CAJ | 학술논문

目的探讨氩氦冷冻治疗9L/Fischer344大鼠脑胶质瘤模型的病理改变. 方法种植9L胶质瘤细胞于30只Fischer344大鼠鼠背建立9L/Fischer344大鼠脑胶质瘤模型,并按随机数字表法分为空白对照组(自然生长,不进行任何处理)和氩氦冷冻组(给予氩氦冷冻治疗).接种后30d取出肿瘤组织进行病理学观察,应用HE染色、电镜下观察冷冻消融的肿瘤组织形态,应用免疫组织化学染色检测胶质纤维酸性蛋白(GFAP)和S-100蛋白表达. 结果 (1)光镜下空白对照组肿瘤细胞巢状排列紧密,肿瘤无包膜,瘤周小血管扩张,可见肿瘤细胞沿小血管浸润生长；瘤内新生血管丰富,瘤内常见出血、凝固性坏死灶.高倍镜下肿瘤细胞形态多样,核大深染、多核,异型性高.肿瘤细胞GFAP、S-100染色阴性,肿瘤细胞间胶质网可见阳性染色.(2)氩氦冷冻组HE染色显示冷冻中央区呈均匀性凝固性坏死,边缘区的炎性反应带主要为一些细胞碎片及大量的红、白细胞浸润:微血管内也发现大量的红细胞、血小板集合和血栓形成,并可见灶性出血.电镜显示冷冻区中央、边缘区组织细胞全部坏死或凋亡. 结论氩氦刀可能是治疗胶质瘤有效的方法.
목적 탐토아양냉동치료9L/Fischer344대서뇌효질류모형적병리개변. 방법 충식9L효질류세포우30지Fischer344대서서배건립9L/Fischer344대서뇌효질류모형,병안수궤수자표법분위공백대조조(자연생장,불진행임하처리)화아양냉동조(급여아양냉동치료).접충후30d취출종류조직진행병이학관찰,응용HE염색、전경하관찰냉동소융적종류조직형태,응용면역조직화학염색검측효질섬유산성단백(GFAP)화S-100단백표체. 결과 (1)광경하공백대조조종류세포소상배렬긴밀,종류무포막,류주소혈관확장,가견종류세포연소혈관침윤생장；류내신생혈관봉부,류내상견출혈、응고성배사조.고배경하종류세포형태다양,핵대심염、다핵,이형성고.종류세포GFAP、S-100염색음성,종류세포간효질망가견양성염색.(2)아양냉동조HE염색현시냉동중앙구정균균성응고성배사,변연구적염성반응대주요위일사세포쇄편급대량적홍、백세포침윤:미혈관내야발현대량적홍세포、혈소판집합화혈전형성,병가견조성출혈.전경현시냉동구중앙、변연구조직세포전부배사혹조망. 결론 아양도가능시치료효질류유효적방법.
Objective To research the pathological features of 9L/Fischer344 of rat gliomas treated by argon helium cryotherapy.Methods The 9L gliomas were planted into 30 Fischer344 rats to establish the 9L/Fischer344 rat models; these rat models were randomly divided into blank control group (not given any treatment) and argon helium cryotherapy group.Thirty d after that,tumor histopathology was observed; HE staining and electron microscopy were employed to observe the morphology of the tumors; immunohistochemical staining was used to detect the GFAP and S-100 protein expressions.Results In the blank control group,light microscope showed gliomas closely nested arrangement without envelope,minute vessels expanding in the surrounding area of the gliomas,and gliomas growing following the minute vessels; abundant neovascularization,hemorrhage and coagulated necrosis in the gloms were noted; At high magnification,morphological diversity,low differentiated degree,big and excessive nucleus with deep staining were noted in the gliomas; GFAP and S-100 preotein was negatively expressed in the gliomas cell,but positive expression was noted in the glial network between gliomas.In the argon helium cryotherapy group,uniformed coagulation necrosis was noted in the central area,and cell debris and a lot of red and leukocyte infiltration were noted in the marginal zone; a large number of red blood cells,platelets and thrombosis,and visible focal hemorrhage were noted in the capillaries;tissue necrosis or cell apoptosis was noted in all central and marginal zones.Conclusion Argon helium cryotherapy might be an effective way for treating gliomas.