CAJ | 학술논문

目的观察不同年龄组正常人外周静脉血超氧化物歧化酶1(SOD1)蛋白表达和活性水平,为后续研究神经变性病尤其是肌萎缩侧索硬化(ALS)患者体内的SOD1表达做出前期的方法探索和数据积累.方法随机采集北京协和医院体检中心血库中正常健康人血样120人份(年龄20～78岁)及收集一个ALS家系中目前无ALS临床症状成员血样19份(年龄8个月～19岁),分别收集抗凝的检验用全血约1000 μL和血清约500 μL,其中采用Western blotting方法检测全血中SOD1蛋白的表达,应用黄嘌呤氧化酶法检测血清中SOD活性水平.人员按约10岁一个年龄段进行分组并比较.结果随着年龄的增加,正常人外周静脉血单核细胞中SOD1蛋白表达增加,41～50岁达高峰,60岁后逐渐下降.正常人出生后血清总SOD活性呈下降趋势,20岁之前血清中总SOD活性处于相对高的水平(0.13～0.16 U/mL),20岁后总SOD活性明显下降,随着年龄的增加稍有波动,但一直维持在相对较低的水平(0.07～0.08 U/mL).结论正常人外周血SOD1蛋白和血清总SOD活性随着年龄改变发生变化.
목적 관찰불동년령조정상인외주정맥혈초양화물기화매1(SOD1)단백표체화활성수평,위후속연구신경변성병우기시기위축측색경화(ALS)환자체내적SOD1표체주출전기적방법탐색화수거적루.방법 수궤채집북경협화의원체검중심혈고중정상건강인혈양120인빈(년령20～78세)급수집일개ALS가계중목전무ALS림상증상성원혈양19빈(년령8개월～19세),분별수집항응적검험용전혈약1000 μL화혈청약500 μL,기중채용Western blotting방법검측전혈중SOD1단백적표체,응용황표령양화매법검측혈청중SOD활성수평.인원안약10세일개년령단진행분조병비교.결과 수착년령적증가,정상인외주정맥혈단핵세포중SOD1단백표체증가,41～50세체고봉,60세후축점하강.정상인출생후혈청총SOD활성정하강추세,20세지전혈청중총SOD활성처우상대고적수평(0.13～0.16 U/mL),20세후총SOD활성명현하강,수착년령적증가초유파동,단일직유지재상대교저적수평(0.07～0.08 U/mL).결론 정상인외주혈SOD1단백화혈청총SOD활성수착년령개변발생변화.
Objective To investigate the superoxide dismutase 1 (SOD1) protein expression and its activity in peripheral venous blood of healthy people of different ages to provide basis for further investigating the SOD1 expression in patients with neurodegenerative diseases,amyotrophic lateral sclerosis (ALS) especially.Methods Blood samples were obtained from 19 members without ALS symptom from a family with ALS and 120 healthy people in our hospital.Approximately,1000 μL whole blood and 500 μL serum were collected from each examiner.The SOD1 protein expression was measured by Westem blotting; the SOD1 activity in serum was determined by xanthine oxidase technique; these results were compared between groups being divided according to ages (about 10 ages as a group).Results The SOD1 protein expression in peripheral blood mononuclear cells increased with age,reaching its peak level at age of 40-50 years,and then decreased after 60 years old.The SOD activity in serum showed a decreased trend,but was still at a high level,ranged from 0.13 to 1.16 U/mL; after 20 years old,obvious decreased was noted and maintained a relative low level (0.07-0.08 U/mL).Conclusions The SOD1 protein level in peripheral blood mononuclear cells and SOD activity in serum changed with age in healthy people.