CAJ | 학술논문

目的研究异体神经干细胞大动物脑皮层内定点移植后的存活及分化情况.方法利用大动物(山羊)作为研究对象,通过立体定向方式将用绿色荧光蛋白标记过的异体神经干细胞定点移植到山羊受损脑组织周围皮层内.1个月后处死动物,取移植部位脑组织进行冰冻切片免疫组化染色检测,检测指标为神经系统的3种主要组成细胞(神经元、星形胶质细胞及少突胶质细胞)的特异性标记物——微管相关蛋白2(MAP2)、神经纤维酸性蛋白(GFAP)及少突胶质细胞标志蛋白01(O1).结果移植1个月后,山羊脑皮层内的异体神经干细胞不仅能在宿主体内存活,还能分化为神经系统的3种主要组成细胞——神经元、星形胶质细胞及少突胶质细胞,其中表达MAP2及GFAP的细胞较多.结论异体神经干细胞大动物皮层内移植后可良好存活并分化.
목적 연구이체신경간세포대동물뇌피층내정점이식후적존활급분화정황.방법 이용대동물(산양)작위연구대상,통과입체정향방식장용록색형광단백표기과적이체신경간세포정점이식도산양수손뇌조직주위피층내.1개월후처사동물,취이식부위뇌조직진행빙동절편면역조화염색검측,검측지표위신경계통적3충주요조성세포(신경원、성형효질세포급소돌효질세포)적특이성표기물——미관상관단백2(MAP2)、신경섬유산성단백(GFAP)급소돌효질세포표지단백01(O1).결과 이식1개월후,산양뇌피층내적이체신경간세포불부능재숙주체내존활,환능분화위신경계통적3충주요조성세포——신경원、성형효질세포급소돌효질세포,기중표체MAP2급GFAP적세포교다.결론 이체신경간세포대동물피층내이식후가량호존활병분화.
Objective To research the survival and differentiation of neural stem cells being transplanted into goats' brain cortices.Methods We transplanted the neural stem cells marked by green fluorescence protein (GFP) or saline into the surrounding areas of brain contusion and laceration areas of goat's models by surgical intervention.Then,the survival and differentiation of neural stem cells being transplanted into goats' brain were observed one month later with the method of immtmohistochemistry by detecting the specific markers of neurons,astrocytes and oligodendrocytes:microtubule-associated protein 2 (MAP2),glial fibrillary acidic protein (GFAP) and oligodendrocyte marker protein 01 (O1).Results Neural stem cells survived well in the goats' brain,and differentiated into three types of cells:neurons,astrocytes and oligodendrcytes; and these cells mainly expressed MAP2 and GFAP.Conclusion Neural stem cells can survival,immigrate and different well in the transplanted brains.