CAJ | 학술논문

目的探讨嗅球电刺激对成年大鼠室管膜下区(SVZ)神经前体细胞(NPC)增殖及其向嗅球迁移的影响,并对其相关机制进行初步探讨. 方法 SD大鼠80只按随机数字表法分为正常对照组、假刺激组、电刺激ld组、电刺激3d组、电刺激1周组、电刺激2周组、电刺激3周组、电刺激4周组,每组10只,后6组大鼠制备嗅球电刺激模型,以5-溴脱氧尿嘧啶(Brdu)标记新生细胞,免疫组化染色观察大鼠SVZ区内源性NPC的增殖,RT-PCR检测嗅球内Prokineticin 2 (prk2)mRNA的表达；另取大鼠15只,按随机数字表法分为正常对照组、假刺激组和电刺激4周组,每组5只,免疫组化染色观察嗅球内Brdu阳性细胞的表达. 结果 8组大鼠SVZ区Brdu阳性细胞数不同,差异有统计学意义(F=51.475,P=0.000).与正常对照组和假刺激组比较,电刺激ld、3d、l周、2周组大鼠刺激侧Brdu阳性细胞增高,差异有统计学意义(P＜0.05)；8组大鼠嗅球内prk2 mRNA的表达不同,差异有统计学意义(F=154.067,P=0.000).与正常对照组和假刺激组比较,电刺激ld、3d、l周、2周、3周、4周组prk2 mRNA增加,差异有统计学意义(P＜0.05)；3组大鼠嗅球内Brdu阳性细胞数不同,差异有统计学意义(F=36.472,P=0.000).电刺激4周组嗅球内Brdu阳性细胞数较正常对照组和假刺激组明显增多,差异有统计学意义(P＜0.05). 结论电刺激嗅球可促进SVZ区NPC的增殖并向嗅球迁移,这可能与电刺激提高嗅球内Prk 2的表达水平有关.
목적 탐토후구전자격대성년대서실관막하구(SVZ)신경전체세포(NPC)증식급기향후구천이적영향,병대기상관궤제진행초보탐토. 방법 SD대서80지안수궤수자표법분위정상대조조、가자격조、전자격ld조、전자격3d조、전자격1주조、전자격2주조、전자격3주조、전자격4주조,매조10지,후6조대서제비후구전자격모형,이5-추탈양뇨밀정(Brdu)표기신생세포,면역조화염색관찰대서SVZ구내원성NPC적증식,RT-PCR검측후구내Prokineticin 2 (prk2)mRNA적표체；령취대서15지,안수궤수자표법분위정상대조조、가자격조화전자격4주조,매조5지,면역조화염색관찰후구내Brdu양성세포적표체. 결과 8조대서SVZ구Brdu양성세포수불동,차이유통계학의의(F=51.475,P=0.000).여정상대조조화가자격조비교,전자격ld、3d、l주、2주조대서자격측Brdu양성세포증고,차이유통계학의의(P＜0.05)；8조대서후구내prk2 mRNA적표체불동,차이유통계학의의(F=154.067,P=0.000).여정상대조조화가자격조비교,전자격ld、3d、l주、2주、3주、4주조prk2 mRNA증가,차이유통계학의의(P＜0.05)；3조대서후구내Brdu양성세포수불동,차이유통계학의의(F=36.472,P=0.000).전자격4주조후구내Brdu양성세포수교정상대조조화가자격조명현증다,차이유통계학의의(P＜0.05). 결론 전자격후구가촉진SVZ구NPC적증식병향후구천이,저가능여전자격제고후구내Prk 2적표체수평유관.
Objective To investigate the effects of electrical stimulation of olfactory bulb (OB)on proliferation,migration to OB of neural precursor cells (NPC) in the subventricular zone,and preliminary explore its related mechanism.Methods Eighty adult female Sprague Dawley rats were randomly divided into normal control group,sham stimulation group and stimulation groups of 1 d,3 d,1 week,2 weeks,3 weeks and 4 weeks (n=10).Rats in the later six groups were performed OB electrical stimulation.Five of these rats in each group were injected intraperitoneally with 5-bromo-2-deoxyuridine (Brdu) to mark new-bom cells and immunohistochemical staining was employed to observe the proliferation of NPCs in SVZ; the other 5 were sacrificed at corresponding time to detect the mRNA expression ofprokineticin 2 (prk2) in OB.Another 15 rats were randomly divided into normal control group Ⅰ,sham stimulation group Ⅰ and stimulation group Ⅰ (n=5); four weeks after Brdu was injected,the animals were sacrificed and immumohistochemical staining was used to investigate the number of Brdu-positive cells in OB.Results The number of Brdu-positive cells in SVZ was significantly different among the eight group (F=51.475,P=0.000); as compared with those in the normal control group and sham stimulation group,the Brdu-positive cells were significantly increased in stimulation groups of 1 d,3 d,1 week and 2 weeks (P＜0.05).The mRNA expression ofprk 2 in OB was significantly different among the eight group (F=154.067,P=0.000); as compared with those in the normal control group and sham stimulation group,the Brdu-positive cells were significantly increased in all the stimulation groups (F=36.472,P=0.000).Four weeks after injection of Brdu,the Brdu-positive cells in OB of the stimulation group Ⅰ significantly increased as compared with that in the normal control group Ⅰ and sham stimulation group Ⅰ (P＜0.05).Conclusion Electrical stimulation of OB promotes proliferation,migration of NPCs in SVZ,which may be related to elevated expression of Prk2.