CAJ | 학술논문

目的了解高压电烧伤对大鼠血小板、白细胞聚集黏附的影响,探讨己酮可可碱(PTX)的干预作用. 方法将180只SD大鼠按随机数字表法分为对照组、电伤组和治疗组,每组60只.(1)3组致伤前15 min各取10只大鼠,用布莱德多项投影显微镜系统观测肠系膜微静脉白细胞黏附数；用激光多普勒组织灌流图像仪检测胸部皮肤微循环灌流量；心脏采血分离血清,用双抗体夹心ELISA法检测血清中血小板活化因子(PAF)、血栓烷B2(TXB2)、前列环素(PGI2)、P选择素、E选择素及L选择素含量,并计算TXB2/PGI2比值.(2)电伤组和治疗组余下各50只大鼠用调压器及实验变压器制成高压电烧伤模型(左前肢为电流入口,右后肢为电流出口),对照组余下50只大鼠接相同装置但不通电致假伤.伤后2 min内,经腹腔注射2 mL生理盐水(对照组、电伤组)或者2 mL浓度为50 mg/mL PTX注射液(治疗组).伤后5 min和1、2、4、8h于每组各取10只大鼠,同前检测白细胞黏附数和微循环灌流量、血清血小板及白细胞相关因子水平,计算相关比值.对数据行两因素析因设计的方差分析和LSD检验. 结果电伤组大鼠PAF、TXB2、PGI2、P选择素、E选择素、L选择素含量以及TXB2/PGI2比值和白细胞黏附数总体检测值大于对照组；微循环灌流量小于对照组,处理因素主效应F值为854.20～8156.52,P值均小于0.01.治疗组大鼠PAF、TXB2、P选择素、E选择素、L选择素含量以及TXB2/PGI2比值和白细胞黏附数总体检测值小于电伤组；PGI2含量、微循环灌流量大于电伤组,处理因素主效应F值为33.18～1033.99,P值均小于0.01.统计学结果仅允许在电伤组、治疗组组内进行比较.除治疗组伤后5 min TXB2/PGI2比值、电伤组和治疗组伤后5 minE选择素含量外,电伤组及治疗组大鼠伤后各时相点PAF、TXB2、PGI2、P选择素、E选择素、L选择素含量及TXB2/PGI2比值和白细胞黏附数显著大于伤前15 min；微循环灌流量显著小于伤前15 min(P值均小于0.001).电伤组PAF、TXB2、E选择素含量及TXB2/PGI2比值于伤后4h达峰值,分别为(9.3±0.9) ng/mL、(14.31±0.65)nmol/mL、(271.2±18.4) ng/mL、4.62±0.26；PGI2、P选择素含量及每100微米微静脉白细胞黏附数伤后8h达峰值,分别为(3.98±0.24) nmol/mL、(514±24) ng/mL、(25.50±4.14)个；L选择素含量于伤后2h达峰值,为(876±54) ng/mL；微循环灌流量以伤后5 min最小,为(1.17±0.10)V. 结论高压电烧伤引起大鼠PAF、TXB2、PGI2、P选择素、E选择素、L选择索含量及TXB2/PGI2比值升高,白细胞黏附数增加及微循环灌流量下降.PTX通过增加PGI2的含量,降低上述其他因子的含量而抑制血小板及白细胞聚集黏附,从而改善电烧伤后微循环障碍. 目的瞭解高壓電燒傷對大鼠血小闆、白細胞聚集黏附的影響,探討己酮可可堿(PTX)的榦預作用. 方法將180隻SD大鼠按隨機數字錶法分為對照組、電傷組和治療組,每組60隻.(1)3組緻傷前15 min各取10隻大鼠,用佈萊德多項投影顯微鏡繫統觀測腸繫膜微靜脈白細胞黏附數；用激光多普勒組織灌流圖像儀檢測胸部皮膚微循環灌流量；心髒採血分離血清,用雙抗體夾心ELISA法檢測血清中血小闆活化因子(PAF)、血栓烷B2(TXB2)、前列環素(PGI2)、P選擇素、E選擇素及L選擇素含量,併計算TXB2/PGI2比值.(2)電傷組和治療組餘下各50隻大鼠用調壓器及實驗變壓器製成高壓電燒傷模型(左前肢為電流入口,右後肢為電流齣口),對照組餘下50隻大鼠接相同裝置但不通電緻假傷.傷後2 min內,經腹腔註射2 mL生理鹽水(對照組、電傷組)或者2 mL濃度為50 mg/mL PTX註射液(治療組).傷後5 min和1、2、4、8h于每組各取10隻大鼠,同前檢測白細胞黏附數和微循環灌流量、血清血小闆及白細胞相關因子水平,計算相關比值.對數據行兩因素析因設計的方差分析和LSD檢驗. 結果電傷組大鼠PAF、TXB2、PGI2、P選擇素、E選擇素、L選擇素含量以及TXB2/PGI2比值和白細胞黏附數總體檢測值大于對照組；微循環灌流量小于對照組,處理因素主效應F值為854.20～8156.52,P值均小于0.01.治療組大鼠PAF、TXB2、P選擇素、E選擇素、L選擇素含量以及TXB2/PGI2比值和白細胞黏附數總體檢測值小于電傷組；PGI2含量、微循環灌流量大于電傷組,處理因素主效應F值為33.18～1033.99,P值均小于0.01.統計學結果僅允許在電傷組、治療組組內進行比較.除治療組傷後5 min TXB2/PGI2比值、電傷組和治療組傷後5 minE選擇素含量外,電傷組及治療組大鼠傷後各時相點PAF、TXB2、PGI2、P選擇素、E選擇素、L選擇素含量及TXB2/PGI2比值和白細胞黏附數顯著大于傷前15 min；微循環灌流量顯著小于傷前15 min(P值均小于0.001).電傷組PAF、TXB2、E選擇素含量及TXB2/PGI2比值于傷後4h達峰值,分彆為(9.3±0.9) ng/mL、(14.31±0.65)nmol/mL、(271.2±18.4) ng/mL、4.62±0.26；PGI2、P選擇素含量及每100微米微靜脈白細胞黏附數傷後8h達峰值,分彆為(3.98±0.24) nmol/mL、(514±24) ng/mL、(25.50±4.14)箇；L選擇素含量于傷後2h達峰值,為(876±54) ng/mL；微循環灌流量以傷後5 min最小,為(1.17±0.10)V. 結論高壓電燒傷引起大鼠PAF、TXB2、PGI2、P選擇素、E選擇素、L選擇索含量及TXB2/PGI2比值升高,白細胞黏附數增加及微循環灌流量下降.PTX通過增加PGI2的含量,降低上述其他因子的含量而抑製血小闆及白細胞聚集黏附,從而改善電燒傷後微循環障礙.
목적 료해고압전소상대대서혈소판、백세포취집점부적영향,탐토기동가가감(PTX)적간예작용. 방법 장180지SD대서안수궤수자표법분위대조조、전상조화치료조,매조60지.(1)3조치상전15 min각취10지대서,용포래덕다항투영현미경계통관측장계막미정맥백세포점부수；용격광다보륵조직관류도상의검측흉부피부미순배관류량；심장채혈분리혈청,용쌍항체협심ELISA법검측혈청중혈소판활화인자(PAF)、혈전완B2(TXB2)、전렬배소(PGI2)、P선택소、E선택소급L선택소함량,병계산TXB2/PGI2비치.(2)전상조화치료조여하각50지대서용조압기급실험변압기제성고압전소상모형(좌전지위전류입구,우후지위전류출구),대조조여하50지대서접상동장치단불통전치가상.상후2 min내,경복강주사2 mL생리염수(대조조、전상조)혹자2 mL농도위50 mg/mL PTX주사액(치료조).상후5 min화1、2、4、8h우매조각취10지대서,동전검측백세포점부수화미순배관류량、혈청혈소판급백세포상관인자수평,계산상관비치.대수거행량인소석인설계적방차분석화LSD검험. 결과 전상조대서PAF、TXB2、PGI2、P선택소、E선택소、L선택소함량이급TXB2/PGI2비치화백세포점부수총체검측치대우대조조；미순배관류량소우대조조,처리인소주효응F치위854.20～8156.52,P치균소우0.01.치료조대서PAF、TXB2、P선택소、E선택소、L선택소함량이급TXB2/PGI2비치화백세포점부수총체검측치소우전상조；PGI2함량、미순배관류량대우전상조,처리인소주효응F치위33.18～1033.99,P치균소우0.01.통계학결과부윤허재전상조、치료조조내진행비교.제치료조상후5 min TXB2/PGI2비치、전상조화치료조상후5 minE선택소함량외,전상조급치료조대서상후각시상점PAF、TXB2、PGI2、P선택소、E선택소、L선택소함량급TXB2/PGI2비치화백세포점부수현저대우상전15 min；미순배관류량현저소우상전15 min(P치균소우0.001).전상조PAF、TXB2、E선택소함량급TXB2/PGI2비치우상후4h체봉치,분별위(9.3±0.9) ng/mL、(14.31±0.65)nmol/mL、(271.2±18.4) ng/mL、4.62±0.26；PGI2、P선택소함량급매100미미미정맥백세포점부수상후8h체봉치,분별위(3.98±0.24) nmol/mL、(514±24) ng/mL、(25.50±4.14)개；L선택소함량우상후2h체봉치,위(876±54) ng/mL；미순배관류량이상후5 min최소,위(1.17±0.10)V. 결론 고압전소상인기대서PAF、TXB2、PGI2、P선택소、E선택소、L선택색함량급TXB2/PGI2비치승고,백세포점부수증가급미순배관류량하강.PTX통과증가PGI2적함량,강저상술기타인자적함량이억제혈소판급백세포취집점부,종이개선전소상후미순배장애.
Objective To investigate the influence of high-voltage electrical bum (HEB) on the aggregation and adhesion of platelet and leukocyte in rats and the interventional effect of pentoxifylline (PTX).Methods One hundred and eighty SD rats were divided into control,electrical burn (EB),and pentoxifylline treatment (PT) groups according to the random number table,with 60 rats in each group.(1) Ten rats were taken from each group at 15 minutes before injury for the observation of the microcirculatory perfusion of chest skin with Laser Doppler Perfusion Imager (LDPI),and the number of leukocyte adherent to mesenteric venule with Bradford Variable Projection Microscope (BVPM).Serum was collected from heart blood to determine the contents of platelet activating factor (PAF),thromboxane B2 (TXB2),prostacyclin (PGI2),P-selectin,E-selectin and L-selectin by double-antibody sandwich enzyme-linked immunosorbent assay.The ratio of TXB2 to PGI2 was calculated therefrom.(2) Model of HEB was reproduced in the remaining 50 rats of EB group and that of PT group with voltage regulator and experimental transformer (the electrical current applied to the left forelimb and exited from the right hind limb).The remaining 50 rats of control group were sham injured with the same devices without electric current.Within 2 minutes post injury (PIM),rats in control group and EB group were intraperitoneally injected with 2 mL isotonic saline,while rats in PT group were intraperitoneally injected with 2 mL pentoxifylline (50 mg/mL).At PIM 5 and 1,2,4,8 hour (s)post injury (PIH),10 rats of every group were randomly chosen at each time point for the observation of the microcirculatory perfusion of chest skin and the number of leukocytes adherent to mesenteric venule through the same method as used above,and the levels of the related factors of aggregation and adhesion of platelets and leukocytes were determined,and then the relative ratio was calculated.Data were processed with the analysis of variance of factorial design and LSD test.Results The contents of PAF,TXB2,PGI2,P-selectin,E-selectin,L-selectin,and the ratio of TXB2 to PGI2,as well as the number of adhered leukocyte in EB group were higher,while the microcirculatory perfusion value was lower than those of control group,with F values from 854.20 to 8156.52,P values all below 0.01.The microcirculatory perfusion value and PGI2content of PT group were higher,while the contents or number of other indexes were lower than those of EB group,with F values from 33.18 to 1033.99,P values all below 0.01.Only the data within EB group and PT group were comparable.The contents of PAF,TXB2,PGI2,P-selectin,E-selectin,L-selectin,and the ratio of TXB2 to PGI2,as well as the number of adhered leukocyte in EB group and PT group at each time point were significantly higher than those at 15 minutes before injury,while the microcirculation perfusion value was significantly lower than that at 15 minutes before injury (P values all below 0.001),with the exception of the ratio of TXB2 to PGI2 in PT group and E-selectin in EB group and PT group at PIM 5.The contents of PAF,TXB2,and E-selectin and the ratio of TXB2 to PGI2 in EB group peaked at PIH 4,and they were respectively (9.3 ± 0.9) ng/mL,(14.31 ± 0.65) nmol/mL,(271.2 ± 18.4) ng/mL and 4.62 ±0.26.The contents of PGI2 and P-selectin,and the number of adhered leukocyte in EB group peaked at PIH 8,and they were respectively (3.98 ±0.24) nmol/mL,(514 ±24) ng/mL,and (25.50 ±4.14) per 100 μm venule.The content of L-selectin peaked at PIH 2 [(876 ± 54) ng/mL].The microcirculatory perfusion value was lowest at PIM 5 [(1.17 ± 0.10) V].Conclusions HEB can increase the contents of PAF,TXB2,PGI2,P-selectin,E-selectin,L-selectin,the ratio of TXB2 to PGI2,and the number of adhered leukocyte,as well as decrease the skin microcirculatory perfusion value.PTX can inhibit the aggregation and adhesion of platelets and leukocytes through increasing the content of PGI2 and decreasing contents of other factors mentioned above,thus alleviating the microcirculatory dysfunction after HEB.