CAJ | 학술논문

目的评估脐血源神经干细胞(UCBNSCs)移植治疗脊髓损伤(SCI)后大鼠后肢功能恢复情况,并通过基因芯片筛选出移植治疗后对照组和实验组差异表达基因,分析相关基因与损伤脊髓功能恢复的关系,以期为SCI的基因治疗打下基础. 方法 50只健康雌性SD大鼠按随机数字表法分为假手术组(n=10)、磷酸盐缓冲液(PBS)注射组(n=20)、UCBNSCs移植组(n=20).假手术组仅在显微镜下行T9-11椎板打开,不做其他处理.后2组打开T9-11椎板后横断脊髓,制作大鼠脊髓全断模型.1周后UCBNSCs移植组行UCBNSCs(1×109/L细胞悬浮液10μL)脑室穿刺移植,PBS注射组用PBS代替.移植术后1d、1周、2周、4周、6周、8周分别对3组大鼠行血脑屏障(BBB)行为评分.第8周分别取PBS注射组和UCBNSCs移植组各7只SD大鼠,取得SCI处组织样本,行基因芯片分析,筛选出差异表达基因. 结果 PBS注射组和UCBNSCs移植组造模后均出现了瘫痪、尾部活动障碍及排尿功能障碍等.随着时间的延长,2组后肢功能均有所恢复,在术后2周、4周、6周和8周2组间差异具有统计学意义(P＜0.05).芯片分析筛选出24个差异表达基因,其中表达上调有20个,表达下调有4个. 结论 UCBNSCs移植治疗大鼠SCI有确切疗效,通过基因芯片分析推测其可能通过分化为神经元、增加损伤微环境中神经营养因子表达、抑制炎症反应和促进损伤局部的氧供等方式发挥作用.
목적 평고제혈원신경간세포(UCBNSCs)이식치료척수손상(SCI)후대서후지공능회복정황,병통과기인심편사선출이식치료후대조조화실험조차이표체기인,분석상관기인여손상척수공능회복적관계,이기위SCI적기인치료타하기출. 방법 50지건강자성SD대서안수궤수자표법분위가수술조(n=10)、린산염완충액(PBS)주사조(n=20)、UCBNSCs이식조(n=20).가수술조부재현미경하행T9-11추판타개,불주기타처리.후2조타개T9-11추판후횡단척수,제작대서척수전단모형.1주후UCBNSCs이식조행UCBNSCs(1×109/L세포현부액10μL)뇌실천자이식,PBS주사조용PBS대체.이식술후1d、1주、2주、4주、6주、8주분별대3조대서행혈뇌병장(BBB)행위평분.제8주분별취PBS주사조화UCBNSCs이식조각7지SD대서,취득SCI처조직양본,행기인심편분석,사선출차이표체기인. 결과 PBS주사조화UCBNSCs이식조조모후균출현료탄탄、미부활동장애급배뇨공능장애등.수착시간적연장,2조후지공능균유소회복,재술후2주、4주、6주화8주2조간차이구유통계학의의(P＜0.05).심편분석사선출24개차이표체기인,기중표체상조유20개,표체하조유4개. 결론 UCBNSCs이식치료대서SCI유학절료효,통과기인심편분석추측기가능통과분화위신경원、증가손상미배경중신경영양인자표체、억제염증반응화촉진손상국부적양공등방식발휘작용.
Objective To assess the recovery of hind limbs function in rats with spinal cord injury (SCI) after being treated with umbilical cord blood neural stem cells (UCBNSCs) transplantation,and analyze the relationship between the differentially expressed genes identified by gene chips and functional recovery of the spinal cord to lay foundation for gene therapy of spinal cord injury.Methods Fifty healthy female SD rats were randomly divided into sham-operated group (n=10),PBS injection control group (n=20) and UCBNSCs transplantation group (n=20).Only opening the T9-11 vertebral plate was performed in sham-operated group under microscope,while the spinal cords of PBS injection control group and UCBNSCs transplantation group were cut offafter opening the T9-11 vertebral plate to induce rat models of spinal cord transection,UCBNSCs transplantation was performed through ventricular puncture (1 × 109/L cell suspension 10 μL) one week after the model making.Blood brain barrier (BBB) behavior scale of three groups was carried out 1 d and 1,2,4,6 and 8 w after transplantation.Seven SD rats from PBS control group and UCBNSCs transplantation group were sacrificed to get spinal cord injury tissue samples at 8 w after transplantation; gene chip analysis was used to identify the differentially expressed genes.Results The hind limbs function of SD rats did not appear obvious damage in sham-operated group.Symptoms of paralysis,tail activity obstacle and urination dysfunction appeared in PBS injection control group and UCBNSCs transplantation group.As time went on,the function of hind limbs slowly recovered:1 d and 1 w after transplantation,BBB scale scores showed no statistical difference between PBS injection control group and UCBNSCs transplantation group (P＞0.05); statistical significance appeared at 2,4,6 and 8 w after transplantation between the two groups (P＜0.05).Twenty-four differentially expressed genes were identified,including 20 being up-regulated and 4 being down-regulated.Conclusions UCBNSCs transplantations in the treatment of SCI have exact effect.With gene chip analysis,it is supposed that UCBNSCs may differentiate into neurons,increase the output of neurotrophic factor expression in the microenvironment,inhibit the local inflammation reaction and promote the oxygen supply,so as to boost the recovery of neurological function.