CAJ | 학술논문

目的观察雪莲注射液对帕金森病(PD)模型小鼠炎症损伤的保护作用. 方法 C57BL/6小鼠108只按随机数字表法分为阴性对照组、PD模型组、阳性对照组(丹参注射液组)、雪莲注射液高剂量组[1.04 mL/(kg·d)]、中剂量组[0.52 mL/(kg·d)]、低剂量组[0.26 mL/(kg· d)],每组18只.PD小鼠模型通过腹腔注射百草枯(10 mg/kg)建立.各组小鼠在实验前及实验后7周行神经功能行为评估;采用高效液相法检测小鼠黑质-纹状体部位多巴胺(DA)含量;采用免疫组化染色方法检测小鼠黑质部位酪氨酸羟化酶(TH)、整合素α(mac-1)及肿瘤坏死因子-α(TNF-α)阳性细胞数. 结果实验7周后,PD模型组小鼠运动协调障碍明显,雪莲注射液高剂量组能够明显缓解.高效液相法检测结果显示,PD模型组DA含量较阴性对照组明显降低,差异有统计学意义(P＜0.05);雪莲注射液高剂量组DA的含量明显增加,达到(10.901.04) μg/g,与PD模型组相比差异有统计学意义(P＜0.05).免疫组化染色结果显示:PD模型组TH阳性细胞数较阴性对照组明显降低,mac-1阳性细胞数、TNF-α阳性细胞数较阴性对照组明显增加,差异均有统计学意义(P＜0.05).雪莲注射液高剂量组TH阳性细胞数较PD模型组明显增加,达到(65.18±6.00)个/视野;mac-1阳性细胞数及TNF-α阳性细胞数较PD模型组明显降低,分别为(93.18±5.33)个/视野、(92.73±23.21)个/视野,差异均有统计学意义(P＜0.05). 结论高剂量雪莲注射液能够明显改善百草枯所致PD小鼠的运动协调障碍,促进黑质部位DA能神经元的存活,其机制为抑制小鼠黑质部位小胶质细胞的活化及其介导的炎症反应.
목적 관찰설련주사액대파금삼병(PD)모형소서염증손상적보호작용. 방법 C57BL/6소서108지안수궤수자표법분위음성대조조、PD모형조、양성대조조(단삼주사액조)、설련주사액고제량조[1.04 mL/(kg·d)]、중제량조[0.52 mL/(kg·d)]、저제량조[0.26 mL/(kg· d)],매조18지.PD소서모형통과복강주사백초고(10 mg/kg)건립.각조소서재실험전급실험후7주행신경공능행위평고;채용고효액상법검측소서흑질-문상체부위다파알(DA)함량;채용면역조화염색방법검측소서흑질부위락안산간화매(TH)、정합소α(mac-1)급종류배사인자-α(TNF-α)양성세포수. 결과 실험7주후,PD모형조소서운동협조장애명현,설련주사액고제량조능구명현완해.고효액상법검측결과현시,PD모형조DA함량교음성대조조명현강저,차이유통계학의의(P＜0.05);설련주사액고제량조DA적함량명현증가,체도(10.901.04) μg/g,여PD모형조상비차이유통계학의의(P＜0.05).면역조화염색결과현시:PD모형조TH양성세포수교음성대조조명현강저,mac-1양성세포수、TNF-α양성세포수교음성대조조명현증가,차이균유통계학의의(P＜0.05).설련주사액고제량조TH양성세포수교PD모형조명현증가,체도(65.18±6.00)개/시야;mac-1양성세포수급TNF-α양성세포수교PD모형조명현강저,분별위(93.18±5.33)개/시야、(92.73±23.21)개/시야,차이균유통계학의의(P＜0.05). 결론 고제량설련주사액능구명현개선백초고소치PD소서적운동협조장애,촉진흑질부위DA능신경원적존활,기궤제위억제소서흑질부위소효질세포적활화급기개도적염증반응.
Objective To investigate the effect of Xuelian injection on mice models with Parkinson' s disease (PD).Methods C57BL/6 mice were given paraquat (PQ) by peritoneal injection (10 mg/kg) to induce PD models.Then,they were randomly divided into six groups:PD group,negative control group,positive controlgroup,low-dose (0.26mL/kg·d),middle-dose (0.52 mL/kg· d) and high-dose (1.04 mL/kg· d) Xuelian injection groups.Pole test and open field activity test were used for assessment of behavior 7 weeks after the injection; the dopamine (DA) content in the substantia nigra pars compacta (SNc) was observed by high-performance liquid chromatography (HPLC).The expressions of tyrosine hydroxylase (TH),integrin o (mac-l) and tumor necrosis factor α (TNF-α) in the SNc were measured by immunohistochemical staining under optical microscope.Results Seven weeks after the injection,the movement coordination disorder was significant in the PD group,and that had partial remission in the high-dose Xuelian injection group.HPLC showed that the DA content in the PD group was significantly lower than that in the negative control group (P＜0.05); that in the high-dose Xuelian injection group ([10.90±1.04] μg/g) was significantly higher than that in the PD group (P＜0.05).Immunohistochemical staining indicated that PD group had significantly decreased number of TH positive cells and statistically increased numbers of mac-1 and TNF-α positive cells as compared with the negative control group (P＜0.05); high-dose Xuelian injection group had significantly increased number of TH positive cells (65.18±6.00) and statistically decreased numbers of mac-1 and TNF-α positive cells (93.18±5.33 and 92.73±23.21) as compared with the PD group (P＜0.05).Conclusions High-dose Xuelian injection can obviously improve the movement coordination disorder and survival of DA neurons in the SNc,whose protective effect might be related to the inhabitation of microglia mediated inflammation injury and oxidative stress.