目的 分析小鼠严重烧伤后体格发育、学习记忆能力和运动能力的改变,以及体内生长激素(GH)水平的变化趋势. 方法 取104只3~4周龄的健康清洁级BALB/c小鼠,采用随机数字表法分为正常对照组(不作任何处理)、7 s烧伤组(背部造成20% TBSA深Ⅱ度烧伤)、10 s烧伤组(背部造成20% TBSAⅢ度烧伤)、阴性对照组(模拟烧伤组处理过程但不致伤),每组26只.伤前测量各组8只小鼠体质量、体长、尾长.伤后1、3、7d2个烧伤组分别选取6只小鼠采血并处死,采用ELISA试剂盒测定血清中GH水平.将2个烧伤组剩余的8只小鼠饲养至成年,伤后62 d再次测量小鼠的体质量、体长、尾长,计算其增长百分比;伤后66、67、68 d检测小鼠逃避潜伏期,伤后69 d检测小鼠跨越原平台位置次数、目标象限停留时间百分比,以测试小鼠学习记忆能力;伤后70 d检测小鼠负重游泳时间和爬杆时间,测试小鼠运动能力.2个对照组小鼠于相同时相点进行上述检测.对数据行单因素方差分析,两两比较行独立样本t检验;方差不齐则行Welch检验. 结果 (1)7 s烧伤组小鼠伤后1d血清中GH水平为(3 021 ±506) pg/mL,明显低于正常对照组[(3 728±412)pg/mL,t=2.656,P<0.05];伤后3、7d血清中GH水平分别为(3 098±503)、(3 199±275) pg/mL,与正常对照组[(3872±785)、(3 301±600)pg/mL]接近(t值分别为2.033、0.379,P值均大于0.05).10s烧伤组小鼠伤后1、3、7d血清中GH水平分别为(2 859±447)、(2 707±151)、(2 666±222) pg/mL,均明显低于正常对照组(t值分别为3.504、3.571、2.429,P<0.05或P<0.01).(2)7 s烧伤组和10s烧伤组小鼠的体质量增长百分比分别为(112±57)%、(106±36)%,体长增长百分比分别为(32±12)%、(30±8)%,尾长增长百分比分别为(40±15)%、(32±7)%;正常对照组小鼠的体质量、体长、尾长增长百分比分别为(155±22)%、(46±6)%、(63±10)%.除7 s烧伤组体质量增长百分比与正常对照组接近外(t=1.997,P>0.05),2个烧伤组小鼠各项指标增长百分比均明显低于于正常对照组(t值为3.092~6.910,P值均小于0.01).(3)10 s烧伤组小鼠伤后67、68 d逃避潜伏期分别为(33±12)、(33±11)s,明显长于正常对照组[(20±8)、(20±7)s,t值分别为2.421、2.692,P值均小于0.05];伤后69 d跨越原平台位置次数[(1.6±1.8)次]与正常对照组[(3.2±1.5)次]接近(t=1.989,P>0.05);伤后69 d目标象限停留时间百分比[(28±7)%]明显低于正常对照组[(48±16)%,t=3.076,P<0.01].7 s烧伤组小鼠伤后67 d逃避潜伏期为(30±7)s,较正常对照组明显延长(t=2.659,P<0.05);伤后69 d跨越原平台位置次数[(2.0±1.1)次]和目标象限停留时间百分比[(36±1 1)%]与正常对照组接近(t值分别为1.930、1.670,P值均大于0.05).(4)伤后70 d,10 s烧伤组小鼠的爬杆时间、负重游泳时间分别为(30±11)s、(38±39) min,较正常对照组[(47±11)s、(122±74)min]明显缩短(t值分别为3.108、2.868,P<0.05或P<0.01).7 s烧伤组小鼠爬杆时间为(33±14)s,接近正常对照组水平(t=2.044,P>0.05);负重游泳时间为(47±12) min,明显短于正常对照组(t=2.855,P<0.05). 结论 严重烧伤可导致小鼠体格发育不良、学习记忆能力和运动能力下降,同时使体内GH水平明显降低.
目的 分析小鼠嚴重燒傷後體格髮育、學習記憶能力和運動能力的改變,以及體內生長激素(GH)水平的變化趨勢. 方法 取104隻3~4週齡的健康清潔級BALB/c小鼠,採用隨機數字錶法分為正常對照組(不作任何處理)、7 s燒傷組(揹部造成20% TBSA深Ⅱ度燒傷)、10 s燒傷組(揹部造成20% TBSAⅢ度燒傷)、陰性對照組(模擬燒傷組處理過程但不緻傷),每組26隻.傷前測量各組8隻小鼠體質量、體長、尾長.傷後1、3、7d2箇燒傷組分彆選取6隻小鼠採血併處死,採用ELISA試劑盒測定血清中GH水平.將2箇燒傷組剩餘的8隻小鼠飼養至成年,傷後62 d再次測量小鼠的體質量、體長、尾長,計算其增長百分比;傷後66、67、68 d檢測小鼠逃避潛伏期,傷後69 d檢測小鼠跨越原平檯位置次數、目標象限停留時間百分比,以測試小鼠學習記憶能力;傷後70 d檢測小鼠負重遊泳時間和爬桿時間,測試小鼠運動能力.2箇對照組小鼠于相同時相點進行上述檢測.對數據行單因素方差分析,兩兩比較行獨立樣本t檢驗;方差不齊則行Welch檢驗. 結果 (1)7 s燒傷組小鼠傷後1d血清中GH水平為(3 021 ±506) pg/mL,明顯低于正常對照組[(3 728±412)pg/mL,t=2.656,P<0.05];傷後3、7d血清中GH水平分彆為(3 098±503)、(3 199±275) pg/mL,與正常對照組[(3872±785)、(3 301±600)pg/mL]接近(t值分彆為2.033、0.379,P值均大于0.05).10s燒傷組小鼠傷後1、3、7d血清中GH水平分彆為(2 859±447)、(2 707±151)、(2 666±222) pg/mL,均明顯低于正常對照組(t值分彆為3.504、3.571、2.429,P<0.05或P<0.01).(2)7 s燒傷組和10s燒傷組小鼠的體質量增長百分比分彆為(112±57)%、(106±36)%,體長增長百分比分彆為(32±12)%、(30±8)%,尾長增長百分比分彆為(40±15)%、(32±7)%;正常對照組小鼠的體質量、體長、尾長增長百分比分彆為(155±22)%、(46±6)%、(63±10)%.除7 s燒傷組體質量增長百分比與正常對照組接近外(t=1.997,P>0.05),2箇燒傷組小鼠各項指標增長百分比均明顯低于于正常對照組(t值為3.092~6.910,P值均小于0.01).(3)10 s燒傷組小鼠傷後67、68 d逃避潛伏期分彆為(33±12)、(33±11)s,明顯長于正常對照組[(20±8)、(20±7)s,t值分彆為2.421、2.692,P值均小于0.05];傷後69 d跨越原平檯位置次數[(1.6±1.8)次]與正常對照組[(3.2±1.5)次]接近(t=1.989,P>0.05);傷後69 d目標象限停留時間百分比[(28±7)%]明顯低于正常對照組[(48±16)%,t=3.076,P<0.01].7 s燒傷組小鼠傷後67 d逃避潛伏期為(30±7)s,較正常對照組明顯延長(t=2.659,P<0.05);傷後69 d跨越原平檯位置次數[(2.0±1.1)次]和目標象限停留時間百分比[(36±1 1)%]與正常對照組接近(t值分彆為1.930、1.670,P值均大于0.05).(4)傷後70 d,10 s燒傷組小鼠的爬桿時間、負重遊泳時間分彆為(30±11)s、(38±39) min,較正常對照組[(47±11)s、(122±74)min]明顯縮短(t值分彆為3.108、2.868,P<0.05或P<0.01).7 s燒傷組小鼠爬桿時間為(33±14)s,接近正常對照組水平(t=2.044,P>0.05);負重遊泳時間為(47±12) min,明顯短于正常對照組(t=2.855,P<0.05). 結論 嚴重燒傷可導緻小鼠體格髮育不良、學習記憶能力和運動能力下降,同時使體內GH水平明顯降低.
목적 분석소서엄중소상후체격발육、학습기억능력화운동능력적개변,이급체내생장격소(GH)수평적변화추세. 방법 취104지3~4주령적건강청길급BALB/c소서,채용수궤수자표법분위정상대조조(불작임하처리)、7 s소상조(배부조성20% TBSA심Ⅱ도소상)、10 s소상조(배부조성20% TBSAⅢ도소상)、음성대조조(모의소상조처리과정단불치상),매조26지.상전측량각조8지소서체질량、체장、미장.상후1、3、7d2개소상조분별선취6지소서채혈병처사,채용ELISA시제합측정혈청중GH수평.장2개소상조잉여적8지소서사양지성년,상후62 d재차측량소서적체질량、체장、미장,계산기증장백분비;상후66、67、68 d검측소서도피잠복기,상후69 d검측소서과월원평태위치차수、목표상한정류시간백분비,이측시소서학습기억능력;상후70 d검측소서부중유영시간화파간시간,측시소서운동능력.2개대조조소서우상동시상점진행상술검측.대수거행단인소방차분석,량량비교행독립양본t검험;방차불제칙행Welch검험. 결과 (1)7 s소상조소서상후1d혈청중GH수평위(3 021 ±506) pg/mL,명현저우정상대조조[(3 728±412)pg/mL,t=2.656,P<0.05];상후3、7d혈청중GH수평분별위(3 098±503)、(3 199±275) pg/mL,여정상대조조[(3872±785)、(3 301±600)pg/mL]접근(t치분별위2.033、0.379,P치균대우0.05).10s소상조소서상후1、3、7d혈청중GH수평분별위(2 859±447)、(2 707±151)、(2 666±222) pg/mL,균명현저우정상대조조(t치분별위3.504、3.571、2.429,P<0.05혹P<0.01).(2)7 s소상조화10s소상조소서적체질량증장백분비분별위(112±57)%、(106±36)%,체장증장백분비분별위(32±12)%、(30±8)%,미장증장백분비분별위(40±15)%、(32±7)%;정상대조조소서적체질량、체장、미장증장백분비분별위(155±22)%、(46±6)%、(63±10)%.제7 s소상조체질량증장백분비여정상대조조접근외(t=1.997,P>0.05),2개소상조소서각항지표증장백분비균명현저우우정상대조조(t치위3.092~6.910,P치균소우0.01).(3)10 s소상조소서상후67、68 d도피잠복기분별위(33±12)、(33±11)s,명현장우정상대조조[(20±8)、(20±7)s,t치분별위2.421、2.692,P치균소우0.05];상후69 d과월원평태위치차수[(1.6±1.8)차]여정상대조조[(3.2±1.5)차]접근(t=1.989,P>0.05);상후69 d목표상한정류시간백분비[(28±7)%]명현저우정상대조조[(48±16)%,t=3.076,P<0.01].7 s소상조소서상후67 d도피잠복기위(30±7)s,교정상대조조명현연장(t=2.659,P<0.05);상후69 d과월원평태위치차수[(2.0±1.1)차]화목표상한정류시간백분비[(36±1 1)%]여정상대조조접근(t치분별위1.930、1.670,P치균대우0.05).(4)상후70 d,10 s소상조소서적파간시간、부중유영시간분별위(30±11)s、(38±39) min,교정상대조조[(47±11)s、(122±74)min]명현축단(t치분별위3.108、2.868,P<0.05혹P<0.01).7 s소상조소서파간시간위(33±14)s,접근정상대조조수평(t=2.044,P>0.05);부중유영시간위(47±12) min,명현단우정상대조조(t=2.855,P<0.05). 결론 엄중소상가도치소서체격발육불량、학습기억능력화운동능력하강,동시사체내GH수평명현강저.
Objective To analyze the changes in body growth,learning and memory capabilities,exercise capacity,and the level of growth hormone (GH) in mice suffering from severe burn.Methods One hundred and four healthy BALB/c mice aged from 3 to 4 weeks were divided into normal control group (NC,without treatment),7 s burn group (inflicted with 20% TBSA deep partial-thickness burn on the back),10 s burn group (inflicted with 20% TBSA full-thickness burn on the back),and negative control group (simulating the treatment of two burn groups without injury) according to the random number table,with 26 mice in each group.The body weight,body length,and tail length of 8 mice in each group were measured before burn.Respectively 6 mice of two burn groups were sacrificed on post burn day (PBD) 1,3,and 7 for determination of serum level of GH with enzyme-linked immunosorbent assay kit.The remaining 8 mice in each of the two burn groups were raised to adulthood.The body weight,body length,and tail length were measured on PBD 62,and the increased percentages of body weight,body length,and tail length were calculated.Morris water maze tests,including escape latency on PBD 66,67,and 68,frequency of stepping over the platform location and proportion of staying time in the target quadrant on PBD 69,were used to examine the learning and memory capabilities.The time of loaded swimming and the time of pole-climbing on PBD 70 were used to assess the exercise capacity.Above-mentioned indexes were also determined in the two control groups at the same time points.Data were processed with one-way analysis of variance ; Welch test was applied when equal variance was not assumed ; independent samples t test was applied for paired comparison.Results (1) The level of GH of mice in 7 s burn group on PBD 1 [(3 021 ±506) pg/mL] was lower than that of group NC [(3 728±412) pg/mL,t =2.656,P <0.05],while on PBD3 and7 [(3 098 ±503),(3 199 ±275) pg/mL] they were close to those of group NC [(3 872 ±785),(3 301 ± 600) pg/mL,with t values respectively 2.033 and 0.379,P values all above 0.05].The levels of GH of mice in 10 s burn group on PBD 1,3,and 7 were respectively (2 859 ±447),(2 707 ± 151),and (2 666 ± 222) pg/mL,which were lower than those of group NC (with t values respectively 3.504,3.571,2.429,P < 0.05 or P < 0.01).(2) The increased percentage of body weight,body length,and tail length of mice in group NC,7 s burn group,and 10 s burn group were respectively (155 ± 22)%,(46±6)%,(63±10)%; (112±57)%,(32±12)%,(40±15)%; (106±36)%,(30±8) %,(32 ± 7) %.The increased percentage of body weight of mice in 7 s burn group was close to that in group NC (t =1.997,P > 0.05),while the increased percentages of other indexes of mice in the two burn groups were lower than those in group NC (with t values from 3.092 to 6.910,P values all below 0.01).(3) The escape latency values of mice in 10 s burn group on PBD 67 and 68 were respectively (33 ± 12),(33 ± 11) s,which were longer than those of group NC [(20 ± 8),(20 ± 7) s,with t values respectively 2.421 and 2.692,P values all below 0.05].The frequency of stepping over the platform location of mice in 10 s burn group [(1.6 ± 1.8) times] was close to that of group NC [(3.2 ± 1.5) times,t =1.989,P >0.05].The proportion of retention time in the target quadrant of mice in 10 s burn group [(28 ± 7) %] was shorter than that of group NC[(48 ± 16)%,t =3.076,P <0.01].The escape latency of mice in 7 s burn group on PBD 67 [(30 ± 7) s] was longer than that of group NC (t =2.659,P < 0.05).The frequency of stepping over the platform location and the proportion of retention time in the target quadrant of mice in 7 s burn group [(2.0 ± 1.1) times,(36 ± 11) %,respectively] were close to those of group NC (with t values respectively 1.930,1.670,Pvalues all above0.05).(4) The time of pole-climbing [(30 ±11) s] and the time of loaded swimming [(38 ±39) min] of mice in 10 s burn group were shorter than those of group NC [(47±11) s,(122 ±74) min,with t values respectively 3.108,2.868,P <0.05 orP <0.01].The time of loaded swimming of mice in 7 s burn group [(47 ± 12) min] was shorter than that of group NC (t =2.855,P <0.05),while the time of pole-climbing [(33 ±14) s] was close to that of group NC (t =2.044,P >0.05).Conclusions For mice,severe burn can delay body growth,decrease the learning and memory capabilities,decrease exercise capacities,and reduce the serum level of GH.