진적선전법제작국조성뇌결혈재관주손상모형,기중전3조대서조모후즉각、24 h、48 h분별우초내、로내、정맥주사10 ng VEGF.조모후12h、3d대대서진행신경공능평분,모형후3、7、14d면역조화염색검측각조대서뇌경사반영구혈관내피세포VEGF급F8인자적표체. 결과 면역조화염색현시초내주사조、로내주사조、정맥주사조、수술조대서뇌경사반영구VEGF재재관주후3d、7d、14d의차증다,차이유통계학의의(P＜0.05)；조모후3、7、14d초내주사조、로내주사조대서혈관내피세포VEGF표체수평고우정맥주사조、수술조,이정맥주사조고우수술조,차이균유통계학의의(P＜0.05).초내주사조、로내주사조、정맥주사、수술조대서경사반영구F8인자재재관주후3d、7d、14d의차증다,차이유통계학의의(P＜0.05)；조모후3d、7d초내주사、로내주사조、정맥주사조、수술조대서뇌경사반영구F8인자적표체차이무통계학의의(P＞0.05)；조모후14d초내주사조、로내주사조대서F8인자적표체고우정맥주사조、수술조,이정맥주사조고우수술조,차이유통계학의의(P＜0.05). 결론 3충불동VEGF급약도경도가이촉진국조성뇌결혈재관주대서측지순배형성,단초내주사여로내주사저량충도경적간예효과우우정맥주사.
Objective To study the collateral circulation in focal cerebral ischemia rats after being injected vascular endothelial growth factor (VEGF) by three different injection ways (intrathecal injection,intracranincal injection and intravenous injection).Methods Sixty healthy male SD rats were randomly divided into five groups:intrathecal injection (IT) group,intracranincal injection (IC) group,intravenous injection (IV) group,operation (OP) group and sham operated (SH) group (n=12).Middle cerebral artery occlusion (MCAO) was performed to induce focal cerebral ischemia models in rats of the four groups,except the sham-operated group; 10 ng VEGF was given to the injection groups right after the inducement,24 and 48 h after the inducement.The neurological scale was performed in all the rats 12 h and 3 d after the inducement.The expressions of VEGF and F8 in the periphery of infarcts were detected in the five groups 3,7 and 14 d after ischemia reperfusion.Results The VEGF expressions in the four groups,expected the sham-operated group,increased gradually 3,7 and 14 d after ischemia reperfusion,with significant difference between each two time points (P＜0.05).The VEGF expressions of IT and IC groups were higher than that in the IV and OP groups (P＜0.05),and that of IV group was significantly higher than that of OP group (P＜0.05).The F8 expression in the four groups,expected the sham-operated group,increased gradually 3,7 and 14 d after ischemia reperfusion,with significant difference between each two time points (P＜0.05).The F8 expression of IT and IC groups was obviously higher than that of the IV and OP groups 14 d after reperfusion (P＜0.05),and that of IV group was significantly higher than that of OP group (P＜0.05).Conclusion All the three different injection ways can improve the formation of collateral circulation in MACO rats,but intrathecal and intracranincal injections are better than intravenous injection.