CAJ | 학술논문

目的观察颈内动脉输注骨髓间充质干细胞(BMSCs)对血管性痴呆(VD)大鼠学习记忆能力以及对血清神经元特异性烯醇化酶(NSE)和S-100蛋白含量的影响. 方法取幼年雄性清洁Wistar大鼠股骨骨髓单个核细胞(BMNCs),贴壁培养后取3～4代BMSCs诱导分化.老龄(18～24月龄)Wistar大鼠90只按随机数字表法分为对照组、模型组和治疗组,每组又分为2、4、8周3个时相点,每时相点各10只.后两组采用4VO改良法制作成VD大鼠模型,治疗组于术后24 h颈内动脉移植0.5 mL 1.2×107/mL的BMSCs.术后2、4、8周采用穿梭箱测试对各组大鼠学习记忆能力进行分析,采用ELISA法对各组大鼠血清NSE和S-100蛋白含量进行检测. 结果 (1)术后2,4,8周模型组大鼠主动回避反应(AAR)比率低于对照组,而治疗组较模型组明显提高,差异均有统计学意义(P＜0.05).(2)术后2周治疗组大鼠血清NSE含量较模型组明显降低,术后4、8周明显升高,差异均有统计学意义(P＜0.05).(3)术后各时相点治疗组大鼠血清S-100蛋白含量均明显低于模型组,差异有统计学意义(P＜0.05),与对照组相比差异均无统计学意义(P＞0.05). 结论颈内动脉输注BMSCs可明显改善VD大鼠学习记忆能力,显著降低血清NSE、S-100含量,从而有效减轻神经元、胶质细胞的损伤程度,对神经细胞修复、增殖及突触联系起到良好的保护作用.
목적 관찰경내동맥수주골수간충질간세포(BMSCs)대혈관성치태(VD)대서학습기억능력이급대혈청신경원특이성희순화매(NSE)화S-100단백함량적영향. 방법 취유년웅성청길Wistar대서고골골수단개핵세포(BMNCs),첩벽배양후취3～4대BMSCs유도분화.노령(18～24월령)Wistar대서90지안수궤수자표법분위대조조、모형조화치료조,매조우분위2、4、8주3개시상점,매시상점각10지.후량조채용4VO개량법제작성VD대서모형,치료조우술후24 h경내동맥이식0.5 mL 1.2×107/mL적BMSCs.술후2、4、8주채용천사상측시대각조대서학습기억능력진행분석,채용ELISA법대각조대서혈청NSE화S-100단백함량진행검측. 결과 (1)술후2,4,8주모형조대서주동회피반응(AAR)비솔저우대조조,이치료조교모형조명현제고,차이균유통계학의의(P＜0.05).(2)술후2주치료조대서혈청NSE함량교모형조명현강저,술후4、8주명현승고,차이균유통계학의의(P＜0.05).(3)술후각시상점치료조대서혈청S-100단백함량균명현저우모형조,차이유통계학의의(P＜0.05),여대조조상비차이균무통계학의의(P＞0.05). 결론 경내동맥수주BMSCs가명현개선VD대서학습기억능력,현저강저혈청NSE、S-100함량,종이유효감경신경원、효질세포적손상정도,대신경세포수복、증식급돌촉련계기도량호적보호작용.
Objective To investigate the effect of intracarotid administration of bone marrow stem cells (BMSCs) on learning and memory abilities of vascular dementia (VD) rats and the variability of expressions of neuron-specific enolase (NSE) and S-100 protein in the serum.Methods BMNCs collected from clean juvenile male Wistar rat femur were isolated; after adherent culture,the 3-4th generation BMSCs were performed induced differentiation.Ninety Wistar rats (age:18 to 24 months old) were randomly divided into three groups:control group,model group and treatment group,and each group was further classified into three subgroups by means of survival times of 2,4 and 8 weeks,which means that every subgroup consisted often rats.All VD rats,which were modeled by modified Pulsinellis 4-vessel occlusion (4VO); and those in the treatment group were injected 0.5 mL 1.2×107/mL BMSCs into the internal carotid artery 24 after operation.The learning and memory abilities of each rat in all subgroups were analyzed by shuttle box testing and the NSE leves and S-100 protein in serum were detected by enzyme-linked immuno sorbent assay (ELISA).Results The mean active avoidance response (AAR) ratios of the rats in the three subgroups (survival time of 2,4,8 weeks) of the model group,were,respectively,lower than those of the control group (P＜0.05),and those of the treatment group were significantly higher than those of the model group (P＜0.05).The serum levels of NSE in the treatment group was significantly lower than that in the model group two weeks after operation (P＜0.05),but it reversed 4 and 8 weeks after operation.The S-100 protein expressions in the treatment group was statistically lower than that in the model group (P＜0.05),however,there is no significant difference between treatment group and control group (P＞0.05).Conclusion Intracarotid administration of BMSCs obviously improves the learning and memory abilities of the VD rats,remarkably reduces the serum levels of NSE and S-100 protein,and efficiently relieves the injury degree of neurons and glial cells,which indicates that BMSCs therapy has effective protection on neural regeneration,neuronal proliferation and synaptic connectivity.