中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2014年
6期
586-589
,共4页
史进%齐平建%刘建生%于东%王华民%沈凤彪%王昆鹏
史進%齊平建%劉建生%于東%王華民%瀋鳳彪%王昆鵬
사진%제평건%류건생%우동%왕화민%침봉표%왕곤붕
神经胶质瘤%表皮生长因子受体2%基质金属蛋白酶-2%基质金属蛋白酶-9
神經膠質瘤%錶皮生長因子受體2%基質金屬蛋白酶-2%基質金屬蛋白酶-9
신경효질류%표피생장인자수체2%기질금속단백매-2%기질금속단백매-9
Glioma%Epidermal growth factor receptor 2%Matrix metalloprotease-2%Matrix metalloprotease-9
目的 探讨人类表皮生长因子受体2(ErbB2)表达对人脑胶质瘤细胞侵袭能力的影响及其可能机制. 方法 在体外培养的胶质瘤TJ905细胞中分别转染ErbB2 shRNA(ErbB2 shRNA 载体组)和ErbB2过表达载体(ErbB2过表达载体组),以下调或上调细胞中ErbB2表达水平,并以转染空载体质粒(ErbB2空载对照组)为对照.应用Western blotting检测ErbB2及基质金属蛋白酶-2(MMP-2)、MMP-9蛋白的表达水平,应用Transwell小室侵袭实验评价各组细胞侵袭能力的变化.结果 与ErbB2空载对照组ErbB2及MMP-2、MMP-9蛋白表达水平(62.34 ±5.72,62.34±5.72,69.76±6.25)相比,转染ErbB2 shRNA载体后有效抑制了细胞中ErbB2及MMP-2、MMP-9蛋白表达(34.82±4.91,58.73±4.48,52.32±5.23),而转染ErbB2过表达载体后细胞中ErbB2及MMP-2、MMP-9蛋白表达水平明显升高(69.76±6.25,87.34±7.96,94.39±6.12),差异均有统计学意义(P＜0.05).ERRB2 shRNA载体组穿过上室Matrigel胶的平均细胞数(28.5个/视野)较ErbB2空载对照组(70个/视野)明显减少,而ERRB2过表达载体组(82个/视野)较ErbB2空载对照组明显增多. 结论 ErbB2表达水平变化可影响胶质瘤细胞的侵袭能力,其机制可能与MMP-2、MMP-9表达水平发生改变相关.
目的 探討人類錶皮生長因子受體2(ErbB2)錶達對人腦膠質瘤細胞侵襲能力的影響及其可能機製. 方法 在體外培養的膠質瘤TJ905細胞中分彆轉染ErbB2 shRNA(ErbB2 shRNA 載體組)和ErbB2過錶達載體(ErbB2過錶達載體組),以下調或上調細胞中ErbB2錶達水平,併以轉染空載體質粒(ErbB2空載對照組)為對照.應用Western blotting檢測ErbB2及基質金屬蛋白酶-2(MMP-2)、MMP-9蛋白的錶達水平,應用Transwell小室侵襲實驗評價各組細胞侵襲能力的變化.結果 與ErbB2空載對照組ErbB2及MMP-2、MMP-9蛋白錶達水平(62.34 ±5.72,62.34±5.72,69.76±6.25)相比,轉染ErbB2 shRNA載體後有效抑製瞭細胞中ErbB2及MMP-2、MMP-9蛋白錶達(34.82±4.91,58.73±4.48,52.32±5.23),而轉染ErbB2過錶達載體後細胞中ErbB2及MMP-2、MMP-9蛋白錶達水平明顯升高(69.76±6.25,87.34±7.96,94.39±6.12),差異均有統計學意義(P＜0.05).ERRB2 shRNA載體組穿過上室Matrigel膠的平均細胞數(28.5箇/視野)較ErbB2空載對照組(70箇/視野)明顯減少,而ERRB2過錶達載體組(82箇/視野)較ErbB2空載對照組明顯增多. 結論 ErbB2錶達水平變化可影響膠質瘤細胞的侵襲能力,其機製可能與MMP-2、MMP-9錶達水平髮生改變相關.
목적 탐토인류표피생장인자수체2(ErbB2)표체대인뇌효질류세포침습능력적영향급기가능궤제. 방법 재체외배양적효질류TJ905세포중분별전염ErbB2 shRNA(ErbB2 shRNA 재체조)화ErbB2과표체재체(ErbB2과표체재체조),이하조혹상조세포중ErbB2표체수평,병이전염공재체질립(ErbB2공재대조조)위대조.응용Western blotting검측ErbB2급기질금속단백매-2(MMP-2)、MMP-9단백적표체수평,응용Transwell소실침습실험평개각조세포침습능력적변화.결과 여ErbB2공재대조조ErbB2급MMP-2、MMP-9단백표체수평(62.34 ±5.72,62.34±5.72,69.76±6.25)상비,전염ErbB2 shRNA재체후유효억제료세포중ErbB2급MMP-2、MMP-9단백표체(34.82±4.91,58.73±4.48,52.32±5.23),이전염ErbB2과표체재체후세포중ErbB2급MMP-2、MMP-9단백표체수평명현승고(69.76±6.25,87.34±7.96,94.39±6.12),차이균유통계학의의(P＜0.05).ERRB2 shRNA재체조천과상실Matrigel효적평균세포수(28.5개/시야)교ErbB2공재대조조(70개/시야)명현감소,이ERRB2과표체재체조(82개/시야)교ErbB2공재대조조명현증다. 결론 ErbB2표체수평변화가영향효질류세포적침습능력,기궤제가능여MMP-2、MMP-9표체수평발생개변상관.
Objective To investigate the effect of epidermal growth factor receptor 2 (ErbB2) expression on invasion ofglioma cells and its possible mechanism.Methods Glioma TJ905 cells were cultured in vitro; and ErbB2 shRNA and overexpression vectors were constructed and transfected into Glioma T J905 cells to down-regulate and up-regulate the ErbB2 expression levels; empty vector plasmid was also transfected into the TJ905 cells as control group.Invasive ability changes of T J905 cells were measured by Transwell assay,and the expression levels of matrix metalloprotease (MMP)-2 and MMP-9 were identified by Western blotting.Results As compared with the ErbB2,MMP-2 and MMP-9 protein expression levels in the control group (62.34±5.72,62.34±5.72 and 69.76±6.25),those in the ErbB2 shRNA group were significantly decreased (34.82±4.91,58.73±4.48 and 52.32±5.23),while those in the ErbB2 overexpression group were significantly increased (69.76±6.25,87.34±7.96 and 94.39±6.12),with significant differences (P＜0.05).The mean cells crossing Matrigel in the ErbB2 shRNA group (28.5 cells/field) were obviously decreased,and those in the ErbB2 overexpression group were increased (82 cells/field) as compared with those in the control group (70 cells/field),with significant differences (P＜0.05).Conclusion ErbB2 expression can affect the invasiveness ofglioma cells,which might be related to the expression changes of MMP-2 and MMP-9.
