CAJ | 학술논문

目的探讨血清学检测方法和实时荧光定量PCR方法在新型布尼亚病毒检测中的应用.方法连续采集烟台地区莱州、蓬莱疑似发热伴血小板减少综合征患者血清,分别用胶体金实验方法、ELISA实验方法检测抗新型布尼亚病毒IgM抗体,实时荧光定量PCR方法检测新型布尼亚病毒核酸,SPSS18.0统计结果.结果胶体金方法和ELISA方法检测结果总符合率为96.67％,一致性KAPPA检验κ系数为0.933;胶体金方法和实时荧光定量PCR方法检测结果均受病程影响(两者P值均小于0.05);实时荧光定量PCR方法检测不受年龄、性别影响(P均＞0.05).结论新型布尼亚病毒检测发病12天以前推荐使用实时荧光定量PCR方法,发病16天以后,可使用血清学方法检测抗新型布尼亚病毒IgM抗体.
목적 탐토혈청학검측방법화실시형광정량PCR방법재신형포니아병독검측중적응용.방법 련속채집연태지구래주、봉래의사발열반혈소판감소종합정환자혈청,분별용효체금실험방법、ELISA실험방법검측항신형포니아병독IgM항체,실시형광정량PCR방법검측신형포니아병독핵산,SPSS18.0통계결과.결과 효체금방법화ELISA방법검측결과총부합솔위96.67％,일치성KAPPA검험κ계수위0.933;효체금방법화실시형광정량PCR방법검측결과균수병정영향(량자P치균소우0.05);실시형광정량PCR방법검측불수년령、성별영향(P균＞0.05).결론 신형포니아병독검측발병12천이전추천사용실시형광정량PCR방법,발병16천이후,가사용혈청학방법검측항신형포니아병독IgM항체.
Objective To compare serological methods with real time RT-PCR in detection of severe fever with thrombocytopenia syndrome Bunyavirus.Method Serum of severe fever with thrombocytopenia syndrome was collected in Penglai and Laizhou in Yantai.Anti-severe fever with thrombocytopenia syndrome Bunyavirus (SFTSV) IgM was detected using gold colloid and ELISA,nucleic acid of severe fever with thrombocytopenia syndrome Bunyavirus was detected by real-time PCR.Statistics of the results were done using SPSS 18.0.Result The coincidence of ELISA and gold colloid was 96.97％ and the kcoefficient was 0.933 ;the course of disease influences the results notablely both gold colloid and real-time PCR(P ＜ 0.05) and real-time PCR was not influenced by patients age or sex(P ＞ 0.05).Conclusion When the course of disease less than 12 days,real-time PCR was recommended and when the course of disease more than 16 days,serological methods can use to detect the anti-SFTSV IgM.