CAJ | 학술논문

目的建立扩增受阻突变检测系统(ARMS)检测乙型肝炎病毒(HBV)基因型方法,并对慢性乙型肝炎(CHB)患者HBV基因型进行分析.方法通过对HBV全基因组序列比对分析,设计ARMS特异性引物和探针体系,建立检测HBV B和C基因型方法,对50例临床标本进行检测.结果 50例临床样本中,B型的检出率为26％(1 3例),C型为74％(37例),其实验结果与测序结果完全一致.常州及周边地区乙型肝炎病毒C基因型HBV为优势株.C基因型患者A1762T/G1764A突变率为62％,明显高于B型.结论 ARMS检测HBV基因型,快速、准确;常州及周边地区HBV基因型主要为B、C型,且C型携带A1762T/G1764A双突变率较高,与较为严重的肝病相关.
목적 건립확증수조돌변검측계통(ARMS)검측을형간염병독(HBV)기인형방법,병대만성을형간염(CHB)환자HBV기인형진행분석.방법 통과대HBV전기인조서렬비대분석,설계ARMS특이성인물화탐침체계,건립검측HBV B화C기인형방법,대50례림상표본진행검측.결과 50례림상양본중,B형적검출솔위26％(1 3례),C형위74％(37례),기실험결과여측서결과완전일치.상주급주변지구을형간염병독C기인형HBV위우세주.C기인형환자A1762T/G1764A돌변솔위62％,명현고우B형.결론 ARMS검측HBV기인형,쾌속、준학;상주급주변지구HBV기인형주요위B、C형,차C형휴대A1762T/G1764A쌍돌변솔교고,여교위엄중적간병상관.
Objective To Establish amplification refractory mutation system (ARMS) for hepatitis B virus genotyping assay and detect HBV genotype in patients with chronic hepatitis B by it.Method Based on HBV genome sequence alignment,we designed specific primers and probe,established ARMS for HBV B and C genotyping and detected 50 clinical samples with chronic HBV infection.Results Among 50 samples,genotype B and C which were detected by ARMS assay is 26％ (14/50) and 74％ (37/50) separately.The results were completely consistent with sequencing results.Genotype C was more prevalent than genotype B in Changzhou area,and 62％ patients with genotype C carried A1762T/G1764A double mutation,much higher than genotype B.Conclusion ARMS was a fast and accurate assay for HBV genotype detection.Dominant HBV genotypes in Changzhou area were genotypes B and C,and genotype C carried more A1762T/G1764A double mutation,maybe associated with more sever liver disease.