CAJ | 학술논문

目的观察猴脑边缘区甲硫氨酸-脑啡肽(MET-ENK)阳性免疫反应神经终末突触的超微结构,为进一步探索其学习记忆功能提供超微结构基础. 方法选取健康成年雄性恒河猴6只,常规灌注固定和取脑,冰冻切片后行免疫组织化学染色检测纹状体MET-ENK的表达,取边缘区内免疫组化染色阳性反应区进行超微切片,电镜下观察边缘区MET-ENK阳性反应神经终末突触的超微结构. 结果免疫组织化学染色显示在壳核和苍白球之间有一条密集排列的MET-ENK免疫反应阳性细胞带,即纹状体边缘区;电镜下可见边缘区神经元为长梭形或纺锤形,细胞器较少.MET-ENK阳性反应神经终末突触主要有5个类型:轴-树突触、轴-棘突触、轴-体突触、轴-轴突触以及多种类型的复合型突触. 结论猴脑纹状体边缘区MET-ENK阳性免疫反应神经终末突触结构具有多样性和复杂性,明显不同于纹状体其他部分.
목적 관찰후뇌변연구갑류안산-뇌배태(MET-ENK)양성면역반응신경종말돌촉적초미결구,위진일보탐색기학습기억공능제공초미결구기출. 방법 선취건강성년웅성항하후6지,상규관주고정화취뇌,빙동절편후행면역조직화학염색검측문상체MET-ENK적표체,취변연구내면역조화염색양성반응구진행초미절편,전경하관찰변연구MET-ENK양성반응신경종말돌촉적초미결구. 결과 면역조직화학염색현시재각핵화창백구지간유일조밀집배렬적MET-ENK면역반응양성세포대,즉문상체변연구;전경하가견변연구신경원위장사형혹방추형,세포기교소.MET-ENK양성반응신경종말돌촉주요유5개류형:축-수돌촉、축-극돌촉、축-체돌촉、축-축돌촉이급다충류형적복합형돌촉. 결론 후뇌문상체변연구MET-ENK양성면역반응신경종말돌촉결구구유다양성화복잡성,명현불동우문상체기타부분.
Objective Marginal division (MrD) of striatum is a universal structure in the mammalian brain,and it plays an critical role in learning and memory.In the present study,we try to investigate the synaptic ultrastructure of Methionine enkephalin (MET-ENK) immunoreacted fibers connected with neurons in the marginal division of the striatum in the monkey brains to explore the ultrastructural basis of the mechanism of learning and memory function in MrD.Methods Six male monkeys (macaque) were perfused with paraformaldehyde through heart to fix the brain and the brains were sectioned by a cryostat.Sections of the brains were performed immunohistochemical staining to detect the MET-ENK expression in the stfiatum; the areas with positive immumohistochemical staining was performed ultrastructural observation for morphological characteristics of the MET-ENK synapses in the MrD.Results Immunohistochemistry staining showed a dense arrangement of MET-ENK immunoreactive cells between the putamen and globus pallidus.Five major types of MET-ENK synapses were identified in the MrD:the axo-dendritic synapses,the axo-spinous synapses,the axo-somatic synapses,the axo-axonic synapses and the compound synapses.Conclusion The MET-ENK synapses in the MrD are diverse and complex,and can be distinguished from the rest of the striatum.