CAJ | 학술논문

目的研究低频电刺激脚桥被盖核(PPTg)对偏侧帕金森病(PD)大鼠步态及运动能力的影响. 方法 32只雄性SD大鼠按随机数字表法分为对照组(n=8)和实验组(n=24);2组大鼠采用立体定向手术将6-羟基多巴胺注入大鼠右侧前脑内侧束(MFB),实验组大鼠在此基础上将电极植入右侧PPTg.分别于立体定向术前1周、术后3周对2组大鼠行Catwalk步态及旷场实验并采集数据;术后4周,实验组行低频电刺激PPTg后再次采集行为学数据. 结果 Catwalk步态实验结果显示,与术前比较,2组大鼠术后的双爪耦合值(右前→左前、右前→左后、右后→左前、右后→左后)均增加,术后速度变化百分比均增加,每秒步数均减少,差异有统计学意义(P＜0.05).与刺激前比较,实验组大鼠低频电刺激PPTg后双爪耦合值均减少,速度变化百分比减少,每秒步数增加,差异有统计学意义(P＜0.05).旷场实验中:实验组和对照组大鼠术后运动距离明显减少,实验组大鼠行低频电刺激PPTg后运动距离较刺激前明显增加,差异均有统计学意义(P＜0.05). 结论低频电刺激PPTg可改善偏侧PD大鼠步态和运动能力,Catwalk步态实验及旷场实验可有效检测该刺激作用的效果.
목적 연구저빈전자격각교피개핵(PPTg)대편측파금삼병(PD)대서보태급운동능력적영향. 방법 32지웅성SD대서안수궤수자표법분위대조조(n=8)화실험조(n=24);2조대서채용입체정향수술장6-간기다파알주입대서우측전뇌내측속(MFB),실험조대서재차기출상장전겁식입우측PPTg.분별우입체정향술전1주、술후3주대2조대서행Catwalk보태급광장실험병채집수거;술후4주,실험조행저빈전자격PPTg후재차채집행위학수거. 결과 Catwalk보태실험결과현시,여술전비교,2조대서술후적쌍조우합치(우전→좌전、우전→좌후、우후→좌전、우후→좌후)균증가,술후속도변화백분비균증가,매초보수균감소,차이유통계학의의(P＜0.05).여자격전비교,실험조대서저빈전자격PPTg후쌍조우합치균감소,속도변화백분비감소,매초보수증가,차이유통계학의의(P＜0.05).광장실험중:실험조화대조조대서술후운동거리명현감소,실험조대서행저빈전자격PPTg후운동거리교자격전명현증가,차이균유통계학의의(P＜0.05). 결론 저빈전자격PPTg가개선편측PD대서보태화운동능력,Catwalk보태실험급광장실험가유효검측해자격작용적효과.
Objective To study the effect of low frequency stimulation (LFS) in the pedunculopontine tegmental nucleus (PPTg) on gait and movement ability in unilateral lesioned Parkinson's disease(PD) rats.Methods Thirty-two male SD rats were randomly assigned to control group (6-hydroxydopamine treatment,n=8) and experimental group (6-hydroxydopamine treatment combined with electrode implantation,n=24).All subjects were processed with injection of 6-hydroxydopamine in the fight medial forebrain bundle (MFB) and simultaneously the rats in the experimental group were received an electrode implantation into the ipsilateral PPTg.Catwalk gait and open field test data were acquired at 1 week before stereotactic surgery and 3 weeks after operation in the rats of control and experimental groups; the behavioral data of rats in the experimental group were recorded again 4 weeks after operation when they were under ongoing LFS-PPTg.Results Catwalk results showed that the postoperative couplings values (right front→left front,right front→left hind,right hind →left front,right hind →left hind) were significantly increased in all subjects as compared with the preoperative ones (P＜0.05); while couplings value in rats of the experimental group after LFS-PPTg significantly reduced as compared with that before LFS-PPTg (P＜0.05).The postoperative run speed variation statistically increased and postoperative cadence significantly decreased in all rats as compared with the preoperative ones (P＜0.05); the run speed variation significantly decreased and cadence obviously increased in experimental group after LFS-PPTg as compared with those before LFS-PPTg (P＜0.05).Open field test:the moving distance of all subjects decreased significantly after operation; the distance in rats of the experimental group after LFS-PPTg increased significantly as compared with that before LFS-PPTg.Conclusion LFS-PPTg can effectively improve gait function and motor activity of unilateral PD rat models.