CAJ | 학술논문

目的探讨大鼠脑出血后血肿周围脑组织c-Jun氨基末端激酶(JNK)表达的变化,以及凝血酶抑制剂阿加曲班对JNK活化的影响. 方法第一部分:30只雄性SD大鼠按随机数字表法分为假手术组(6只,纹状体注射生理盐水50μL)和脑出血组(24只,纹状体注射自体血50 μL),脑出血组再按照不同取材时间点分为12h、1d、3d、7d共4个亚组(每亚组6只);应用Westernblotting检测各组各时间点磷酸化JNK(p-JNK)和JNK的表达变化.第二部分:36只雄性SD大鼠按随机数字表法分为假手术组(12只)、脑出血+生理盐水组(12只,造模后3h原位注射生理盐水20μL)和脑出血+阿加曲班组(12只,造模后3h原位注射阿加曲班20 μL);应用Western blotting和免疫组化染色检测各组造模后1d血肿周围脑组织p-JNK和JNK的表达. 结果 (1)与假手术组比较,脑出血组各亚组血肿周围脑组织p-JNK蛋白表达均明显增高,并在脑出血后1d达到高峰,差异有统计学意义(P＜0.05),而JNK蛋白表达无明显变化.(2)造模后1d,脑出血+阿加曲班组血肿周围脑组织p-JNK蛋白表达较脑出血+生理盐水组明显降低,差异有统计学意义(P＜0.05),而JNK蛋白表达无明显变化;免疫组化染色检测到的p-JNK蛋白表达与Western blotting结果相同. 结论脑出血可诱导血肿周围脑组织JNK活化,而阿加曲班可抑制这一过程.
목적 탐토대서뇌출혈후혈종주위뇌조직c-Jun안기말단격매(JNK)표체적변화,이급응혈매억제제아가곡반대JNK활화적영향. 방법 제일부분:30지웅성SD대서안수궤수자표법분위가수술조(6지,문상체주사생리염수50μL)화뇌출혈조(24지,문상체주사자체혈50 μL),뇌출혈조재안조불동취재시간점분위12h、1d、3d、7d공4개아조(매아조6지);응용Westernblotting검측각조각시간점린산화JNK(p-JNK)화JNK적표체변화.제이부분:36지웅성SD대서안수궤수자표법분위가수술조(12지)、뇌출혈+생리염수조(12지,조모후3h원위주사생리염수20μL)화뇌출혈+아가곡반조(12지,조모후3h원위주사아가곡반20 μL);응용Western blotting화면역조화염색검측각조조모후1d혈종주위뇌조직p-JNK화JNK적표체. 결과 (1)여가수술조비교,뇌출혈조각아조혈종주위뇌조직p-JNK단백표체균명현증고,병재뇌출혈후1d체도고봉,차이유통계학의의(P＜0.05),이JNK단백표체무명현변화.(2)조모후1d,뇌출혈+아가곡반조혈종주위뇌조직p-JNK단백표체교뇌출혈+생리염수조명현강저,차이유통계학의의(P＜0.05),이JNK단백표체무명현변화;면역조화염색검측도적p-JNK단백표체여Western blotting결과상동. 결론 뇌출혈가유도혈종주위뇌조직JNK활화,이아가곡반가억제저일과정.
Objective To study the expression of c-Jun N-terminal kinase (JNK) in the hematoma peripheral region of rat models after intracerebral hemorrhage (ICH) and explore the effect of thrombin inhibitor argathoban on JNK expression.Methods In first set,30 male SD rats weighed 250-300 g were randomized into sham-operated group (striatal injection of saline 50 μL,n=6) and ICH group (intracerebral infusion of autologous blood 50 μL at corpus striatum,n=24); rats in ICH group were divided into four subgroups (12 h,and 1,3 and 7 d after intracerebral infusion ofautologous blood,n=6);Western blotting was utilized to observe the changes of phosphorylated-JNK (p-JNK) and JNK at the variable groups and time points.In second set,36 male SD rats were randomized into sham-operated group (n=12),ICH+saline group (n=12) and ICH+argathoban group (giving 20 μL argathoban 3 h after intracerebral infusion ofautologous blood,n=12); Western blotting and immunohistostaining were used to observe the expressions of p-JNK and JNK in sham-operated group,ICH+saline group and ICH+argathoban group one day after intracerebral infusion ofautologous blood.Results (1) Western blotting indicated that the expression of p-JNK in the peripheral region of hematoma in ICH group was increased and reached its peak level one day after infusion,which was significantly higher than that in the sham-operated group (P＜0.05); while the expression level of JNK was not altered at different groups.(2)Western blotting indicated that the expression of p-JNK in the peripheral region of hematoma in ICH+argathoban group was statistically decreased as compared with that in the ICH+saline group one day after infusion (P＜0.05),while the expression level of JNK was not altered at different groups.These findings were consistent with the results of immunohistostaining.Conclusion ICH induces JNK activation in the peripheral region of hematoma and thrombin inhibitor argathoban can inhibit this process.