中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2014年
2期
185-187
,共3页
杨辅直%李伟生%苏格炘%张倩%陈晓玲%刘红铃%闵宇懿
楊輔直%李偉生%囌格炘%張倩%陳曉玲%劉紅鈴%閔宇懿
양보직%리위생%소격흔%장천%진효령%류홍령%민우의
复杂型热性惊厥%单纯型热性惊厥%脑源性神经营养因子%热休克蛋白70%儿童
複雜型熱性驚厥%單純型熱性驚厥%腦源性神經營養因子%熱休剋蛋白70%兒童
복잡형열성량궐%단순형열성량궐%뇌원성신경영양인자%열휴극단백70%인동
Complicated febrile convulsion%Simple febrile convulsion%Brain derived neurotrophic factor%Heat shock protein 70%Children
目的 探讨复杂型热性惊厥患儿血清脑源性神经营养因子(BDNF)及热休克蛋白70(HSP70)水平在严重惊厥时脑缺氧损伤中的作用和意义. 方法 选择自2010年10月至2012年12月武警广东省总队医院儿科收治的43例复杂型热性惊厥患儿、41例单纯型热性惊厥患儿以及同期36例体检健康儿童(正常对照组)为研究对象,应用双抗体夹心酶联免疫吸附法(ELISA)测定血清BDNF、HSP70水平,并与复杂型热性惊厥组患儿抽搐持续时间、抽搐次数进行相关性分析. 结果 复杂型热性惊厥组患儿血清BDNF、HSP70水平明显升高,与单纯型热性惊厥组和正常对照组相比差异均有统计学意义(P＜0.05),而单纯型热性惊厥组和正常对照组之间差异无统计学意义(P＞0.05).复杂型热性惊厥组患儿血清BDNF、HSP70水平与其抽搐持续时间、抽搐次数之间均呈正相关关系(r=0.553,P=-0.015;r=0.587.P=0.012;r=0.526,P=0.024;r=0.562,P=0.018). 结论 复杂性热性惊厥存在一定程度的脑损伤,BDNF、HSP70可能参与其损伤修复机制.
目的 探討複雜型熱性驚厥患兒血清腦源性神經營養因子(BDNF)及熱休剋蛋白70(HSP70)水平在嚴重驚厥時腦缺氧損傷中的作用和意義. 方法 選擇自2010年10月至2012年12月武警廣東省總隊醫院兒科收治的43例複雜型熱性驚厥患兒、41例單純型熱性驚厥患兒以及同期36例體檢健康兒童(正常對照組)為研究對象,應用雙抗體夾心酶聯免疫吸附法(ELISA)測定血清BDNF、HSP70水平,併與複雜型熱性驚厥組患兒抽搐持續時間、抽搐次數進行相關性分析. 結果 複雜型熱性驚厥組患兒血清BDNF、HSP70水平明顯升高,與單純型熱性驚厥組和正常對照組相比差異均有統計學意義(P＜0.05),而單純型熱性驚厥組和正常對照組之間差異無統計學意義(P＞0.05).複雜型熱性驚厥組患兒血清BDNF、HSP70水平與其抽搐持續時間、抽搐次數之間均呈正相關關繫(r=0.553,P=-0.015;r=0.587.P=0.012;r=0.526,P=0.024;r=0.562,P=0.018). 結論 複雜性熱性驚厥存在一定程度的腦損傷,BDNF、HSP70可能參與其損傷脩複機製.
목적 탐토복잡형열성량궐환인혈청뇌원성신경영양인자(BDNF)급열휴극단백70(HSP70)수평재엄중량궐시뇌결양손상중적작용화의의. 방법 선택자2010년10월지2012년12월무경광동성총대의원인과수치적43례복잡형열성량궐환인、41례단순형열성량궐환인이급동기36례체검건강인동(정상대조조)위연구대상,응용쌍항체협심매련면역흡부법(ELISA)측정혈청BDNF、HSP70수평,병여복잡형열성량궐조환인추휵지속시간、추휵차수진행상관성분석. 결과 복잡형열성량궐조환인혈청BDNF、HSP70수평명현승고,여단순형열성량궐조화정상대조조상비차이균유통계학의의(P＜0.05),이단순형열성량궐조화정상대조조지간차이무통계학의의(P＞0.05).복잡형열성량궐조환인혈청BDNF、HSP70수평여기추휵지속시간、추휵차수지간균정정상관관계(r=0.553,P=-0.015;r=0.587.P=0.012;r=0.526,P=0.024;r=0.562,P=0.018). 결론 복잡성열성량궐존재일정정도적뇌손상,BDNF、HSP70가능삼여기손상수복궤제.
Objective To investigate the serum levels of brain derived neurotrophic factor (BDNF) and heat shock protein 70 (HSP70),and evaluate their clinical significance in brain injury of children with complicated febrile convulsions (CFC).Methods Forty-three patients with CFC,41 children with simple febrile convulsions and 36 healthy controls were recruited in this study; their serum levels of BDNF and HSP70 were detected by enzyme linked immunosorbent assay (ELISA); correlation between levels of BDNF and HSP70 and both time of twitch duration and twitch frequency were analyzed.Results The mean serum concentrations of BDNF (4.96±1.21 ng/mL) and HSP70 (2.86± 0.93 ng/mL) in patients of CFC group were significantly higher than those in patients of simple febrile convulsions and healthy controls (P＜0.05); while no significant difference was noted between patients of simple febrile convulsions and healthy controls (P＞0.05).These changes were closely related to the duration time and frequency of convulsions(r=0.553,P=0.015; r=0.587,P=0.012; r=0.526,P=0.024; r=0.562,P=0.018).Conclusions It is sure that some brain injury exists in children with CFC; BDNF and HSP-70 might be one of the molecular mechanisms for the brain injury and protective effect.
