CAJ | 학술논문

目的探讨复聪汤对阿尔茨海默病小鼠学习记忆能力的影响及相关机制.方法 20只9月龄APP/PS1双转基因小鼠按照随机数字表法分为2组:复聪汤组灌服药液1 mL/d,共8周；对照组同期给予等量蒸馏水.利用Morris水迷宫检测小鼠空间学习记忆能力,免疫组织化学染色法检测海马CA1区N-甲基-D-门冬氨酸(NMDA)受体2B亚单位(NR2B)蛋白表达的强弱,免疫荧光观察β淀粉样蛋白(Aβ)斑块,电镜观察突触结构.结果 (1)治疗8周后复聪汤组小鼠第1～4天逃避潜伏期较对照组明显缩短,差异均具有统计学意义(P＜0.05).复聪汤组原站台象限停留时间长于对照组,穿越原站台次数多于对照组,差异均具有统计学意义(P＜0.05).(2)治疗后复聪汤组海马CA1区NR2β表达高于对照组,差异具有统计学意义(P＜0.05).(3)复聪汤组Aβ斑块缩小,周围激活的星形胶质细胞减少,而对照组仍以致密型Aβ斑块为主,星形胶质细胞增多.(4)复聪汤组突触结构改善.结论复聪汤可能是通过促使Aβ致密斑向弥散斑转化,使Aβ蛋白沉积导致的神经毒性作用减弱,以及改善突触部位超微结构,促使海马区NR2B蛋白表达增强,来改善AD小鼠的学习记忆能力作用.
목적 탐토복총탕대아이자해묵병소서학습기억능력적영향급상관궤제.방법 20지9월령APP/PS1쌍전기인소서안조수궤수자표법분위2조:복총탕조관복약액1 mL/d,공8주；대조조동기급여등량증류수.이용Morris수미궁검측소서공간학습기억능력,면역조직화학염색법검측해마CA1구N-갑기-D-문동안산(NMDA)수체2B아단위(NR2B)단백표체적강약,면역형광관찰β정분양단백(Aβ)반괴,전경관찰돌촉결구.결과 (1)치료8주후복총탕조소서제1～4천도피잠복기교대조조명현축단,차이균구유통계학의의(P＜0.05).복총탕조원참태상한정류시간장우대조조,천월원참태차수다우대조조,차이균구유통계학의의(P＜0.05).(2)치료후복총탕조해마CA1구NR2β표체고우대조조,차이구유통계학의의(P＜0.05).(3)복총탕조Aβ반괴축소,주위격활적성형효질세포감소,이대조조잉이치밀형Aβ반괴위주,성형효질세포증다.(4)복총탕조돌촉결구개선.결론 복총탕가능시통과촉사Aβ치밀반향미산반전화,사Aβ단백침적도치적신경독성작용감약,이급개선돌촉부위초미결구,촉사해마구NR2B단백표체증강,래개선AD소서적학습기억능력작용.
Objective To investigate the effect of Fucong Tang on learning and memory abilities in A PP/PS1 double transgenic mice with Alzheimer's disease (AD).Methods A total of 20 9-month-old APP/PS1 transgenic mice were randomly divided into two groups:one group receiving Fucong Tang 1 mL/d for 8 weeks (Fucong Tang treatment group) and the other group receiving distilled water treatment.The spatial learning and memory abilities of AD mice were tested with Morris water maze,and Aβ plaques were observed by Aβ+GFAP double immunofluorescence staining,the expression of N-methyl-aspartate (NMDA) receptors subunit 2B (NR2B) by immunohistochemistry,and synapses in CA1 by electron microscopy.Results The 1-4 d escape latency of the Fucong Tang treatment group was significantly shorter than that of the control group (P＜0.005); the time spent in the target quadrants and the frequency of crossing the original platform in the Fucong Tang treatment group were significantly higher than that of the control group (P＜0.05).The average optical density of positive NR2B in the Fucong Tang treatment group was higher that of the control group (P＜0.05).After treatment,Aβ plaques volume and the surrounding activated astrocytes reduced in the Fucong Tang treatment group,while there still presented more compact Aβ plaques and more activated astrocytes in the control group.The damage of synapses of Fucong Tang treatment group presented alleviated.Conclusion Fucong Tang could improve the learning and memory abilities of AD mice by reducing compact Aβ plaques and alleviating associated toxic effects on neurons,and further promoting the expression of NR2B and improving synapse structure.