中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2014年
9期
905-908
,共4页
李三亮%闫军美%李春玲%马丹丹%陈华永
李三亮%閆軍美%李春玲%馬丹丹%陳華永
리삼량%염군미%리춘령%마단단%진화영
丙泊酚%颈内动脉%麻醉%脑%脑电双频指数
丙泊酚%頸內動脈%痳醉%腦%腦電雙頻指數
병박분%경내동맥%마취%뇌%뇌전쌍빈지수
Propofol%Internal carotid artery%Anesthesia%Cerebrum%Bispectral index
目的 观察丙泊酚经大鼠颈内动脉泵注对脑灌注区皮层组织的影响,探讨丙泊酚颈内动脉麻醉的安全性. 方法 SD大鼠40只按随机数字表法分为右股静脉给药组和右颈内动脉给药组(每组各20只),麻醉诱导后分别通过右股静脉或右颈内动脉留置针泵注丙泊酚30 mg/(kg·h),持续3h.停药后4h处死大鼠并取右侧大脑皮层,HE染色、透视电镜观察大鼠右侧大脑皮层组织的形态学和超微结构改变;实时PCR(RT-PCR)检测皮层B细胞淋巴瘤/白血病-2(bcl-2)、bcl-2相关X蛋白(Bax) mRNA的表达. 结果 右股静脉给药组与右颈内动脉给药组大鼠皮层神经元、神经胶质细胞、血管内膜结构均正常,未见急性损伤现象;电镜显示大鼠皮层的神经元细胞核、细胞器、毛细血管内膜结构均正常,未见明显变化;RT-PCR结果显示2组大鼠皮层bcl-2、Bax mRNA的表达比较差异均无统计学意义(P>0.05). 结论 丙泊酚经大鼠颈内动脉泵注对脑灌注区皮层组织的影响不显著.
目的 觀察丙泊酚經大鼠頸內動脈泵註對腦灌註區皮層組織的影響,探討丙泊酚頸內動脈痳醉的安全性. 方法 SD大鼠40隻按隨機數字錶法分為右股靜脈給藥組和右頸內動脈給藥組(每組各20隻),痳醉誘導後分彆通過右股靜脈或右頸內動脈留置針泵註丙泊酚30 mg/(kg·h),持續3h.停藥後4h處死大鼠併取右側大腦皮層,HE染色、透視電鏡觀察大鼠右側大腦皮層組織的形態學和超微結構改變;實時PCR(RT-PCR)檢測皮層B細胞淋巴瘤/白血病-2(bcl-2)、bcl-2相關X蛋白(Bax) mRNA的錶達. 結果 右股靜脈給藥組與右頸內動脈給藥組大鼠皮層神經元、神經膠質細胞、血管內膜結構均正常,未見急性損傷現象;電鏡顯示大鼠皮層的神經元細胞覈、細胞器、毛細血管內膜結構均正常,未見明顯變化;RT-PCR結果顯示2組大鼠皮層bcl-2、Bax mRNA的錶達比較差異均無統計學意義(P>0.05). 結論 丙泊酚經大鼠頸內動脈泵註對腦灌註區皮層組織的影響不顯著.
목적 관찰병박분경대서경내동맥빙주대뇌관주구피층조직적영향,탐토병박분경내동맥마취적안전성. 방법 SD대서40지안수궤수자표법분위우고정맥급약조화우경내동맥급약조(매조각20지),마취유도후분별통과우고정맥혹우경내동맥류치침빙주병박분30 mg/(kg·h),지속3h.정약후4h처사대서병취우측대뇌피층,HE염색、투시전경관찰대서우측대뇌피층조직적형태학화초미결구개변;실시PCR(RT-PCR)검측피층B세포림파류/백혈병-2(bcl-2)、bcl-2상관X단백(Bax) mRNA적표체. 결과 우고정맥급약조여우경내동맥급약조대서피층신경원、신경효질세포、혈관내막결구균정상,미견급성손상현상;전경현시대서피층적신경원세포핵、세포기、모세혈관내막결구균정상,미견명현변화;RT-PCR결과현시2조대서피층bcl-2、Bax mRNA적표체비교차이균무통계학의의(P>0.05). 결론 병박분경대서경내동맥빙주대뇌관주구피층조직적영향불현저.
Objective To explore the security of propofol used in internal carotid artery anesthesia by observing the effect of internal carotid artery infusion of propofol on neurovascular tissue of cerebral infusion areas in rats.Methods A total of 40 SD rats were randomly divided into a V group and an A group (n=20); rats in the V group were given treatment of right femoral vein infusion of 30 mg/ (kg· h) propofol,and those in the A group were given treatment of right internal carotid artery infusion of 30 mg/(kg· h) propofol for 3 h.Rats were sacrificed 4 h after propofol withdrawal.HE staining and transmission electron microscopy were employed to detect the morphological and ultrastructural changes in the right cerebral cortex tissues; and semi-quantitative real time PCR was used to observe the mRNA expressions of bcl-2 and Bax.Results No obvious acute injury was noted in both groups,and the morphology of cortical neurons,neuralgia cells and vascular structures was normal in both groups.And the neuronal nuclei,organelles and capillary endomembrane of both groups were normal,without obvious changes.No significant difference in mRNA expressions ofbcl-2 and Bax was noted between the two groups (P>0.05).Conclusion It is insignificant in the effects of internal carotid artery infusion of propofol on neurovascular tissues of cerebral infusion areas in rats.