CAJ | 학술논문

目的建立人细小病毒B19、人博卡病毒(HBoV)及人细小病毒4(PARV4)分子检测方法,并应用于南京95例肝病患者血浆中三种人细小病毒的感染情况分析.方法分别建立人细小病毒B19、HBoV及PARV4感染的巢式PCR方法,确定其灵敏度与特异性；并对南京市某医院95例住院肝病患者血浆中三种人细小病毒的感染情况进行检测,并对检测结果进行统计学分析.结果建立的三种巢式PCR方法特异性好,检测灵敏度均可达10个拷贝.95例肝病患者血浆中检出B19感染2例(2.1％),HBoV感染9例(9.5％),且发现在5例药物性肝炎患者中检出HBoV阳性3例,但未检出PARV4.结论人细小病毒B19、人博卡病毒(HBoV)及人细小病毒4(PARV4)巢式PCR检测方法灵敏特异.从肝病患者血浆中检出了B19及HBoV.
목적 건립인세소병독B19、인박잡병독(HBoV)급인세소병독4(PARV4)분자검측방법,병응용우남경95례간병환자혈장중삼충인세소병독적감염정황분석.방법 분별건립인세소병독B19、HBoV급PARV4감염적소식PCR방법,학정기령민도여특이성；병대남경시모의원95례주원간병환자혈장중삼충인세소병독적감염정황진행검측,병대검측결과진행통계학분석.결과 건립적삼충소식PCR방법특이성호,검측령민도균가체10개고패.95례간병환자혈장중검출B19감염2례(2.1％),HBoV감염9례(9.5％),차발현재5례약물성간염환자중검출HBoV양성3례,단미검출PARV4.결론 인세소병독B19、인박잡병독(HBoV)급인세소병독4(PARV4)소식PCR검측방법령민특이.종간병환자혈장중검출료B19급HBoV.
Objective To analyze the infection of human parvovirus B19,human bocavirus(HBoV) and human parvovirus 4 (PARV4) in blood samples among patients with liver disease in Nanjing by molecular detection.Methods Nested PCR assays were designed and validated to detect B19,HBoV and PARV4,respectively.The assays were used to screen three parvoviruses in blood samples from 95 patients with different liver disease in Nanjing.The parvovirus infection was analyzed statistically.Results The detection limits were 10 copies of genomic DNA equivalents per reaction for each assays and the good specificity were observed.The frequency of B19 and HBoV were 2/95 (2.1％)and 9/95 (9.5％)in blood samples respectively.No PARV4 was detected.HBoV was detected in 3/5 patients with drug-induced hepatitis.Conclusion Both B19 and HBoV infection were detected in blood from patients with liver disease.