中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2012年
6期
451-454
,共4页
腐胺%尸胺%炎症因子%坏死组织分解物
腐胺%尸胺%炎癥因子%壞死組織分解物
부알%시알%염증인자%배사조직분해물
Putrescine%Cadaverine%Inflammatory factor%Necrotic tissue decomposition product
目的 探讨外源性腐胺、尸胺对兔外周血炎症因子的影响. 方法 取40只普通级成年新西兰兔,按随机数字表法分成生理盐水组、坏死组织匀浆液(简称匀浆液)组、腐胺组、尸胺组,每组10只.生理盐水组兔腹腔注射生理盐水(1 mL/kg),匀浆液组兔腹腔注射匀浆液(1 mL/kg),腐胺组兔腹腔注射浓度为10 g/L的腐胺(1 mL/kg),尸胺组兔腹腔注射浓度10 g/L的尸胺(1mL/kg).注射前及注射后2、6、12、24、30、36、48、60 h经兔耳中央动脉抽血2 mL,采用ELISA法动态观察血清标本中TNF-α、IL-1、IL-6的含量变化.对数据进行重复测量方差分析,用Cubic模型曲线对各组炎症因子含量变化进行曲线拟合,并进行Spearman相关分析. 结果 (1)各组兔血清炎症因子含量变化:①生理盐水组3种炎症因子含量无明显变化.与注射前比较,匀浆液组、腐胺组及尸胺组注射后大部分时相点3种炎症因子含量呈不同程度升高,与生理盐水组比较差异有统计学意义(F值为3.49～13.58,P值均小于0.05).②腐胺组血清TNF-α、IL-1、IL-6含量分别于注射后2、6、6h明显上升,此趋势与匀浆液组相似,但较尸胺组(均为注射后2h)延迟.③腐胺组血清TNF-α、IL-1、IL-6含量峰值分别为(339±36)、(518±44)、(265.9±33.5) pg/mL,显著低于尸胺组的(476±86)、(539±22)、(309.4±27.1) pg/mL,F值分别为5.11、1.90、5.56,P值均小于0.05.(2)各组兔血清炎症因子含量变化曲线拟合结果:①腐胺组3种炎症因子含量开始上升时间段(注射后3～4h)较尸胺组(注射后2h)延迟,各炎症因子含量达峰值时间段(注射后18～30 h)显著晚于尸胺组(注射后12～30 h),且峰值持续时间段(注射后18～30 h)短于尸胺组(注射后12～30 h).②腐胺组3种炎症因子含量上升时间段和峰值持续时间段均与匀浆液组(分别为注射后3～5、18～ 30 h)较为接近.③相关系数检验分析显示,腐胺组与匀浆液组炎症因子含量变化趋势呈显著相关(rTNF-α=0.933,P＜0.01；rIL-1=0.967,P＜0.01；rIL-6S=0.950,P＜0.01),而尸胺组与匀浆液组仅IL-1、IL-6含量变化趋势有一定相关性(rIL-1 =0.913,P＜0.01；r IL-6 =0.883,P＜0.05). 结论 外源性腐胺和尸胺均可引起机体炎症反应,其中腐胺与匀浆液诱导的机体炎症反应较为相似,提示其在坏死组织分解物诱导的炎症反应中可能起重要作用.
目的 探討外源性腐胺、尸胺對兔外週血炎癥因子的影響. 方法 取40隻普通級成年新西蘭兔,按隨機數字錶法分成生理鹽水組、壞死組織勻漿液(簡稱勻漿液)組、腐胺組、尸胺組,每組10隻.生理鹽水組兔腹腔註射生理鹽水(1 mL/kg),勻漿液組兔腹腔註射勻漿液(1 mL/kg),腐胺組兔腹腔註射濃度為10 g/L的腐胺(1 mL/kg),尸胺組兔腹腔註射濃度10 g/L的尸胺(1mL/kg).註射前及註射後2、6、12、24、30、36、48、60 h經兔耳中央動脈抽血2 mL,採用ELISA法動態觀察血清標本中TNF-α、IL-1、IL-6的含量變化.對數據進行重複測量方差分析,用Cubic模型麯線對各組炎癥因子含量變化進行麯線擬閤,併進行Spearman相關分析. 結果 (1)各組兔血清炎癥因子含量變化:①生理鹽水組3種炎癥因子含量無明顯變化.與註射前比較,勻漿液組、腐胺組及尸胺組註射後大部分時相點3種炎癥因子含量呈不同程度升高,與生理鹽水組比較差異有統計學意義(F值為3.49～13.58,P值均小于0.05).②腐胺組血清TNF-α、IL-1、IL-6含量分彆于註射後2、6、6h明顯上升,此趨勢與勻漿液組相似,但較尸胺組(均為註射後2h)延遲.③腐胺組血清TNF-α、IL-1、IL-6含量峰值分彆為(339±36)、(518±44)、(265.9±33.5) pg/mL,顯著低于尸胺組的(476±86)、(539±22)、(309.4±27.1) pg/mL,F值分彆為5.11、1.90、5.56,P值均小于0.05.(2)各組兔血清炎癥因子含量變化麯線擬閤結果:①腐胺組3種炎癥因子含量開始上升時間段(註射後3～4h)較尸胺組(註射後2h)延遲,各炎癥因子含量達峰值時間段(註射後18～30 h)顯著晚于尸胺組(註射後12～30 h),且峰值持續時間段(註射後18～30 h)短于尸胺組(註射後12～30 h).②腐胺組3種炎癥因子含量上升時間段和峰值持續時間段均與勻漿液組(分彆為註射後3～5、18～ 30 h)較為接近.③相關繫數檢驗分析顯示,腐胺組與勻漿液組炎癥因子含量變化趨勢呈顯著相關(rTNF-α=0.933,P＜0.01；rIL-1=0.967,P＜0.01；rIL-6S=0.950,P＜0.01),而尸胺組與勻漿液組僅IL-1、IL-6含量變化趨勢有一定相關性(rIL-1 =0.913,P＜0.01；r IL-6 =0.883,P＜0.05). 結論 外源性腐胺和尸胺均可引起機體炎癥反應,其中腐胺與勻漿液誘導的機體炎癥反應較為相似,提示其在壞死組織分解物誘導的炎癥反應中可能起重要作用.
목적 탐토외원성부알、시알대토외주혈염증인자적영향. 방법 취40지보통급성년신서란토,안수궤수자표법분성생리염수조、배사조직균장액(간칭균장액)조、부알조、시알조,매조10지.생리염수조토복강주사생리염수(1 mL/kg),균장액조토복강주사균장액(1 mL/kg),부알조토복강주사농도위10 g/L적부알(1 mL/kg),시알조토복강주사농도10 g/L적시알(1mL/kg).주사전급주사후2、6、12、24、30、36、48、60 h경토이중앙동맥추혈2 mL,채용ELISA법동태관찰혈청표본중TNF-α、IL-1、IL-6적함량변화.대수거진행중복측량방차분석,용Cubic모형곡선대각조염증인자함량변화진행곡선의합,병진행Spearman상관분석. 결과 (1)각조토혈청염증인자함량변화:①생리염수조3충염증인자함량무명현변화.여주사전비교,균장액조、부알조급시알조주사후대부분시상점3충염증인자함량정불동정도승고,여생리염수조비교차이유통계학의의(F치위3.49～13.58,P치균소우0.05).②부알조혈청TNF-α、IL-1、IL-6함량분별우주사후2、6、6h명현상승,차추세여균장액조상사,단교시알조(균위주사후2h)연지.③부알조혈청TNF-α、IL-1、IL-6함량봉치분별위(339±36)、(518±44)、(265.9±33.5) pg/mL,현저저우시알조적(476±86)、(539±22)、(309.4±27.1) pg/mL,F치분별위5.11、1.90、5.56,P치균소우0.05.(2)각조토혈청염증인자함량변화곡선의합결과:①부알조3충염증인자함량개시상승시간단(주사후3～4h)교시알조(주사후2h)연지,각염증인자함량체봉치시간단(주사후18～30 h)현저만우시알조(주사후12～30 h),차봉치지속시간단(주사후18～30 h)단우시알조(주사후12～30 h).②부알조3충염증인자함량상승시간단화봉치지속시간단균여균장액조(분별위주사후3～5、18～ 30 h)교위접근.③상관계수검험분석현시,부알조여균장액조염증인자함량변화추세정현저상관(rTNF-α=0.933,P＜0.01；rIL-1=0.967,P＜0.01；rIL-6S=0.950,P＜0.01),이시알조여균장액조부IL-1、IL-6함량변화추세유일정상관성(rIL-1 =0.913,P＜0.01；r IL-6 =0.883,P＜0.05). 결론 외원성부알화시알균가인기궤체염증반응,기중부알여균장액유도적궤체염증반응교위상사,제시기재배사조직분해물유도적염증반응중가능기중요작용.
Objective To explore the influence of exogenous putrescine and cadaverine on pro-inflammatory factors in the peripheral blood of rabbits.Methods Forty ordinary adult New Zealand rabbits were divided into saline,necrotic tissue homogenate (NTH),putrescine,and cadaverine groups according to the random number table,with 10 rabbits in each group.Saline,NTH,10 g/L putrescine,and 10 g/L cadaverine were respectively peritoneally injected into rabbits of corresponding group in the amount of 1 mL/kg.The blood sample in the volume of 2 mL was collected from the central artery of rabbit ears before injection and at 2,6,12,24,30,36,48,60 hours post injection (PIH).Contents of TNF-α,IL-1,and IL-6 in the serum were determined with enzyme-linked immunosorbent assay.Data were processed with repeated measurement data analysis of variance and Spearman correlation analysis,and cubic model curve was applied in curve fitting for the contents of inflammatory factors.Results (1) The serum contents of TNF-α,IL-1,and IL-6 were increased in NTH,putrescine,and cadaverine groups in different degrees at most post injection time points.There was no significant change in the concentrations of the three pro-inflammatory factors in saline group,and they were significantly lower than those of the other three groups at most post injection time points (with F values from 3.49 to 13.58,P values all below 0.05).The serum contents of TNF-α,IL-1,and IL-6 in putrescine group began to increase at PIH 2,6,and 6,which was similar to the trend of NTH group,but the changes were delayed compared with those of cadaverine group(all at PIH 2).The peak values of TNF-α,IL-1,and IL-6 in putrescine group were respectively (339 ± 36),(518 ±44),and (265.9 ± 33.5) pg/mL,which were significantly lower than those of cadaverine group [(476±86),(539±22),and (309.4 ±27.1) pg/mL],with F values respectively 5.11,1.90.and 5.56,P values all below 0.05.(2) The period of time in which contents of TNF-α,IL-1,and IL-6 began to increase (PIH 3-4) and the peaking time of the three pro-inflammatory cytokines (PIH 18-30) in putrescine group appeared later than those of cadaverine group (PIH 2 and 12-30).The duration of peaking time of the three pro-inflammatory cytokines in putrescine group was shorter than that of cadaverine group (PIH 18-30 vs.PIH 12-30).The increasing period and the duration of peaking time of TNF-α,IL-1,and IL-6 in putrescine group were close to those of NTH group (PIH 3-5 and 18-30).The correlation coefficient test analysis showed that the trends of changes in contents of three pro-inflammatory cytokines in putrescine group were significantly correlated with those of NTH group (r TNF-α =0.933,P ＜ 0.01 ; r IL-1 =0.967,P ＜ 0.01 ;r IL-6 =0.950,P ＜0.01).The obvious correlation between cadaverine group and NTH group was only found in the contents of IL-1 and IL-6 (rIL-1 =0.913,P ＜0.01; rIL-6 =0.883,P ＜0.05).Conclusions Both exogenous putrescine and cadaverine can cause inflammatory reaction in rabbits.The trend of the inflammatory reaction induced by putrescine was similar with that by NTH,suggesting that putrescine may play a leading role in the inflammatory reaction induced by necrotic tissue decomposition.
