中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2012年
6期
423-427
,共5页
烧伤,电%血管内皮生长因子类%毛细血管通透性
燒傷,電%血管內皮生長因子類%毛細血管通透性
소상,전%혈관내피생장인자류%모세혈관통투성
Burns,electric%Vascular endothelial growth factors%Capillary permeability
目的 观察电烧伤大鼠血清和创面组织中血管内皮生长因子(VEGF)的表达变化,探讨其在电烧伤病变中的作用机制. 方法 按随机数字表法将64只SD大鼠分为正常对照组(8只)和电烧伤组(56只).于伤后6h和1、3、7、14、21、28 d,每时相点处死8只电烧伤组大鼠,留取创面肌肉组织和血清样本.HE染色观察创面组织病理学改变,双抗体夹心ELISA法检测血清VEGF含量,蛋白质印迹法检测创面VEGF蛋白的表达量,免疫组织化学法检测创面VEGF的表达定位,计算微血管密度(MVD)值.对MVD值与VEGF表达强度行相关性分析.取未经任何处理的正常对照组大鼠腿部腹侧肌肉组织及血清进行上述指标检测.对数据进行单因素方差分析与Spearman等级相关分析,两两比较LSD-t检验. 结果 (1)电烧伤组大鼠伤后6h及1d,创面组织肌纤维断裂,大量炎性细胞浸润,组织水肿明显；伤后3d有新生血管生成；7d时肉芽组织及新生血管数量明显增多.(2)电烧伤组大鼠伤后6h及1、14 d,血清VEGF含量分别为(43±11)、(44±11)、(74±27) pg/mL,明显高于正常对照组的(15±9)pg/mL(t值为4.001～5.724,P值均小于0.01).(3)与正常对照组肌肉组织VEGF蛋白表达量(0.21±0.09)比较,电烧伤组各时相点大鼠创面组织VEGF蛋白表达量均升高,伤后7d达峰值,为0.63±0.13(t =4.965,P＜0.05).(4)电烧伤组早期炎性细胞VEGF表达强阳性,后期以新生血管内皮细胞阳性表达为主.(5)电烧伤组伤后3、7、14、21、28 d,创面组织MVD值和VEGF表达强度呈显著正相关(rs=0.834,P＜0.01). 结论 电烧伤后不同阶段,VEGF在大鼠创面组织及血清中的表达各不相同,其改变与伤后体液渗出及创面愈合过程密切相关.
目的 觀察電燒傷大鼠血清和創麵組織中血管內皮生長因子(VEGF)的錶達變化,探討其在電燒傷病變中的作用機製. 方法 按隨機數字錶法將64隻SD大鼠分為正常對照組(8隻)和電燒傷組(56隻).于傷後6h和1、3、7、14、21、28 d,每時相點處死8隻電燒傷組大鼠,留取創麵肌肉組織和血清樣本.HE染色觀察創麵組織病理學改變,雙抗體夾心ELISA法檢測血清VEGF含量,蛋白質印跡法檢測創麵VEGF蛋白的錶達量,免疫組織化學法檢測創麵VEGF的錶達定位,計算微血管密度(MVD)值.對MVD值與VEGF錶達彊度行相關性分析.取未經任何處理的正常對照組大鼠腿部腹側肌肉組織及血清進行上述指標檢測.對數據進行單因素方差分析與Spearman等級相關分析,兩兩比較LSD-t檢驗. 結果 (1)電燒傷組大鼠傷後6h及1d,創麵組織肌纖維斷裂,大量炎性細胞浸潤,組織水腫明顯；傷後3d有新生血管生成；7d時肉芽組織及新生血管數量明顯增多.(2)電燒傷組大鼠傷後6h及1、14 d,血清VEGF含量分彆為(43±11)、(44±11)、(74±27) pg/mL,明顯高于正常對照組的(15±9)pg/mL(t值為4.001～5.724,P值均小于0.01).(3)與正常對照組肌肉組織VEGF蛋白錶達量(0.21±0.09)比較,電燒傷組各時相點大鼠創麵組織VEGF蛋白錶達量均升高,傷後7d達峰值,為0.63±0.13(t =4.965,P＜0.05).(4)電燒傷組早期炎性細胞VEGF錶達彊暘性,後期以新生血管內皮細胞暘性錶達為主.(5)電燒傷組傷後3、7、14、21、28 d,創麵組織MVD值和VEGF錶達彊度呈顯著正相關(rs=0.834,P＜0.01). 結論 電燒傷後不同階段,VEGF在大鼠創麵組織及血清中的錶達各不相同,其改變與傷後體液滲齣及創麵愈閤過程密切相關.
목적 관찰전소상대서혈청화창면조직중혈관내피생장인자(VEGF)적표체변화,탐토기재전소상병변중적작용궤제. 방법 안수궤수자표법장64지SD대서분위정상대조조(8지)화전소상조(56지).우상후6h화1、3、7、14、21、28 d,매시상점처사8지전소상조대서,류취창면기육조직화혈청양본.HE염색관찰창면조직병이학개변,쌍항체협심ELISA법검측혈청VEGF함량,단백질인적법검측창면VEGF단백적표체량,면역조직화학법검측창면VEGF적표체정위,계산미혈관밀도(MVD)치.대MVD치여VEGF표체강도행상관성분석.취미경임하처리적정상대조조대서퇴부복측기육조직급혈청진행상술지표검측.대수거진행단인소방차분석여Spearman등급상관분석,량량비교LSD-t검험. 결과 (1)전소상조대서상후6h급1d,창면조직기섬유단렬,대량염성세포침윤,조직수종명현；상후3d유신생혈관생성；7d시육아조직급신생혈관수량명현증다.(2)전소상조대서상후6h급1、14 d,혈청VEGF함량분별위(43±11)、(44±11)、(74±27) pg/mL,명현고우정상대조조적(15±9)pg/mL(t치위4.001～5.724,P치균소우0.01).(3)여정상대조조기육조직VEGF단백표체량(0.21±0.09)비교,전소상조각시상점대서창면조직VEGF단백표체량균승고,상후7d체봉치,위0.63±0.13(t =4.965,P＜0.05).(4)전소상조조기염성세포VEGF표체강양성,후기이신생혈관내피세포양성표체위주.(5)전소상조상후3、7、14、21、28 d,창면조직MVD치화VEGF표체강도정현저정상관(rs=0.834,P＜0.01). 결론 전소상후불동계단,VEGF재대서창면조직급혈청중적표체각불상동,기개변여상후체액삼출급창면유합과정밀절상관.
Objective To observe the change in expression of vascular endothelial growth factor (VEGF) in serum and wound tissue of rats with electrical burn (EB),and to explore its regulation mechanism in the pathological changes of EB.Methods Sixty-four SD rats were divided into normal control group (n =8) and EB group (n =56) according to the random number table.Eight rats in EB group were sacrificed at post injury hour (PIH) 6 and on post injury day (PID) 1,3,7,14,21,and 28,to collect wound muscle tissue and serum samples.Histopathological changes in wound tissue were observed with HE staining.The serum content of VEGF was determined with double-antibody sandwich enzyme-linked immunosorbent assay.The expression level of VEGF in wound tissue was determined with Western blotting.VEGF expression intensity in wound tissue was observed with immunohistochemical staining.The microvessel density (MVD) was calculated.The correlation between VEGF expression intensity and MVD was analyzed.Muscle tissue of calf and serum of the rats in normal control group without any treatment were collected for above-mentioned observations and determinations.Data were processed with one-way analysis of variance and Spearman hierarchy correlation analysis,and LSD-t test was applied for paired comparison.Results (1)In EB group,breakage of muscle fiber,heavy infiltration of inflammatory cells,and obvious tissue edema were observed at PIH 6 and on PID 1 ; new vessels were observed on PID 3 ; amount of granulation tissue and number of new vessels were found to be increased on PID 7.(2) In EB group,the serum level of VEGF was (43 ± 11) pg/mL at PIH 6,(44 ± 11) pg/mL on PID 1,and (74 ± 27) pg/mL on PID 14,which were all significantly higher than that in normal control group [(15 ± 9) pg/mL,with t values from 4.001 to 5.724,P values all below 0.01].(3) The protein expression level of VEGF of wound tissue in EB group was higher than that in normal control group (0.21 ± 0.09) at each time point.The protein expression level of VEGF in EB group peaked on PID 7 (0.63 ±0.13,t =4.965,P ＜0.05).(4) In EB group,strongly positive expression of VEGF was observed in inflammatory cells at early stage and in new vascular endothelial cells at late stage.(5) The expression intensity of VEGF was positively correlated with MVD in wound tissue on PID 3,7,14,21,and 28 in EB group (rs =0.834,P ＜ 0.01).Conclusions Different expression levels of VEGF were observed in serum and wound tissue of rats at various stages after EB,and they were closely correlated with different stages of fluid exudation and wound healing process after EB.
