中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2014年
9期
929-933
,共5页
神经胶质瘤%血管生成拟态%Ki-67%预后
神經膠質瘤%血管生成擬態%Ki-67%預後
신경효질류%혈관생성의태%Ki-67%예후
Gliomas%Vasculogenic mimicry%Ki-67%Prognosis
目的 探讨血管生成拟态(VM)和Ki-67在脑胶质瘤组织中的表达及临床意义. 方法 收集存档于南方医科大学珠江医院病理科的自2008年1月至2012年12月间诊断为脑胶质瘤的石蜡标本63例,采用过碘酸-雪夫氏反应和CD34双重染色方法检测胶质瘤组织中VM形成情况,免疫组化SP法检测Ki-67表达情况,进一步结合随访完整的临床病理及预后资料进行相关性分析. 结果 63例胶质瘤患者组织中存在VM者13例(20.63％);高级别脑胶质瘤组织中VM发生率(WHOⅢ:19％;WHOⅣ:33.3％)显著高于低级别患者(WHOⅡ:6.7％),VM阳性患者复发率(28.6％)显著高于VM阴性患者(4％),差异均有统计学意义(P＜0.05).VM与Ki-67的表达呈正相关关系(r=0.531,P=0.000).Kaplan-Meier分析显示VM阳性患者无进展生存率和术后总生存率均低于VM阴性患者,差异有统计学意义(P＜0.05).COX比例风险模型多因素分析显示VM是影响胶质瘤患者预后的独立危险因素(=0.020). 结论 脑胶质瘤组织中存在VM,联合检测胶质瘤中VM、Ki-67的表达情况有助于评估肿瘤复发危险性,同时可作为判断胶质瘤患者预后的指标.
目的 探討血管生成擬態(VM)和Ki-67在腦膠質瘤組織中的錶達及臨床意義. 方法 收集存檔于南方醫科大學珠江醫院病理科的自2008年1月至2012年12月間診斷為腦膠質瘤的石蠟標本63例,採用過碘痠-雪伕氏反應和CD34雙重染色方法檢測膠質瘤組織中VM形成情況,免疫組化SP法檢測Ki-67錶達情況,進一步結閤隨訪完整的臨床病理及預後資料進行相關性分析. 結果 63例膠質瘤患者組織中存在VM者13例(20.63％);高級彆腦膠質瘤組織中VM髮生率(WHOⅢ:19％;WHOⅣ:33.3％)顯著高于低級彆患者(WHOⅡ:6.7％),VM暘性患者複髮率(28.6％)顯著高于VM陰性患者(4％),差異均有統計學意義(P＜0.05).VM與Ki-67的錶達呈正相關關繫(r=0.531,P=0.000).Kaplan-Meier分析顯示VM暘性患者無進展生存率和術後總生存率均低于VM陰性患者,差異有統計學意義(P＜0.05).COX比例風險模型多因素分析顯示VM是影響膠質瘤患者預後的獨立危險因素(=0.020). 結論 腦膠質瘤組織中存在VM,聯閤檢測膠質瘤中VM、Ki-67的錶達情況有助于評估腫瘤複髮危險性,同時可作為判斷膠質瘤患者預後的指標.
목적 탐토혈관생성의태(VM)화Ki-67재뇌효질류조직중적표체급림상의의. 방법 수집존당우남방의과대학주강의원병이과적자2008년1월지2012년12월간진단위뇌효질류적석사표본63례,채용과전산-설부씨반응화CD34쌍중염색방법검측효질류조직중VM형성정황,면역조화SP법검측Ki-67표체정황,진일보결합수방완정적림상병리급예후자료진행상관성분석. 결과 63례효질류환자조직중존재VM자13례(20.63％);고급별뇌효질류조직중VM발생솔(WHOⅢ:19％;WHOⅣ:33.3％)현저고우저급별환자(WHOⅡ:6.7％),VM양성환자복발솔(28.6％)현저고우VM음성환자(4％),차이균유통계학의의(P＜0.05).VM여Ki-67적표체정정상관관계(r=0.531,P=0.000).Kaplan-Meier분석현시VM양성환자무진전생존솔화술후총생존솔균저우VM음성환자,차이유통계학의의(P＜0.05).COX비례풍험모형다인소분석현시VM시영향효질류환자예후적독립위험인소(=0.020). 결론 뇌효질류조직중존재VM,연합검측효질류중VM、Ki-67적표체정황유조우평고종류복발위험성,동시가작위판단효질류환자예후적지표.
Objective To investigate the clinical significances ofvasculogenic mimicry (VM) and Ki-67 expression in human gliomas.Methods Sixty-three specimens of human gliomas with adequate clinical and pathological data,collected in our hospital from January 2008 to December 2012,were chosen.VM in the glioma samples was determined by dual staining of periodic acid-Schiff (PAS) and endothelial marker CD34,and Ki-67 protein expressions by SP immunohistochemical staining.The association between VM and clinical characteristics of the patients were analyzed.Results VM channels were found in 13 specimens of human gliomas (20.63％),and it was correlated with tumor grade and tumor recurrence (P＜0.05):high incidence of VM in high-grade gliomas (WHO Ⅲ,19％; WHO Ⅳ,33.3％) was noted as compared with that of lower-grade gliomas (WHO Ⅱ,6.7％); patients with VM were more prone to tumor recurrence (28.6％) as compared with those without VM (4％).A positive relationship between Ki-67 expression and VM was revealed (r=0.531,P=0.000).Kaplan-Meier survival analysis showed that both progression-free survival and overall survival of VM-positive group were significantly shorter than those of VM-negative group (P＜0.05).Cox proportional hazards model analysis indicated that VM was an independent prognostic factor of human gliomas (P=0.020).Conclusion The presence of VM in human gliomas tissues is proved; combined detection of VM and Ki-67 expression can contribute to evaluate the risk of tumor recurrence,and simultaneously be some new indicator of predicting the prognosis of human gliomas.
