中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2013年
3期
245-248
,共4页
李志清%肖德权%王甲汉%黄跃生
李誌清%肖德權%王甲漢%黃躍生
리지청%초덕권%왕갑한%황약생
烧伤%心肌%水通道蛋白质1%水肿
燒傷%心肌%水通道蛋白質1%水腫
소상%심기%수통도단백질1%수종
Burns%Myocardium%Aquaporin 1%Edema
目的 了解水通道蛋白1(AQP-1)在大鼠烫伤早期心肌组织中的表达变化及其与心肌水肿的关系. 方法 选取36只Wistar大鼠按照随机数字表法分为正常对照组(6只,不行任何处理)、烫伤组(30只),烫伤组大鼠在麻醉后于其背部造成30％ TBSAⅢ度烫伤,伤后腹腔注射林格平衡盐溶液抗休克.于伤后2、8、12、24、48 h收集烫伤组大鼠左心室心肌组织和血清标本(各时相点取6只大鼠),采用于湿质量法检测心肌组织含水率,免疫组织化学染色观察AQP-1在心肌组织中表达分布,实时定量PCR检测心肌组织AQP-1 mRNA表达,ELISA法测定血清心肌肌钙蛋白I(cTnI)含量.正常对照组大鼠行相同检测.对数据进行单因素方差分析和LSD检验,分别对烫伤组大鼠伤后各时相点AQP-1 mRNA表达量与相应时相点的心肌组织含水率、血清cTnI含量进行相关性分析.结果 与正常对照组大鼠比较,烫伤组大鼠伤后8～48 h心肌含水率均明显增加(P值均小于0.01),其中伤后12 h达峰值[(80.79±0.12)％].免疫组织化学染色显示,AQP-1主要表达于正常对照组和烫伤组大鼠心肌组织的毛细血管内皮细胞和心肌细胞膜,其中烫伤组大鼠伤后2 ～48 h心肌组织中AQP-1表达明显增多.与正常对照组大鼠比较,烫伤组大鼠伤后2～48 h心肌组织AQP-1mRNA表达明显增加(P值均小于0.01),其中伤后12h达峰值(6.2±0.7).与正常对照组大鼠比较,烫伤组大鼠血清cTnI含量伤后2～48 h均明显增加(P值均小于0.01),其中伤后12h达峰值[(5.83 ±0.51) μg/L].烫伤组大鼠心肌组织AQP-1 mRNA表达量与心肌组织含水率、血清cTnI含量均呈显著正相关(r值分别为0.849、0.973,P值均小于0.01). 结论 大鼠烫伤后AQP-1可能参与心肌组织水肿的形成.
目的 瞭解水通道蛋白1(AQP-1)在大鼠燙傷早期心肌組織中的錶達變化及其與心肌水腫的關繫. 方法 選取36隻Wistar大鼠按照隨機數字錶法分為正常對照組(6隻,不行任何處理)、燙傷組(30隻),燙傷組大鼠在痳醉後于其揹部造成30％ TBSAⅢ度燙傷,傷後腹腔註射林格平衡鹽溶液抗休剋.于傷後2、8、12、24、48 h收集燙傷組大鼠左心室心肌組織和血清標本(各時相點取6隻大鼠),採用于濕質量法檢測心肌組織含水率,免疫組織化學染色觀察AQP-1在心肌組織中錶達分佈,實時定量PCR檢測心肌組織AQP-1 mRNA錶達,ELISA法測定血清心肌肌鈣蛋白I(cTnI)含量.正常對照組大鼠行相同檢測.對數據進行單因素方差分析和LSD檢驗,分彆對燙傷組大鼠傷後各時相點AQP-1 mRNA錶達量與相應時相點的心肌組織含水率、血清cTnI含量進行相關性分析.結果 與正常對照組大鼠比較,燙傷組大鼠傷後8～48 h心肌含水率均明顯增加(P值均小于0.01),其中傷後12 h達峰值[(80.79±0.12)％].免疫組織化學染色顯示,AQP-1主要錶達于正常對照組和燙傷組大鼠心肌組織的毛細血管內皮細胞和心肌細胞膜,其中燙傷組大鼠傷後2 ～48 h心肌組織中AQP-1錶達明顯增多.與正常對照組大鼠比較,燙傷組大鼠傷後2～48 h心肌組織AQP-1mRNA錶達明顯增加(P值均小于0.01),其中傷後12h達峰值(6.2±0.7).與正常對照組大鼠比較,燙傷組大鼠血清cTnI含量傷後2～48 h均明顯增加(P值均小于0.01),其中傷後12h達峰值[(5.83 ±0.51) μg/L].燙傷組大鼠心肌組織AQP-1 mRNA錶達量與心肌組織含水率、血清cTnI含量均呈顯著正相關(r值分彆為0.849、0.973,P值均小于0.01). 結論 大鼠燙傷後AQP-1可能參與心肌組織水腫的形成.
목적 료해수통도단백1(AQP-1)재대서탕상조기심기조직중적표체변화급기여심기수종적관계. 방법 선취36지Wistar대서안조수궤수자표법분위정상대조조(6지,불행임하처리)、탕상조(30지),탕상조대서재마취후우기배부조성30％ TBSAⅢ도탕상,상후복강주사림격평형염용액항휴극.우상후2、8、12、24、48 h수집탕상조대서좌심실심기조직화혈청표본(각시상점취6지대서),채용우습질량법검측심기조직함수솔,면역조직화학염색관찰AQP-1재심기조직중표체분포,실시정량PCR검측심기조직AQP-1 mRNA표체,ELISA법측정혈청심기기개단백I(cTnI)함량.정상대조조대서행상동검측.대수거진행단인소방차분석화LSD검험,분별대탕상조대서상후각시상점AQP-1 mRNA표체량여상응시상점적심기조직함수솔、혈청cTnI함량진행상관성분석.결과 여정상대조조대서비교,탕상조대서상후8～48 h심기함수솔균명현증가(P치균소우0.01),기중상후12 h체봉치[(80.79±0.12)％].면역조직화학염색현시,AQP-1주요표체우정상대조조화탕상조대서심기조직적모세혈관내피세포화심기세포막,기중탕상조대서상후2 ～48 h심기조직중AQP-1표체명현증다.여정상대조조대서비교,탕상조대서상후2～48 h심기조직AQP-1mRNA표체명현증가(P치균소우0.01),기중상후12h체봉치(6.2±0.7).여정상대조조대서비교,탕상조대서혈청cTnI함량상후2～48 h균명현증가(P치균소우0.01),기중상후12h체봉치[(5.83 ±0.51) μg/L].탕상조대서심기조직AQP-1 mRNA표체량여심기조직함수솔、혈청cTnI함량균정현저정상관(r치분별위0.849、0.973,P치균소우0.01). 결론 대서탕상후AQP-1가능삼여심기조직수종적형성.
Objective To investigate the changes in aquaporin-1 (AQP-1) expression in myocardium of scalded rats in early stage of a burn injury,and to analyze its relationship with myocardial edema.Methods Thirty-six healthy Wistar rats were divided into normal control (n =6,without scald) and scald (n =30) groups according to the random number table.Rats in scald group were inflicted with 30％ TBSA full-thickness scald on the back,and intraperitoneally injected with Ringer's solution for antishock treatment.Myocardium tissue from left ventricle and serum specimen in rats of scald group were collected at post scald hours (PSH) 2,8,12,24,and 48 (with 6 rats at each time point).Myocardial water ratio was determined by dry-wet weight method.The distribution of AQP-1 protein in myocardium was observed with immunohistochemical staining.The expression of myocardial AQP-1 mRNA was assessed with quantitative realtime PCR.The serum content of cardiac troponin-Ⅰ (cTnⅠ) was determined with ELISA.The rats in normal control group were detected with above-mentioned method.Data were processed with one way analysis of variance and LSD test.Correlation analysis was performed between AQP-1 mRNA and myocardial water ratio,AQP-1 mRNA and the serum content of cTnI in scald group at each time point.Results Compared with that in normal control group,the myocardial water ratio in scald group was markedly increased during PSH 8-48 (P values all below 0.01),and it peaked at PSH 12 [(80.79 ±0.12)％].In both groups,AQP-1 was mainly expressed in endothelial cells of capillaries and pericellular membrane of myocardial cells.The expression of AQP-1 in scald group was markedly incrcased from PSH 2 to PSH 48.The expression of myocardial AQP-1 mRNA in scald group was markedly higher from PSH 2 to PSH 48 than that in normal control group (Pvaluesall below0.01),and it peaked at PSH 12 [(6.2±0.7)％].The serum content ofcTnI in scald group was obviously higher from PSH 2 to PSH 48 than that in normal control group (P values all below 0.01),and it peaked at PSH 12 [(5.83 ± 0.51) μg/L].There were statistically positive correlations between AQP-1 mRNA expression and myocardial water ratio (r =0.849,P ＜0.01),AQP-1 mRNA expression and the serum content of cTnI (r =0.973,P ＜0.01) in scald group.Conclusions AQP-1 may play a key role in the development of myocardial edema in rats with scald.
