中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2014年
1期
46-50
,共5页
腐胺%细胞凋亡%肝功能
腐胺%細胞凋亡%肝功能
부알%세포조망%간공능
Putrescine%Apoptosis%Liver function
目的 探讨外源性腐胺对大鼠肝功能和肝细胞凋亡的影响. 方法 将90只健康清洁级SD大鼠按随机数字表法分为3组:对照组10只(腹腔注射生理盐水2mL)、低剂量腐胺组40只、高剂量腐胺组40只.后2组大鼠分别按25、50 μg/g剂量,腹腔注射2.5、5.0 g/L腐胺溶液约2 mL.对照组于注射后24 h,另2组分别于注射后24、48、72、96 h,每组取10只大鼠处死,心脏采血测定血清中ALT、AST含量；摘取肝脏大体观察并行HE染色观察组织形态,原位末端标记法检测肝细胞凋亡情况并计算细胞凋亡指数(AI).对数据行3组间及组内各时相点单因素方差分析或Welch检验,两两比较行LSD或Dunnett T3检验；另行低、高剂量腐胺组两因素析因设计方差分析. 结果 (1)3组大鼠肝脏大体观察无明显异常.对照组大鼠肝组织形态正常；低、高剂量腐胺组大鼠肝组织偶见轻微水肿,未见坏死细胞.(2)低剂量腐胺组大鼠注射后24、48、96 h的ALT含量与注射后72、96 h的AST含量分别为(38±10)、(45±6)、(34±4)、(207±18)、(196±19) U/L,高剂量腐胺组大鼠注射后72、96 h的ALT含量与注射后24、72、96 h的AST含量分别为(38±6)、(48±5)、(213±43)、(209±40)、(230±29) U/L,均分别明显高于对照组注射后24 h的(29±5)、(163±42) U/L(P值均小于0.01).低、高剂量腐胺组大鼠注射后48、72、96 h ALT含量以及注射后96 h AST含量组间比较,差异均有统计学意义(P值均小于0.05).与组内注射后24 h比较,低剂量腐胺组大鼠注射后48 h与高剂量腐胺组大鼠注射后96 h ALT含量显著增高(P值均小于0.05),低剂量腐胺组大鼠注射后48、72、96 h与高剂量腐胺组大鼠注射后48 h AST含量显著增高或降低(P值均小于0.05).低、高剂量腐胺组析因分析显示,不同腐胺浓度对ALT含量的影响差异无统计学意义(F=0.01,P=0.974),但对AST含量的影响存在明显差异(F=12.21,P =0.001).(3)低剂量腐胺组大鼠注射后24、48、72 h的AI值分别为(5.69±0.38)％、(13.80±1.66)％、(11.56±1.74)％,高剂量腐胺组大鼠注射后72、96 h的AI值分别为(10.29±1.43)％、(15.29±1.41)％,均明显高于对照组注射后24 h的(3.50±0.30)％(P值均小于0.01).低、高剂量腐胺组大鼠注射后24、48、96 h AI值组间比较,差异均有统计学意义(P值均小于0.05).与组内注射后24 h比较,低、高剂量腐胺组大鼠注射后48、72、96 h AI值显著增高或降低(P值均小于0.05).低、高剂量腐胺组析因分析显示,不同腐胺浓度及作用时间对AI值的影响均存在明显差异(F值分别为22.95、130.44,P值均小于0.01). 结论 按25、50 μg/g剂量腹腔注射外源性腐胺可导致大鼠一定程度的肝功能损害,诱导肝细胞异常凋亡.腐胺剂量越高,作用时间越长,造成的损害和凋亡程度越明显.
目的 探討外源性腐胺對大鼠肝功能和肝細胞凋亡的影響. 方法 將90隻健康清潔級SD大鼠按隨機數字錶法分為3組:對照組10隻(腹腔註射生理鹽水2mL)、低劑量腐胺組40隻、高劑量腐胺組40隻.後2組大鼠分彆按25、50 μg/g劑量,腹腔註射2.5、5.0 g/L腐胺溶液約2 mL.對照組于註射後24 h,另2組分彆于註射後24、48、72、96 h,每組取10隻大鼠處死,心髒採血測定血清中ALT、AST含量；摘取肝髒大體觀察併行HE染色觀察組織形態,原位末耑標記法檢測肝細胞凋亡情況併計算細胞凋亡指數(AI).對數據行3組間及組內各時相點單因素方差分析或Welch檢驗,兩兩比較行LSD或Dunnett T3檢驗；另行低、高劑量腐胺組兩因素析因設計方差分析. 結果 (1)3組大鼠肝髒大體觀察無明顯異常.對照組大鼠肝組織形態正常；低、高劑量腐胺組大鼠肝組織偶見輕微水腫,未見壞死細胞.(2)低劑量腐胺組大鼠註射後24、48、96 h的ALT含量與註射後72、96 h的AST含量分彆為(38±10)、(45±6)、(34±4)、(207±18)、(196±19) U/L,高劑量腐胺組大鼠註射後72、96 h的ALT含量與註射後24、72、96 h的AST含量分彆為(38±6)、(48±5)、(213±43)、(209±40)、(230±29) U/L,均分彆明顯高于對照組註射後24 h的(29±5)、(163±42) U/L(P值均小于0.01).低、高劑量腐胺組大鼠註射後48、72、96 h ALT含量以及註射後96 h AST含量組間比較,差異均有統計學意義(P值均小于0.05).與組內註射後24 h比較,低劑量腐胺組大鼠註射後48 h與高劑量腐胺組大鼠註射後96 h ALT含量顯著增高(P值均小于0.05),低劑量腐胺組大鼠註射後48、72、96 h與高劑量腐胺組大鼠註射後48 h AST含量顯著增高或降低(P值均小于0.05).低、高劑量腐胺組析因分析顯示,不同腐胺濃度對ALT含量的影響差異無統計學意義(F=0.01,P=0.974),但對AST含量的影響存在明顯差異(F=12.21,P =0.001).(3)低劑量腐胺組大鼠註射後24、48、72 h的AI值分彆為(5.69±0.38)％、(13.80±1.66)％、(11.56±1.74)％,高劑量腐胺組大鼠註射後72、96 h的AI值分彆為(10.29±1.43)％、(15.29±1.41)％,均明顯高于對照組註射後24 h的(3.50±0.30)％(P值均小于0.01).低、高劑量腐胺組大鼠註射後24、48、96 h AI值組間比較,差異均有統計學意義(P值均小于0.05).與組內註射後24 h比較,低、高劑量腐胺組大鼠註射後48、72、96 h AI值顯著增高或降低(P值均小于0.05).低、高劑量腐胺組析因分析顯示,不同腐胺濃度及作用時間對AI值的影響均存在明顯差異(F值分彆為22.95、130.44,P值均小于0.01). 結論 按25、50 μg/g劑量腹腔註射外源性腐胺可導緻大鼠一定程度的肝功能損害,誘導肝細胞異常凋亡.腐胺劑量越高,作用時間越長,造成的損害和凋亡程度越明顯.
목적 탐토외원성부알대대서간공능화간세포조망적영향. 방법 장90지건강청길급SD대서안수궤수자표법분위3조:대조조10지(복강주사생리염수2mL)、저제량부알조40지、고제량부알조40지.후2조대서분별안25、50 μg/g제량,복강주사2.5、5.0 g/L부알용액약2 mL.대조조우주사후24 h,령2조분별우주사후24、48、72、96 h,매조취10지대서처사,심장채혈측정혈청중ALT、AST함량；적취간장대체관찰병행HE염색관찰조직형태,원위말단표기법검측간세포조망정황병계산세포조망지수(AI).대수거행3조간급조내각시상점단인소방차분석혹Welch검험,량량비교행LSD혹Dunnett T3검험；령행저、고제량부알조량인소석인설계방차분석. 결과 (1)3조대서간장대체관찰무명현이상.대조조대서간조직형태정상；저、고제량부알조대서간조직우견경미수종,미견배사세포.(2)저제량부알조대서주사후24、48、96 h적ALT함량여주사후72、96 h적AST함량분별위(38±10)、(45±6)、(34±4)、(207±18)、(196±19) U/L,고제량부알조대서주사후72、96 h적ALT함량여주사후24、72、96 h적AST함량분별위(38±6)、(48±5)、(213±43)、(209±40)、(230±29) U/L,균분별명현고우대조조주사후24 h적(29±5)、(163±42) U/L(P치균소우0.01).저、고제량부알조대서주사후48、72、96 h ALT함량이급주사후96 h AST함량조간비교,차이균유통계학의의(P치균소우0.05).여조내주사후24 h비교,저제량부알조대서주사후48 h여고제량부알조대서주사후96 h ALT함량현저증고(P치균소우0.05),저제량부알조대서주사후48、72、96 h여고제량부알조대서주사후48 h AST함량현저증고혹강저(P치균소우0.05).저、고제량부알조석인분석현시,불동부알농도대ALT함량적영향차이무통계학의의(F=0.01,P=0.974),단대AST함량적영향존재명현차이(F=12.21,P =0.001).(3)저제량부알조대서주사후24、48、72 h적AI치분별위(5.69±0.38)％、(13.80±1.66)％、(11.56±1.74)％,고제량부알조대서주사후72、96 h적AI치분별위(10.29±1.43)％、(15.29±1.41)％,균명현고우대조조주사후24 h적(3.50±0.30)％(P치균소우0.01).저、고제량부알조대서주사후24、48、96 h AI치조간비교,차이균유통계학의의(P치균소우0.05).여조내주사후24 h비교,저、고제량부알조대서주사후48、72、96 h AI치현저증고혹강저(P치균소우0.05).저、고제량부알조석인분석현시,불동부알농도급작용시간대AI치적영향균존재명현차이(F치분별위22.95、130.44,P치균소우0.01). 결론 안25、50 μg/g제량복강주사외원성부알가도치대서일정정도적간공능손해,유도간세포이상조망.부알제량월고,작용시간월장,조성적손해화조망정도월명현.
Objective To explore the influence of exogenous putrescine on the function of liver and apoptosis of liver cells in rats.Methods Ninety healthy clean SD rats were divided into control group (C,n =10,intraperitoneally injected with 2 mL normal saline),low dosage putrescine group (LP,n =40),and high dosage putrescine group (HP,n =40) according to the random number table.Rats in the latter two groups were intraperitoneally injected with approximately 2 mL putrescine (2.5 or 5.0 g/L) with the dosage of 25 or 50 μg/g.Ten rats from group C at post injection hour (PIH) 24 and 10 rats from each of the latter two groups at PIH 24,48,72,96 were sacrificed.Heart blood was obtained for determination of serum contents of ALT and AST.Liver was harvested for gross observation and histomorphological observation with HE staining.Apoptosis was shown with in situ end labeling,and apoptosis index (AI) was calculated.Data among the three groups and those at different time points within one group were processed with one-way analysis of variance or Welch test; LSD or Dunnett's T3 test was used for paired comparison; factorial design analysis of variance of two factors was applied for data between group LP and group HP.Results (1) No obvious abnormality was observed at gross observation of liver of rats in each group.Liver tissue of rats in group C was normal.Light edema was observed occasionally in liver of rats in groups LP and HP,but necrotic cells were not seen.(2) Content of ALT at PIH 24,48,96 and content of AST at PIH 72 and 96in group LP were respectively (38 ± 10),(45 ±6),(34 ±4),(207 ± 18),(196 ± 19) U/L,and content of ALT at PIH 72 and 96 and content of AST at PIH 24,72,96 in group HP were respectively (38 ±6),(48 ±5),(213 ±43),(209 ±40),(230 ±29) U/L.They were significantly higher than those of rats in group C [(29 ± 5),(163 ± 42) U/L,with P values all below 0.01].There were statistically significant differences between group LP and group HP in the content of ALT at PIH 48,72,96 and content of AST at PIH 96 (with P values all below 0.05).Compared with that at PIH 24 of each group,content of ALT of rats in group LP at PIH 48 and that of rats in group HP at PIH 96,as well as content of AST of rats in group LP at PIH 48,72,96 and that of rats in group HP at PIH 48 were significantly increased or decreased (with P values all below 0.05).Factorial analysis showed that the differences due to different concentration of putrescine on content of AST were statistically significant (F =12.21,P =0.001),but not on content of ALT (F =0.01,P =0.974) between group LP and group HP.(3) AI values of rats in group LPat PIH24,48,72 were respectively (5.69 ±0.38)％,(13.80 ± 1.66)％,(11.56 ± 1.74)％,and AI values of rats in group HP at PIH 72 and 96 were respectively (10.29 ± 1.43) ％,(15.29 ± 1.41) ％.They were all obviously higher than AI value of control group at PIH 24 [(3.50 ± 0.30) ％,with P values all below 0.01].There were statistically significant differences between group LP and group HP in AI value at PIH 24,48,96(with P values all below 0.05).Compared with that at PIH 24 of each group,AI value of rats in groups LP and HP at PIH 48,72,96 were significantly increased or decreased (with P values all below 0.05).Factorial analysis showed that the differences in the influence of concentration of putrescine and stimulation time on AI value were statistically significant (with F values respectively 22.95 and 130.44,P values all below 0.01).Conclusions Intraperitoneal injection of exogenous putrescine in the dosage of 25 or 50 μg/g could lead to certain degree of functional damage of liver and apoptosis of liver cells of rat.The higher the dosage and the longer the stimulation time,the more obvious the damage and apoptosis would be.
