CAJ | 학술논문

目的探讨CXCL12/CXCR4生物轴对胰腺癌细胞增殖、侵袭等生物学行为的影响.方法体外培养胰腺癌细胞系Miapaca-2,将其分为对照组、CXCL12组和AMD3100组.(1)采用RT-PCR检测胰腺癌细胞中CXCL12、CXCR4、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)和人尿激酶型纤溶酶原激活物(uPA) mRNA的表达水平;(2)采用CCK-8法检测各组细胞的增殖情况;(3)采用Transwell侵袭实验检测CXCL12/CXCR4对胰腺癌细胞趋化活性的影响.结果胰腺癌细胞系Miapaca-2中CXCL12 mRNA未见表达,而CXCR4 mRNA在胰腺癌细胞中有表达.MMP-2、MMP-9和uPA mRNA在AMD3100组、对照组和CXCL12组中的表达水平呈递增趋势,差异具有统计学意义(P＜0.05).胰腺癌细胞的增殖和侵袭能力在CXCL12组明显增强,而在AMD3100组得到了有效的抑制,组间差异有统计学意义(P＜0.05).结论趋化因子CXCL12及其受体CXCR4所构成的生物轴对胰腺癌细胞的增殖和侵袭能力发挥着重要的作用.
목적 탐토CXCL12/CXCR4생물축대이선암세포증식、침습등생물학행위적영향.방법 체외배양이선암세포계Miapaca-2,장기분위대조조、CXCL12조화AMD3100조.(1)채용RT-PCR검측이선암세포중CXCL12、CXCR4、기질금속단백매-2(MMP-2)、기질금속단백매-9(MMP-9)화인뇨격매형섬용매원격활물(uPA) mRNA적표체수평;(2)채용CCK-8법검측각조세포적증식정황;(3)채용Transwell침습실험검측CXCL12/CXCR4대이선암세포추화활성적영향.결과 이선암세포계Miapaca-2중CXCL12 mRNA미견표체,이CXCR4 mRNA재이선암세포중유표체.MMP-2、MMP-9화uPA mRNA재AMD3100조、대조조화CXCL12조중적표체수평정체증추세,차이구유통계학의의(P＜0.05).이선암세포적증식화침습능력재CXCL12조명현증강,이재AMD3100조득도료유효적억제,조간차이유통계학의의(P＜0.05).결론 추화인자CXCL12급기수체CXCR4소구성적생물축대이선암세포적증식화침습능력발휘착중요적작용.
Objective The aim of this study is to explore the effect of CXCL12/CXCR4 biological axis on biological behavior of pancreatic cancer cells through the detection of mRNA expressions.Methods Pancreatic cancer cells (Miapaca-2) were cultured in vitro and divided into the control group,CXCL12 group and AMD3100 group.(1)RT-PCR was applied to detect the mRNA expressions of CXCL12,CXCR4,matrix metalloproteinase 2 (MMP-2),MMP-9 and human urokinase plasminogen activator (uPA) ; (2) CCK-8 methods was used to detect the proliferation of cells; (3) Transwell invasion assay was applied to detect the invasive ability of pancreatic cancer cells among groups respectively.Results The mRNA expression of CXCL12 was not found,while the mRNA expression of CXCR4 was observed in pancreatic cancer cells.There was a tendency of decreasing on the mRNA expressions of MMP-2,MMP-9 and uPA among three groups,and there was statistically significant difference (P ＜ 0.05).The proliferative and invasive ability of pancreatic cancer cells showed an enhanced trend in CXCL12 group,while in AMD3100 group was effectively inhibited.There was statistically significant difference among three groups (P ＜ 0.05).Conclusions CXCL12/CXCR4 biological axis plays an important role in proliferation and invasion of pancreatic cancer cells.