CAJ | 학술논문

目的比较不同途径联合移植骨髓间充质干细胞-硫酸软骨素酶修复家兔脊髓损伤(SCI)的疗效差异. 方法 24只SCI家兔按照随机数字表法分为损伤对照(IC)组、椎管内移植(IS)组、静脉移植(IV)组和动脉移植(IA)组,每组6只.后3组分别于SCI后1周经相应途径联合移植骨髓间充质干细胞-硫酸软骨素酶,IC组仅制作损伤模型,不行其他处理.SCI前及SCI后1、2、3、4周通过动物行为学评估、神经电生理检测、荧光示踪技术及免疫组化等方法,比较4组家兔疗效差异. 结果 (1)改良Tarlov评分:SCI后3周,IA组Tarlov评分与IC组比较明显升高,差异有统计学意义(P＜0.05);术后4周,各移植组Tarlov评分明显优于IC组,差异均有统计学意义(p＜0.05).(2)HE染色:各组损伤处均有大量空泡形成,相对于IC组,各移植组损伤处空泡较小,并可见部分神经纤维束通过.(3)免疫组化:各移植组神经丝蛋白表达量均明显强于IC组;IS组及IA组表达量明显高于IV组,差异均有统计学意义(P＜0.05).胶质纤维酸性蛋白阳性表达面积方面,各移植组均低于IC组;而与IS和IA组相比IV组表达面积进一步减少,差异均有统计学意义(P＜0.05).(4)荧光双染比较:SCI后4周,各移植组镜下均可见较多的PKH-DAPI阳性细胞.其中IS和IA组定植率(28.50％、27.75％)相比IV组(21.75％)明显增高,差异有统计学意义(P＜0.05);(5)皮层感觉诱发电位潜伏期及波幅比较:SCI后第3、4周,各移植组潜伏期及波幅均优于IC组,差异有统计学意义(P＜0.05);其中SCI后4周,IS组及IA组潜伏期及波幅更优于IV组,差异有统计学意义(P＜0.05). 结论 3种途径移植治疗对于SCI均有明显疗效,其中肋间动脉移植途径及椎管内移植疗效更为明显.
목적 비교불동도경연합이식골수간충질간세포-류산연골소매수복가토척수손상(SCI)적료효차이. 방법 24지SCI가토안조수궤수자표법분위손상대조(IC)조、추관내이식(IS)조、정맥이식(IV)조화동맥이식(IA)조,매조6지.후3조분별우SCI후1주경상응도경연합이식골수간충질간세포-류산연골소매,IC조부제작손상모형,불행기타처리.SCI전급SCI후1、2、3、4주통과동물행위학평고、신경전생리검측、형광시종기술급면역조화등방법,비교4조가토료효차이. 결과 (1)개량Tarlov평분:SCI후3주,IA조Tarlov평분여IC조비교명현승고,차이유통계학의의(P＜0.05);술후4주,각이식조Tarlov평분명현우우IC조,차이균유통계학의의(p＜0.05).(2)HE염색:각조손상처균유대량공포형성,상대우IC조,각이식조손상처공포교소,병가견부분신경섬유속통과.(3)면역조화:각이식조신경사단백표체량균명현강우IC조;IS조급IA조표체량명현고우IV조,차이균유통계학의의(P＜0.05).효질섬유산성단백양성표체면적방면,각이식조균저우IC조;이여IS화IA조상비IV조표체면적진일보감소,차이균유통계학의의(P＜0.05).(4)형광쌍염비교:SCI후4주,각이식조경하균가견교다적PKH-DAPI양성세포.기중IS화IA조정식솔(28.50％、27.75％)상비IV조(21.75％)명현증고,차이유통계학의의(P＜0.05);(5)피층감각유발전위잠복기급파폭비교:SCI후제3、4주,각이식조잠복기급파폭균우우IC조,차이유통계학의의(P＜0.05);기중SCI후4주,IS조급IA조잠복기급파폭경우우IV조,차이유통계학의의(P＜0.05). 결론 3충도경이식치료대우SCI균유명현료효,기중륵간동맥이식도경급추관내이식료효경위명현.
Objective To compare the effects of different treatment routes on spinal cord injuries (SCI) in rabbits by co-transplantation of bone mesenchymal stem cells (BMSCs) with chondroitin sulfate enzymes (ChABC).Methods Twenty-four SCI rabbits were randomized into injury control group (IC),intraspinal treatment group (IS),intravenous treatment group (IV) and intraarterial treatment group (IA,n=6); BMSCs combined with ChABC were transplanted to the spinal cord tissue through the above three routes one week after SCI,respectively.Animal behavior evaluation,neural electrophysiology examination,and fluorescence tracer and immunohistochemical methods were used to compare with curative effects of co-transplantation via different routes in rabbit spinal cord injuries before SCI,and 1,2,3 and 4 weeks after SCI.Results Modified Tarlov scale scores:three weeks after SCI,Tarlov scale scores in IA group significantly increased as compared with that of IC group (P＜0.05); four weeks after SCI,three treatment groups recovered more significantly than IC group (P＜0.05).Hematoxylin-Eosin (HE) staining showed that large vacuoles were found at the spinal cord tissues of the IC group,while smaller vacuoles and visible part of the nerve fibers passing through were noted at the three treatment groups.Immunohistochemical method indicated that the expression of neurofilament 200 (NF200) was obviously more intense and that of glial fibrillary acidic portein (GFAP) was significantly weaker in the three treatment groups as compared with those in IC group (P＜0.05); IS group and IA group had more intense NF200 expression than that in IV group (P＜0.05); for GFAP expression,the highest expression was found in IC group,while that expression in IS and IA group was weaker and group IV was much obvious,with significant differences (P＜0.05).Double staining comparison:four weeks after SCI,many PKH-DAPI positive cells were seen in the three treatment groups under high magnification,and the migration ratios of the IS and IA group were higher than those of IV group,with statistically significant differences (P＜0.05).Latency period and wave amplitude in somatosensory evoked potential:third and fourth week after injury,the latency period and wave amplitude of the three treatment groups recovered better than those ofIC group with significant differences (P＜0.05); four weeks after SCI,IS group and IA group recovered better than IV group with statistically significant differences (P＜0.05).Conclusions The above three transplantation routes achieve good curative effect.Delivery though IS and IA routes are more effective than IV route.