中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2014年
8期
817-819
,共3页
黄金山%吴原%秦超%李偲俊%刘夕霞%谭明会%覃维含%陈镇%何寿春
黃金山%吳原%秦超%李偲俊%劉夕霞%譚明會%覃維含%陳鎮%何壽春
황금산%오원%진초%리시준%류석하%담명회%담유함%진진%하수춘
高迁移率族蛋白1%Sombati癫痫细胞模型%蛋白免疫印迹
高遷移率族蛋白1%Sombati癲癇細胞模型%蛋白免疫印跡
고천이솔족단백1%Sombati전간세포모형%단백면역인적
High-mobility group box-1%Sombati's cell model%Western blotting
目的 研究Sombati癫痫细胞模型中高迁移率族蛋白1(HMGB1)的变化规律. 方法 新生SD乳鼠断头后迅速钝性分离出双侧海马,取海马神经元体外培养至第9天,将神经元分为模型组(无镁细胞外液处理)和对照组(正常细胞外液处理),应用Wester blotting技术检测海马神经元在接受相应处理12、24、72 h后HMGB1蛋白的表达变化情况. 结果 Wester blotting显示,在神经元接受相应处理后12、24 h,模型组细胞所含HMGB1量均明显低于对照组,差异有统计学意义(t=2.783,P=0.012;t=2.509,P=0.021); 72 h时模型组HMGB1表达量却明显高于对照组,差异具有统计学意义(t=4.112,P=0.006).模型组HMGB1蛋白表达量于造模后12h、24 h、72 h呈现逐渐增高趋势. 结论 Sombati癫痫细胞模型中HMGB1的表达呈时间依赖性,随时间延长表达增加,可能在癫痫的病理生理中起重要作用.
目的 研究Sombati癲癇細胞模型中高遷移率族蛋白1(HMGB1)的變化規律. 方法 新生SD乳鼠斷頭後迅速鈍性分離齣雙側海馬,取海馬神經元體外培養至第9天,將神經元分為模型組(無鎂細胞外液處理)和對照組(正常細胞外液處理),應用Wester blotting技術檢測海馬神經元在接受相應處理12、24、72 h後HMGB1蛋白的錶達變化情況. 結果 Wester blotting顯示,在神經元接受相應處理後12、24 h,模型組細胞所含HMGB1量均明顯低于對照組,差異有統計學意義(t=2.783,P=0.012;t=2.509,P=0.021); 72 h時模型組HMGB1錶達量卻明顯高于對照組,差異具有統計學意義(t=4.112,P=0.006).模型組HMGB1蛋白錶達量于造模後12h、24 h、72 h呈現逐漸增高趨勢. 結論 Sombati癲癇細胞模型中HMGB1的錶達呈時間依賴性,隨時間延長錶達增加,可能在癲癇的病理生理中起重要作用.
목적 연구Sombati전간세포모형중고천이솔족단백1(HMGB1)적변화규률. 방법 신생SD유서단두후신속둔성분리출쌍측해마,취해마신경원체외배양지제9천,장신경원분위모형조(무미세포외액처리)화대조조(정상세포외액처리),응용Wester blotting기술검측해마신경원재접수상응처리12、24、72 h후HMGB1단백적표체변화정황. 결과 Wester blotting현시,재신경원접수상응처리후12、24 h,모형조세포소함HMGB1량균명현저우대조조,차이유통계학의의(t=2.783,P=0.012;t=2.509,P=0.021); 72 h시모형조HMGB1표체량각명현고우대조조,차이구유통계학의의(t=4.112,P=0.006).모형조HMGB1단백표체량우조모후12h、24 h、72 h정현축점증고추세. 결론 Sombati전간세포모형중HMGB1적표체정시간의뢰성,수시간연장표체증가,가능재전간적병리생리중기중요작용.
Objective To investigate the expression changes of high-mobility group box-1 (HMGB1) in Sombati's cell models.Methods Hippocampal neurons were collected from bilateral hippocampus in SD suckling mice; they were divided into experimental group (cultured with Mg2+-free extracellular fluid) and control group (cultured in normal Mg2+ levels extracellular fluid).The protein expressions of HMGB1 in the neurons of these two groups were determined at the time points of 12,24and 72 h after treatmnent by Western blotting.Results Western blotting indicated that the expression of HMGB1 in the experimental group was significantly lower than that in the control group at the time points of 12,24 h after treatment (t=2.783,P=0.012; t=2.509,P=0.021); but at the time point of 72 h,that in the experimental group was significantly higher (t=4.112,P=0.006).In addition,from time points of 12 h (0.248±0.118),24 h (0.333±0.216) to the time point of 72 h (0.857±0.253),the expression of HMGB 1 showed upward trend.Conclusion The expressions of HMGB1 show time dependence in the epilepsy of Sombati's cell models,and HMGB 1 may plays an important role in the pathophysiology of epilepsy.
