中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2013年
2期
112-114
,共3页
郝红晓%张艳丽%李明慧%张禄雪%易为%胡玉红%伊诺%成军%刘顺爱
郝紅曉%張豔麗%李明慧%張祿雪%易為%鬍玉紅%伊諾%成軍%劉順愛
학홍효%장염려%리명혜%장록설%역위%호옥홍%이낙%성군%류순애
树突细胞%分子诊断技术%肝炎病毒,乙型%婴儿,新生
樹突細胞%分子診斷技術%肝炎病毒,乙型%嬰兒,新生
수돌세포%분자진단기술%간염병독,을형%영인,신생
Dendritic cells%Molecular diagnostic techniques%Hepatitis B virus%Infant,newborn
目的 探讨新生儿mDC、pDC频数及表面分子表达,以及母亲不同HBV感染状态对新生儿树突状细胞生物学特性的影响.方法 采集HBsAg阳性/HBeAg阳性HBV感染母亲、HBsAg阳性/HBeAg阴性HBV感染母亲以及HBV感染标志物阴性母亲所生新生儿脐带血、健康成人外周血,采用流式细胞仪检测mDC的频数及其CD86的表达、pDC的频数及其CD80、CD83、CD86表达、并采用FlowJo软件进行分析,比较各组间上述指标的差异.结果 分别采集HBsAg阳性/HBeAg阳性、HBsAg阳性/HBeAg阴性和HBV感染标志物阴性母亲所生新生儿脐带血14、12和13例,健康成人外周血7例.新生儿mDC频数(0.29±0.16)及其CD86阳性率(10.72±10.01)显著低于成年人(分别是0.81±0.17和32.13±7.46),(t=-7.86,P=0.00和t=-5.36,P=0.00)；新生儿pDC频数(0.15±0.07)以及pDC表面CD86/CD83阳性率(31.61±12.81,42.66±20.83)显著低于成年人(0.30±0.07;74.96±9.78;82.00±6.94),(t=-5.43,P =0.00;t=-8.49,P=0.00;t=-4.90,P=0.00).结论 新生儿脐带血mDC、pDC频数以及表面功能分子表达低于成人外周血,HBeAg可能降低生新生儿mDC表面CD86的表达.
目的 探討新生兒mDC、pDC頻數及錶麵分子錶達,以及母親不同HBV感染狀態對新生兒樹突狀細胞生物學特性的影響.方法 採集HBsAg暘性/HBeAg暘性HBV感染母親、HBsAg暘性/HBeAg陰性HBV感染母親以及HBV感染標誌物陰性母親所生新生兒臍帶血、健康成人外週血,採用流式細胞儀檢測mDC的頻數及其CD86的錶達、pDC的頻數及其CD80、CD83、CD86錶達、併採用FlowJo軟件進行分析,比較各組間上述指標的差異.結果 分彆採集HBsAg暘性/HBeAg暘性、HBsAg暘性/HBeAg陰性和HBV感染標誌物陰性母親所生新生兒臍帶血14、12和13例,健康成人外週血7例.新生兒mDC頻數(0.29±0.16)及其CD86暘性率(10.72±10.01)顯著低于成年人(分彆是0.81±0.17和32.13±7.46),(t=-7.86,P=0.00和t=-5.36,P=0.00)；新生兒pDC頻數(0.15±0.07)以及pDC錶麵CD86/CD83暘性率(31.61±12.81,42.66±20.83)顯著低于成年人(0.30±0.07;74.96±9.78;82.00±6.94),(t=-5.43,P =0.00;t=-8.49,P=0.00;t=-4.90,P=0.00).結論 新生兒臍帶血mDC、pDC頻數以及錶麵功能分子錶達低于成人外週血,HBeAg可能降低生新生兒mDC錶麵CD86的錶達.
목적 탐토신생인mDC、pDC빈수급표면분자표체,이급모친불동HBV감염상태대신생인수돌상세포생물학특성적영향.방법 채집HBsAg양성/HBeAg양성HBV감염모친、HBsAg양성/HBeAg음성HBV감염모친이급HBV감염표지물음성모친소생신생인제대혈、건강성인외주혈,채용류식세포의검측mDC적빈수급기CD86적표체、pDC적빈수급기CD80、CD83、CD86표체、병채용FlowJo연건진행분석,비교각조간상술지표적차이.결과 분별채집HBsAg양성/HBeAg양성、HBsAg양성/HBeAg음성화HBV감염표지물음성모친소생신생인제대혈14、12화13례,건강성인외주혈7례.신생인mDC빈수(0.29±0.16)급기CD86양성솔(10.72±10.01)현저저우성년인(분별시0.81±0.17화32.13±7.46),(t=-7.86,P=0.00화t=-5.36,P=0.00)；신생인pDC빈수(0.15±0.07)이급pDC표면CD86/CD83양성솔(31.61±12.81,42.66±20.83)현저저우성년인(0.30±0.07;74.96±9.78;82.00±6.94),(t=-5.43,P =0.00;t=-8.49,P=0.00;t=-4.90,P=0.00).결론 신생인제대혈mDC、pDC빈수이급표면공능분자표체저우성인외주혈,HBeAg가능강저생신생인mDC표면CD86적표체.
Objective The aim of this study was to explore the frequency of mDC and pDC and expression of surface markers of the neonates and to discuss the effect of different status of HBV infection of mother on biological characteristics of DC.Methods Umbilicus cord blood in neonates of HBeAg positive HBV infected mother,HBeAg negative HBV infected mother,and normal mother were collected respectively ; peripheral blood of healthy adults were selected as control group.Flow cytometry was employed to detect frequency of the mDC and its expression of CD86,frequency of pDC and its expression of CD80,CD83,CD86,and FlowJo software was used to compare these indicators among the groups.Results Compared with control group,the frequency of mDC of cord blood(0.29 ± 0.16 vs 0.81 ± 0.17),CD86 positive rate ofmDC (10.72 ±10.01 vs 32.13 ±7.46),the frequency of pDC (0.15 ±0.07 vs 0.30 ±0.07),and CD86/CD83 positive rate of pDC (31.61 ± 12.81 vs 74.96 ± 9.78 ;42.66 ± 20.83 vs 82.00 ±6.94)were lower (t =-7.86,P =0.00;t =-5.36,P =0.00;t =-5.43,P =0.00;t =-8.49.P =0.00;t =-4.90,P =0.00).Conclusions The frequency of mDC and pDC in umbilical cord blood was lower than the peripheral blood of healthy adult,which was the possible mechanism of newborns easier to chronicity after the infection of hepatitis B virus.A significant correlation was found between different status of HBV infection and costimulatory molecule CD86 positive rate of mDC,but not for the frequency of mDC and pDC,and the expression of pDC molecules.
