CAJ | 학술논문

目的比较DC-LMP2与rAd-LMP2在BALB/c小鼠中的免疫效果.方法从BALB/c小鼠中分离骨髓细胞,在细胞因子作用下体外诱导培养获得小鼠树突状细胞(mDC),再以100 MOI的含EB病潜伏膜蛋白2的重组腺病毒(rAd-LMP2)感染,获得DC-LMP2,免疫酶法确定LMP2在DC中表达.于0,2,4周,分别以PBS、DC-LMP2和rAd-LMP2肌肉免疫BALB/c小鼠,第5和8周检测小鼠体内LMP2特异性细胞应答水平.结果骨髓细胞体外成功诱导获得DC,LMP2在DC中表达.DC-LMP2和rAd-LMP2均可诱导LMP2特异性细胞免疫应答,随时间推移而减弱.rAd-LMP2诱导特异性细胞免疫应答水平高于DC-LMP2.结论肌肉免疫rAd-LMP2能够在BALB/c小鼠中诱导较强的LMP2特异性细胞免疫应答.
목적 비교DC-LMP2여rAd-LMP2재BALB/c소서중적면역효과.방법 종BALB/c소서중분리골수세포,재세포인자작용하체외유도배양획득소서수돌상세포(mDC),재이100 MOI적함EB병잠복막단백2적중조선병독(rAd-LMP2)감염,획득DC-LMP2,면역매법학정LMP2재DC중표체.우0,2,4주,분별이PBS、DC-LMP2화rAd-LMP2기육면역BALB/c소서,제5화8주검측소서체내LMP2특이성세포응답수평.결과 골수세포체외성공유도획득DC,LMP2재DC중표체.DC-LMP2화rAd-LMP2균가유도LMP2특이성세포면역응답,수시간추이이감약.rAd-LMP2유도특이성세포면역응답수평고우DC-LMP2.결론 기육면역rAd-LMP2능구재BALB/c소서중유도교강적LMP2특이성세포면역응답.
Objective To compare immunological effect induced with DC-LMP2 and rAd-LMP2 in BALB/c mice.Mehtods Bone marrow cells were separated from BALB/c mice,and induced into murine dendritic cells (mDCs) cultured by RPMI 1640 medium supplemented with 10％ fetal calf serum and recombinant murine cytokines in vitro.mDCs were infected by recombinant adenovirus including latent membrane protein Ⅱ (LMP2) gene (rAd-LMP'2) of Epstein-Barr virus(EBV) at a dose of 100 multiplicity of infection (MOI),and matured mDC-LMP2 was harvested at 48 hours later.The LMP2 expression in mDCs was detected by immunoenzyme.BALB/c mice were immunized with PBS DC-LMP2 and rAd-LMP2 by intramuscular injection at 0,2,4 weeks.LMP2 specific cytotoxic lymphocytes (CTLs) in BALB/c mice were detected at 5th week and 8th week with enzyme-linked immunosorbent spot (ELISPOT).Results mDCs from bone marrow cells were harvested.LMP2 was detected in DC-LMP2.LMP2 specific CTLs were stimulated by both DC-LMP2 and rAd-LMP2 and decreased with time elapsed.LMP2 specific CTLs stimulated by rAd-LMP2 were higher than that by DC-LMP2 in BALB/c mice.Conclusion Immunization with rAd-LMP2 by intramuscular injection was more fitful than that with DC-LMP2 in BALB/c mice.