CAJ | 학술논문

目的观察胶质瘤中miR200b和SOX2的表达情况,分析miR200b和SOX2与胶质瘤患者预后的关系及其相互关系.方法选取河南省人民医院神经外科自2001年1月至2005年12月手术切除并经病理证实的人脑胶质瘤标本123例,另取23例因颅脑外伤行颅内减压术的正常脑组织作为对照,实时定量PCR(qRT-PCR)检测标本miR200b的表达,免疫组织化学染色检测胶质瘤SOX2的表达;统计分析不同级别胶质瘤miR200b与SOX2表达的关系、胶质瘤患者临床参数与SOX2、miR-200b表达的关系,Cox比例风险回归模型分析miR-200b、SOX2的表达对患者生存时间的影响并比较miR-200b、SOX2表达不同患者的生存曲线.结果胶质瘤的病理分级越高,miR-200b的表达越低,在Ⅲ级及Ⅳ级胶质瘤中,SOX2阳性表达组胶质瘤miR-200b的表达均低于SOX2阴性表达组,差异有统计学意义(P＜0.05).不同病理分级胶质瘤miR-200b高表达率、SOX2阳性表达率比较差异有统计学意义(P＜0.05).Cox比例风险回归模型显示miR-200b、SOX2是胶质瘤患者预后的独立危险因素.在Ⅲ、Ⅳ级胶质瘤患者中,miR-200b高表达患者的5年生存率高于miR-200b低表达患者,SOX2阴性表达患者的5年生存率高于SOX2阳性表达患者,差异有统计学意义(P＜0.05).结论 miR200b和SOX2的表达仅仅在分化程度差的Ⅲ、Ⅳ级胶质瘤组织中有差异,对患者的生存时间有影响.
목적 관찰효질류중miR200b화SOX2적표체정황,분석miR200b화SOX2여효질류환자예후적관계급기상호관계.방법 선취하남성인민의원신경외과자2001년1월지2005년12월수술절제병경병리증실적인뇌효질류표본123례,령취23례인로뇌외상행로내감압술적정상뇌조직작위대조,실시정량PCR(qRT-PCR)검측표본miR200b적표체,면역조직화학염색검측효질류SOX2적표체;통계분석불동급별효질류miR200b여SOX2표체적관계、효질류환자림상삼수여SOX2、miR-200b표체적관계,Cox비례풍험회귀모형분석miR-200b、SOX2적표체대환자생존시간적영향병비교miR-200b、SOX2표체불동환자적생존곡선.결과 효질류적병리분급월고,miR-200b적표체월저,재Ⅲ급급Ⅳ급효질류중,SOX2양성표체조효질류miR-200b적표체균저우SOX2음성표체조,차이유통계학의의(P＜0.05).불동병리분급효질류miR-200b고표체솔、SOX2양성표체솔비교차이유통계학의의(P＜0.05).Cox비례풍험회귀모형현시miR-200b、SOX2시효질류환자예후적독립위험인소.재Ⅲ、Ⅳ급효질류환자중,miR-200b고표체환자적5년생존솔고우miR-200b저표체환자,SOX2음성표체환자적5년생존솔고우SOX2양성표체환자,차이유통계학의의(P＜0.05).결론 miR200b화SOX2적표체부부재분화정도차적Ⅲ、Ⅳ급효질류조직중유차이,대환자적생존시간유영향.
Objective To analyze the relation between SOX2 and miR-200b and their influences in clinical pathology and prognosis of gliomas patients.Methods One hundred and twenty-three human glioma specimens,collected in our hospital from January 2001 to December 2005 and conformed by pathology,were chosen in our study; other 23 healthy brain tissues collected during intracranial decompression were used as controls.Real-time quantitative PCR (qRT-PCR) was employed to detect the miR200b expression; immunohistochemistry was used to detect the SOX2 expression.Relations between SOX2 and miR-200b in different grades of gliomas were analyzed; the correlations of clinical parameters with SOX2 and miR-200b expressions were analyzed; Cox proportional hazards regression model was used to analyze the influences of SOX2 and miR-200b expressions in survival times of the patients,and the survival curves of patients with different SOX2 and miR-200b expressions were compared.Results The higher the pathology grade,the lower the miR-200b expression; gliomas of grade Ⅲ and Ⅳ had SOX2 positive expression enjoyed significantly lower miR-200b expression than those had SOX2 negative expression (P＜0.05).The SOX2 and miR-200b expressions in gliomas of different pathology grades were significantly different (P＜0.05).Cox proportional hazards regression model indicated that miR-200b and SOX2 were the independent risk factors for prognosis of gliomas.In patients of grade Ⅲ and grade Ⅳ gliomas,significantly higher 5 years survival rate in patients with high miR200b expression was noted as compared with that with low miR200b expression (P＜0.05),and significantly higher 5 years survival rate in patients with negative SOX2 expression was noted as compared with those with positive SOX2 expression (P＜0.05).Conclusion The miR-200b and SOX2 expressions show difference only in poor differentiated tissues of grade Ⅲ and Ⅳ gliomas; the two have influence in the survival time.