CAJ | 학술논문

目的探讨大鼠创伤性颅脑损伤的程度与血清单核细胞趋化因子(MCP-1)、血管内皮生长因子(VEGF)及损伤区CD34+细胞数量的相关性. 方法 80只SD大鼠按随机数字表法分为对照组、假手术组、轻度损伤组和重度损伤组,每组20只.采用Feeney法制作大鼠创伤性颅脑损伤模型.伤后3、7d进行改良大鼠神经功能缺损评分(mNSS)评价神经功能;伤后6h、1d、3d、7d行多聚甲醛灌注取脑组织,行HE染色并采用免疫组织化学染色(SP法)检测各组大鼠脑损伤区CD34+细胞的表达情况;伤后1h、3h、6h、9h、1d、3d、5d、7d取外周血,采用酶联免疫吸附法(ELISA)测定外周血血清MCP-1、VEGF的水平. 结果与轻度损伤组比较,重度损伤组伤后3d、7 dmNSS评分较高,差异有统计学意义(P＜0.05);免疫组化染色显示对照组、假手术组大鼠脑损伤区CD34+细胞表达极少,轻、重度损伤组损伤区均有CD34+细胞出现,且轻度损伤组损伤区CD34+细胞数量较多;轻度损伤组、重度损伤组血清MCP-1、VEGF表达水平在各时间点均较对照组、假手术组高,且重度损伤组血清MCP-1表达水平高于轻度损伤组,VEGF表达水平明显低于轻度损伤组,差异均有统计学意义(P＜0.05). 结论大鼠血清MCP-1表达量与颅脑损伤程度呈正相关,血清VEGF表达量、脑损伤区CD34+细胞数量与颅脑损伤程度呈负相关,这些可作为评判大鼠创伤性颅脑损伤程度的观察指标.
목적 탐토대서창상성로뇌손상적정도여혈청단핵세포추화인자(MCP-1)、혈관내피생장인자(VEGF)급손상구CD34+세포수량적상관성. 방법 80지SD대서안수궤수자표법분위대조조、가수술조、경도손상조화중도손상조,매조20지.채용Feeney법제작대서창상성로뇌손상모형.상후3、7d진행개량대서신경공능결손평분(mNSS)평개신경공능;상후6h、1d、3d、7d행다취갑철관주취뇌조직,행HE염색병채용면역조직화학염색(SP법)검측각조대서뇌손상구CD34+세포적표체정황;상후1h、3h、6h、9h、1d、3d、5d、7d취외주혈,채용매련면역흡부법(ELISA)측정외주혈혈청MCP-1、VEGF적수평. 결과 여경도손상조비교,중도손상조상후3d、7 dmNSS평분교고,차이유통계학의의(P＜0.05);면역조화염색현시대조조、가수술조대서뇌손상구CD34+세포표체겁소,경、중도손상조손상구균유CD34+세포출현,차경도손상조손상구CD34+세포수량교다;경도손상조、중도손상조혈청MCP-1、VEGF표체수평재각시간점균교대조조、가수술조고,차중도손상조혈청MCP-1표체수평고우경도손상조,VEGF표체수평명현저우경도손상조,차이균유통계학의의(P＜0.05). 결론 대서혈청MCP-1표체량여로뇌손상정도정정상관,혈청VEGF표체량、뇌손상구CD34+세포수량여로뇌손상정도정부상관,저사가작위평판대서창상성로뇌손상정도적관찰지표.
Objective To explore the correlations between degrees of traumatic brain injury and expressions of serum monocyte chemoattractant protein-1 (MCP-1),and serum vascular endothelial growth factor (VEGF),and number of CD34+ cells of the damage zone in rats.Methods Eighty clean adult SD rats were randomly divided into control group,sham-operated group,slight injury group and severe injury group (n=20).Feeney protocol was employed to establish the rat brain traumatic models.Modified neurological severity scale (mNSS) was used to evaluate the neurological function 3 and 7 d after the injury; the peripheral blood was collected at 1,3,6 and 9 h,and 1,3,5 and 7 d after the injury,and the expressions of serum MCP-1 and VEGF were detected by enzyme-linked immuno sorbent assay (ELISA).After the rats being affused with paraformaldehyde,the brain tissues were collected at 6 h,1,3 and 7 d after the injury; the CD34+ cells in the injury areas were detected by immunohistochemistry (SP staining).Results As compared with the rats in the slight injury group,those in the severe injury group had significantly higher mNSS scores (P＜0.05).Immunohistochemistry showed that the number of CD34+ cells of the damage zone was small in control group and sham-operated group; the CD34+ cells appeared in the injury area of slight injury group and severe injury group,and the amount ofCD34+ cells in severe injury group was smaller than that in the slight injury group.ELISA showed the expressions of MCP-1 and VEGF in peripheral blood in the control group and sham-operated group were significantly lower than those in the slight injury group and severe injury group at all observation time points (P＜0.05);the expression of MCP-1 in the severe injury group was significantly higher than that in the slight injury group (P＜0.05); the expression of VEGF in the slight injury group was statistically higher than that in the severe injury group (P＜0.05).Conclusion The expression of serum MCP-1 is positively correlated to the severity of brain trauma injury; the expression of serum VEGF and the number ofCD34+ cells in the area of brain injury are negatively correlated to the severity of brain trauma injury; all of these indices could be used to evaluate the severity of traumatic brain injury in rats.