中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2014年
4期
336-339
,共4页
沈晓燕%房晓%蒋磊%盛平%丁茂华%仇冠中%吴小军%胡国汉
瀋曉燕%房曉%蔣磊%盛平%丁茂華%仇冠中%吳小軍%鬍國漢
침효연%방효%장뢰%성평%정무화%구관중%오소군%호국한
神经胶质瘤%周期素依赖性蛋白激酶亚单位1%S期激酶相关蛋白2%p27%免疫组织化学染色
神經膠質瘤%週期素依賴性蛋白激酶亞單位1%S期激酶相關蛋白2%p27%免疫組織化學染色
신경효질류%주기소의뢰성단백격매아단위1%S기격매상관단백2%p27%면역조직화학염색
Glioma%Cyclin kinase subunit 1%S-phase kinase associated protein 2%P27%Immunohistochemistry
目的 研究周期素依赖性蛋白激酶亚单位1(Cks1)、S期激酶相关蛋白2(Skp2)和周期素依赖性蛋白激酶抑制物p27在人脑胶质瘤中的表达及相互关系,同时探讨Cks1对胶质瘤恶性生物学进展的可能作用机制.方法 收集上海长征医院神经外科自2009年6月至2011年6月手术切除的胶质瘤组织标本34例及颅脑外伤内减压术中切除的非肿瘤脑组织10例,运用免疫组化EnVision法检测Cks1、Skp2和p27的蛋白表达水平并比较相互关系.结果 正常脑组织、低级别(WHO Ⅰ、Ⅱ级)和高级别(WHOⅢ、Ⅳ级)胶质瘤中Cks1蛋白表达的免疫反应评分(IRS)分别为4.90±2.38,3.20±2.98和2.16±2.52,随胶质瘤病理级别进展表达下降,三者差异有统计学意义(P<0.05).Skp2在正常脑组织中无表达,低级别和高级别胶质瘤中的IRS为1.00±1.13和2.26±2.23,随胶质瘤病理级别进展表达增加,差异有统计学意义(P<0.05).正常脑组织中p27表达IRS为7.80±0.63,低级别和高级别胶质瘤中IRS分别为5.00±2.10和2.21±1.47,差异有统计学意义(P<0.05).34例胶质瘤组织标本中,Cks1表达与p27表达呈正相关(r=0.380,P=0.027),Skp2表达水平与Cks1、p27均无相关性(r=0.080,P=0.662; r=-0.160,P=-0.376).结论 Cks1、Skp2和p27可作为评价胶质瘤良恶性程度的参考指标;Cks1可能通过促进p27蛋白聚集起到抑制脑胶质瘤恶性进展作用.
目的 研究週期素依賴性蛋白激酶亞單位1(Cks1)、S期激酶相關蛋白2(Skp2)和週期素依賴性蛋白激酶抑製物p27在人腦膠質瘤中的錶達及相互關繫,同時探討Cks1對膠質瘤噁性生物學進展的可能作用機製.方法 收集上海長徵醫院神經外科自2009年6月至2011年6月手術切除的膠質瘤組織標本34例及顱腦外傷內減壓術中切除的非腫瘤腦組織10例,運用免疫組化EnVision法檢測Cks1、Skp2和p27的蛋白錶達水平併比較相互關繫.結果 正常腦組織、低級彆(WHO Ⅰ、Ⅱ級)和高級彆(WHOⅢ、Ⅳ級)膠質瘤中Cks1蛋白錶達的免疫反應評分(IRS)分彆為4.90±2.38,3.20±2.98和2.16±2.52,隨膠質瘤病理級彆進展錶達下降,三者差異有統計學意義(P<0.05).Skp2在正常腦組織中無錶達,低級彆和高級彆膠質瘤中的IRS為1.00±1.13和2.26±2.23,隨膠質瘤病理級彆進展錶達增加,差異有統計學意義(P<0.05).正常腦組織中p27錶達IRS為7.80±0.63,低級彆和高級彆膠質瘤中IRS分彆為5.00±2.10和2.21±1.47,差異有統計學意義(P<0.05).34例膠質瘤組織標本中,Cks1錶達與p27錶達呈正相關(r=0.380,P=0.027),Skp2錶達水平與Cks1、p27均無相關性(r=0.080,P=0.662; r=-0.160,P=-0.376).結論 Cks1、Skp2和p27可作為評價膠質瘤良噁性程度的參攷指標;Cks1可能通過促進p27蛋白聚集起到抑製腦膠質瘤噁性進展作用.
목적 연구주기소의뢰성단백격매아단위1(Cks1)、S기격매상관단백2(Skp2)화주기소의뢰성단백격매억제물p27재인뇌효질류중적표체급상호관계,동시탐토Cks1대효질류악성생물학진전적가능작용궤제.방법 수집상해장정의원신경외과자2009년6월지2011년6월수술절제적효질류조직표본34례급로뇌외상내감압술중절제적비종류뇌조직10례,운용면역조화EnVision법검측Cks1、Skp2화p27적단백표체수평병비교상호관계.결과 정상뇌조직、저급별(WHO Ⅰ、Ⅱ급)화고급별(WHOⅢ、Ⅳ급)효질류중Cks1단백표체적면역반응평분(IRS)분별위4.90±2.38,3.20±2.98화2.16±2.52,수효질류병리급별진전표체하강,삼자차이유통계학의의(P<0.05).Skp2재정상뇌조직중무표체,저급별화고급별효질류중적IRS위1.00±1.13화2.26±2.23,수효질류병리급별진전표체증가,차이유통계학의의(P<0.05).정상뇌조직중p27표체IRS위7.80±0.63,저급별화고급별효질류중IRS분별위5.00±2.10화2.21±1.47,차이유통계학의의(P<0.05).34례효질류조직표본중,Cks1표체여p27표체정정상관(r=0.380,P=0.027),Skp2표체수평여Cks1、p27균무상관성(r=0.080,P=0.662; r=-0.160,P=-0.376).결론 Cks1、Skp2화p27가작위평개효질류량악성정도적삼고지표;Cks1가능통과촉진p27단백취집기도억제뇌효질류악성진전작용.
Objective To investigate the expressions ofcyclin kinase subunit 1 (Cks1),S-phase kinase associated protein 2 (Skp2) and Cyclin-dependent kinase inhibitor p27 in human gliomas and their correlations,and study the possible role of Cks1 to glioma malignant progression.Methods Immunohistochemical staining (EnVision method) was used to measure the protein expressions of Cks 1,Skp2 and p27 in 34 human glioma samples and 10 normal brain tissue slices,collected in our hospital from June 2009 to June 2011.Relationships between these levels of these proteins were analyzed.Results The immunoreactivity scale (IRS) scores of Cksl expression in normal brain tissue,low grade gliomas and high grade gliomas were 4.90±2.38,3.20±2.98 and 2.16±2.52; Cksl expression was decreased as the tumor grade developed.Skp2 protein was negative in all 10 cases of normal brain tissues and the IRS scores of Skp2 expression in low and high grade gliomas were 1.00±1.13 and 2.26±2.23; they increased in a malignancy-dependent manner.The IRS scores of p27 expression in normal brain tissue,low grade gliomas and high grade gliomas were 7.80±0.63,5.00±2.10 and 2.21±1.47 with significant differences (P＜0.05).Significantly positive correlation was found only between expressions of Cksl and p27 (r=0.38,P＜0.05),while no significant correlations between expression of Cksl and Skp2 and expression of p27 and Skp2 were noted (r=0.08 and-0.16,P＞0.05).Conclusion Cks1,Skp2 and p27 can be used as markers to evaluate glioma histological grade; Cks1 could inhibit gliomas malignant development by promoting p27 accumulation.
