CAJ | 학술논문

目的探讨抑制磷酸腺苷活化蛋白激酶(AMPK)活性对小鼠脑缺血再灌注损伤后神经元的保护作用机制.方法 54只雄性C57BL/6小鼠,按随机数字表法分为假手术组、缺血再灌注组、缺血再灌注治疗组,每组18只.后2组采用线栓法建立小鼠大脑中动脉闭塞(MCAO)模型,缺血再灌注治疗组在插入线栓时腹腔注射AMPK抑制剂Compound C,假手术组及缺血再灌注组相同时间予腹腔注射等量生理盐水.应用HE染色观察3组小鼠缺血再灌注后组织病理学变化;应用免疫组化染色法、TUNEL染色法观察脑缺血再灌注损伤24 h后细胞色素C(CytC)的表达及神经元凋亡情况.结果与缺血再灌注组比较,缺血再灌注治疗组小鼠皮质及海马CA1区组织病理学损伤减轻,CytC阳性细胞数分别为(28.86±9.65)个/视野、(13.33±2.75)个/视野,差异均有统计学意义(P＜0.05);TUNEL阳性细胞数亦显著减少.结论抑制AMPK活性能够减轻脑缺血再灌注损伤,推测机制为通过抑制Cyt C表达减少神经元凋亡.
목적 탐토억제린산선감활화단백격매(AMPK)활성대소서뇌결혈재관주손상후신경원적보호작용궤제.방법 54지웅성C57BL/6소서,안수궤수자표법분위가수술조、결혈재관주조、결혈재관주치료조,매조18지.후2조채용선전법건립소서대뇌중동맥폐새(MCAO)모형,결혈재관주치료조재삽입선전시복강주사AMPK억제제Compound C,가수술조급결혈재관주조상동시간여복강주사등량생리염수.응용HE염색관찰3조소서결혈재관주후조직병이학변화;응용면역조화염색법、TUNEL염색법관찰뇌결혈재관주손상24 h후세포색소C(CytC)적표체급신경원조망정황.결과 여결혈재관주조비교,결혈재관주치료조소서피질급해마CA1구조직병이학손상감경,CytC양성세포수분별위(28.86±9.65)개/시야、(13.33±2.75)개/시야,차이균유통계학의의(P＜0.05);TUNEL양성세포수역현저감소.결론 억제AMPK활성능구감경뇌결혈재관주손상,추측궤제위통과억제Cyt C표체감소신경원조망.
Objective To investigate the effects of inhibition of adenosine monophosphate-activated protein kinase (AMPK) activity on neuronal apoptosis in mice after cerebral ischemia reperfusion injury.Methods Fifty-four male C57BL/6 mice were randomly divided into three groups (n=18):sham-operated group,ischemia reperfusion group and ischemia reperfusion therapy group.Mice models of middle cerebral artery occlusion (MCAO) in the later two groups were made by insertion of a thread through intemal carotid artery.Compound C was injected intraperitoneally in ischemia reperfusion therapy group when the thread was inserted.The same volume of saline was given to the sham-operated group and ischemia reperfusion group at the same time to intraperitonel injection.The Cytochrome C (Cy C) expression and neuronal apoptosis were observed by immunohistochemical staining and TUNEL 24 h after cerebral ischemia reperfusion injury.Results As compared with that in the ischemia reperfusion group,the histopathological damage was reduced in the hippocampal CA1 region of mice in the ischemia reperfusion therapy group; Cyt C positive cells in the two groups were (28.86±9.65)/field and (13.33±2.75)/field,respectively.TUNEL positive cells in the two groups were (67.14±8.55)/HP and (74.57±6.77)/HP,with significant difference (P＜0.05).Conclusion Inhibition of AMPK activity can decrease cerebral ischemia reperfusion injury,which is problely by lowering the Cyt C expression to decrease the neuronal apoptosis in mice.