CAJ | 학술논문

目的探讨神经生长因子对糖尿病周围神经病变大鼠神经肽和神经传导速度的影响. 方法雄性Wistar大鼠35只按随机数字表法分为健康对照组(n=10)、糖尿病模型组(n=13)和神经生长因子治疗组(n=12),后两组用链脲佐菌素制成糖尿病周围神经病变大鼠模型,并给予神经生长因子治疗组神经生长因子治疗(40μg/kg).显微镜下观察并计算背根神经节中P物质、降钙素基因相关肽(CGRP)免疫阳性细胞率,检测运动神经传导速度(MNCV)和感觉神经传导速度(SNCV). 结果糖尿病模型组大鼠背根神经节中P物质、CGRP免疫阳性细胞率(27.710％±3.471％；36.360％±12.027％)以及神经生长因子治疗组治疗前MNCV [(35.80±6.19) m/s]、SNCV[(39.62±6.69) m/s]与健康对照组[P物质:44.225％±8.213％；CGRP:47.400％±13.723％；MNCV:(55.83±10.30) m/s; SNCV:(47.02±7.52) m/s]相比显著下降,差异有统计学意义(P＜0.05).经神经生长因子治疗后,P物质、CGRP免疫阳性细胞率(49.417％±6.753％;53.811％±7.125％)较糖尿病模型组显著增高,MNCV[(41.80±3.45) m/s]、SNCV[(42.92±6.69) m/s]均治疗前显著增高,差异有统计学意义(P＜0.05). 结论糖尿病周围神经病变大鼠可出现神经传导速度下降和神经生长因子相关神经肽P物质、CGRP缺乏,而神经生长因子可促进神经肽的表达并提高神经传导速度.
목적 탐토신경생장인자대당뇨병주위신경병변대서신경태화신경전도속도적영향. 방법 웅성Wistar대서35지안수궤수자표법분위건강대조조(n=10)、당뇨병모형조(n=13)화신경생장인자치료조(n=12),후량조용련뇨좌균소제성당뇨병주위신경병변대서모형,병급여신경생장인자치료조신경생장인자치료(40μg/kg).현미경하관찰병계산배근신경절중P물질、강개소기인상관태(CGRP)면역양성세포솔,검측운동신경전도속도(MNCV)화감각신경전도속도(SNCV). 결과 당뇨병모형조대서배근신경절중P물질、CGRP면역양성세포솔(27.710％±3.471％；36.360％±12.027％)이급신경생장인자치료조치료전MNCV [(35.80±6.19) m/s]、SNCV[(39.62±6.69) m/s]여건강대조조[P물질:44.225％±8.213％；CGRP:47.400％±13.723％；MNCV:(55.83±10.30) m/s; SNCV:(47.02±7.52) m/s]상비현저하강,차이유통계학의의(P＜0.05).경신경생장인자치료후,P물질、CGRP면역양성세포솔(49.417％±6.753％;53.811％±7.125％)교당뇨병모형조현저증고,MNCV[(41.80±3.45) m/s]、SNCV[(42.92±6.69) m/s]균치료전현저증고,차이유통계학의의(P＜0.05). 결론 당뇨병주위신경병변대서가출현신경전도속도하강화신경생장인자상관신경태P물질、CGRP결핍,이신경생장인자가촉진신경태적표체병제고신경전도속도.
Objective To investigate the influence of nerve growth factor (NGF) in neuropeptides and nerve conduction velocity in rats with experimental diabetic peripheral neuropathy (DNP).Methods Thirty-five 6-week-old male Wistar rats,weighing 175-215 g,were chosen in our study and randomly divided into healthy control group (n=10),DNP model group (n=13) and NGF treatment group (n=12).Rats in the latter 2 group were induced with the interference of administered streptozotocin,and rats in the NGF treatment group were treated with NGF (40 μg/kg).The immunopositive cells percentages of substance P (SP) and calcitonin gene-related peptide (CGRP) in dorsal root ganglion (DRG) were observed and measured; the motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV) were measured and compared among the three groups.Results As compared with those in the healthy control group,the immunopositive cells percentages of SP (27.710％±3.471％ vs 44.225％±8.213％) and CGRP (36.360％±12.027％ vs 47.400％±13.723％) in the DRG of DNP model group were significantly decreased (P＜0.05); as compared with those in the healthy control group,the MNCV[(35.80±6.19) m/s vs (55.83±10.30) m/s] and SNCV[(39.62±6.69) m/s vs (47.02 ±7.52) m/s)] of DNP model group were significantly decreased (P＜0.05); however,obvious increase of SP (49.417％±6.753％) and CGRP (53.811％±7.125％),MNCV (41.80±3.45) m/s and SNCV (42.92±6.69 m/s) in the NGF treatment group was noted as compared with those in the DNP model group (P＜0.05).Conclusion The nerve conduction velocity and NGF related neuropeptides (SP and CGRP) decrease in rats with DNP; treatment with NGF promotes the expression ofneuropeptides and increases the nerve conduction velocity.