CAJ | 학술논문

目的观察电针对易卒中型肾性高血压大鼠(RHRSP)大脑中动脉闭塞(MCAO)后不同时间点大脑皮层、延髓、颈髓区中枢神经髓鞘相关生长抑制因子Nogo-A mRNA表达的影响,探讨其对急性脑梗死远隔损害的保护机制. 方法应用随机化原则,对SD雄性大鼠采用双肾双夹术复制成RHRSP模型及采取线栓法制作成MCAO模型,共分为高血压组、假手术组、模型组、电针组、假针刺组(每组各60只),其中高血压组为单纯RHRSP模型,假手术组仅做手术创伤不行MCAO,模型组、电针组、假针刺组分别在RHRSP模型和MCAO模型基础上不处理、选取督脉“百会”和“大椎”穴进行电针治疗(1次/d,共28 d)以及将针灸针贴于上述相同穴位处皮肤.治疗第1、7、14、28天分别分离各组相应数量大鼠右侧大脑皮层、延髓和左侧颈髓,采用RT-PCR法检测Nogo-AmRNA的表达. 结果 (1)皮层区:与高血压组比较,治疗第7、14、28天模型组、电针组、假针刺组Nogo-A mRNA表达明显升高,差异有统计学意义(P＜0.05);与模型组比较,治疗第7、14、28天电针组Nogo-A mRNA表达明显降低,差异有统计学意义(P＜0.05).(2)延髓区:与高血压组比较,治疗第14、28天模型组、电针组、假针刺组Nogo-A mRNA表达明显升高,差异有统计学意义(P＜0.05);与模型组比较,治疗第14、28天电针组Nogo-A mRNA表达明显降低,差异有统计学意义(P＜0.05).(3)颈髓区:与高血压组比较,治疗第28天模型组、电针组、假针刺组Nogo-A rnRNA表达明显升高,差异有统计学意义(P＜0.05);与模型组比较,治疗第28天电针组Nogo-A mRNA表达明显降低,差异有统计学意义(P＜0.05). 结论大脑皮层、延髓、颈髓区Nogo-A mRNA表达增高是急性脑梗死远隔损害发生的一个重要原因,电针对高血压性脑梗死大鼠的保护作用可能与其下调Nogo-A mRNA表达密切相关.
목적 관찰전침대역졸중형신성고혈압대서(RHRSP)대뇌중동맥폐새(MCAO)후불동시간점대뇌피층、연수、경수구중추신경수초상관생장억제인자Nogo-A mRNA표체적영향,탐토기대급성뇌경사원격손해적보호궤제. 방법 응용수궤화원칙,대SD웅성대서채용쌍신쌍협술복제성RHRSP모형급채취선전법제작성MCAO모형,공분위고혈압조、가수술조、모형조、전침조、가침자조(매조각60지),기중고혈압조위단순RHRSP모형,가수술조부주수술창상불행MCAO,모형조、전침조、가침자조분별재RHRSP모형화MCAO모형기출상불처리、선취독맥“백회”화“대추”혈진행전침치료(1차/d,공28 d)이급장침구침첩우상술상동혈위처피부.치료제1、7、14、28천분별분리각조상응수량대서우측대뇌피층、연수화좌측경수,채용RT-PCR법검측Nogo-AmRNA적표체. 결과 (1)피층구:여고혈압조비교,치료제7、14、28천모형조、전침조、가침자조Nogo-A mRNA표체명현승고,차이유통계학의의(P＜0.05);여모형조비교,치료제7、14、28천전침조Nogo-A mRNA표체명현강저,차이유통계학의의(P＜0.05).(2)연수구:여고혈압조비교,치료제14、28천모형조、전침조、가침자조Nogo-A mRNA표체명현승고,차이유통계학의의(P＜0.05);여모형조비교,치료제14、28천전침조Nogo-A mRNA표체명현강저,차이유통계학의의(P＜0.05).(3)경수구:여고혈압조비교,치료제28천모형조、전침조、가침자조Nogo-A rnRNA표체명현승고,차이유통계학의의(P＜0.05);여모형조비교,치료제28천전침조Nogo-A mRNA표체명현강저,차이유통계학의의(P＜0.05). 결론 대뇌피층、연수、경수구Nogo-A mRNA표체증고시급성뇌경사원격손해발생적일개중요원인,전침대고혈압성뇌경사대서적보호작용가능여기하조Nogo-A mRNA표체밀절상관.
Objective To observe the effect of electric acupuncture on Nogo-A mRNA expression in the cortex,medulla oblongata and cervical cord areas of models of stroke-prone renovascular hypertensive rats (RHRSP) after middle cerebral artery occlusion (MCAO) at different time points,and investigate the possible mechanisms of electric acupuncture in diaphragma damage in ischemic stroke.Methods Male SD rats were divided into hypertension group,sham-operated group,model group,electric acupuncture group and sham electric acupuncture group by random number table method (n=60).Rats of hypertension group were the pure RHRSP models; rats of sham-operated group only received surgical trauma; rats of model group received no treatment based RHRSP and MCAO; "Baihui" and "Dazhui" acupoints were electroacupuncted in rats of electric acupuncture group for 28 days,once a day; in sham electric acupuncture group,acupuncture needles were sticked on the skin of rats where "Baihui" and "Dazhui" locate.One,7,14,28 days after treatment,the right side of the cortex,medulla oblongata and the left side of the cervical cord were separated in a certain number of each group rats.RT-PCR was employed to detect the Nogo-A mRNA expression.Results (1) Cortex area:on the 7th,14th,28th days,model group,electric acupuncture group and sham electric acupuncture group had higher Nogo-A mRNA expression than hypertension group with statistically significant difference (P＜ 0.05); as compared with the model group,electric acupuncture group had statistically lower Nogo-A mRNA expression on the 7th,14th,28th days (P＜0.05).(2) Medulla oblongata area:on the 14th and 28th days,model group,electric acupuncture group and sham electric acupuncture group had higher Nogo-A mRNA expression than hypertension group with statistically significant difference (P＜0.05); as compared with the model group,electric acupuncture group had statistically lower Nogo-A mRNA expression on the 14th and 28th days (P＜0.05).(3) Cervical cord area:on the 28th day,model group,electric acupuncture group and sham electric acupuncture group had higher Nogo-A mRNA expression than hypertension group with statistically significant difference (P＜0.05); as compared with model group,electric acupuncture group had statistically lower Nogo-A mRNA expression on the 28th day (P＜0.05).Conclusions The increase ofNogo-A rnRNA expression in the cerebral cortex,medulla oblongata and cervicall cord may be an important factor which involves in acute cerebral ischemic diaphragma damage.Electric acupuncture's protection to central nervous of cerebral ischemic RHRSP may closely relate to decreasing Nogo-A mRNA expression.