中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
1期
4-6
,共3页
贾凌%王洁%施军平%王福根%方红英%朱梦飞%娄国强
賈凌%王潔%施軍平%王福根%方紅英%硃夢飛%婁國彊
가릉%왕길%시군평%왕복근%방홍영%주몽비%루국강
乙酰半胱氨酸%炭%脂肪肝%抗氧化剂
乙酰半胱氨痠%炭%脂肪肝%抗氧化劑
을선반광안산%탄%지방간%항양화제
Acetylcysteine%Charcoal%Fatty liver%Antioxidants
目的 探讨乙酰半胱氨酸纳米活性碳缓释微囊(ACNAC)对非酒精性脂肪性肝炎(NASH)大鼠肝脏抗氧化能力的影响.方法 通过高脂饮食12周诱导建立NASH大鼠动物模型,分别给予ACNAC高、中、低每日不同剂量(800 mg/kg、400 mg/kg和200 mg/kg)与易善复(0.692 mg/kg)、乙酰半胱氨酸(400 mg/kg)连续灌胃8周后,检测血清生化指标、肝匀浆丙二醛(MDA)含量、总超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)活性及肝脏病理指标.结果 ACNAC中剂量组与高脂组相比,血清ALT、AST明显下降(P＜0.01 、P ＜0.05),且优于易善复组(P＜0.05).血清TCHOL 、TG、LDL-C和CR检测结果差异虽无统计学意义,但其数值上ACNAC明显低于高脂组,且优于易善复组和NAC组.ACNAC高、中、低三组与高脂组相比,SOD活力明显升高(P ＜0.01) 、GSH含量明显升高(P＜0.01),ACNAC中剂量组与高脂组比较GSH-PX活性明显升高(P＜0.05)但略低于易善复组(P＜0.01)).结论 相关剂量的乙酰半胱氨酸纳米活性炭缓释微囊可增强非酒精性脂肪性肝炎大鼠的抗氧化能力.
目的 探討乙酰半胱氨痠納米活性碳緩釋微囊(ACNAC)對非酒精性脂肪性肝炎(NASH)大鼠肝髒抗氧化能力的影響.方法 通過高脂飲食12週誘導建立NASH大鼠動物模型,分彆給予ACNAC高、中、低每日不同劑量(800 mg/kg、400 mg/kg和200 mg/kg)與易善複(0.692 mg/kg)、乙酰半胱氨痠(400 mg/kg)連續灌胃8週後,檢測血清生化指標、肝勻漿丙二醛(MDA)含量、總超氧化物歧化酶(SOD)活性、還原型穀胱甘肽(GSH)、穀胱甘肽過氧化物酶(GSH-Px)活性及肝髒病理指標.結果 ACNAC中劑量組與高脂組相比,血清ALT、AST明顯下降(P＜0.01 、P ＜0.05),且優于易善複組(P＜0.05).血清TCHOL 、TG、LDL-C和CR檢測結果差異雖無統計學意義,但其數值上ACNAC明顯低于高脂組,且優于易善複組和NAC組.ACNAC高、中、低三組與高脂組相比,SOD活力明顯升高(P ＜0.01) 、GSH含量明顯升高(P＜0.01),ACNAC中劑量組與高脂組比較GSH-PX活性明顯升高(P＜0.05)但略低于易善複組(P＜0.01)).結論 相關劑量的乙酰半胱氨痠納米活性炭緩釋微囊可增彊非酒精性脂肪性肝炎大鼠的抗氧化能力.
목적 탐토을선반광안산납미활성탄완석미낭(ACNAC)대비주정성지방성간염(NASH)대서간장항양화능력적영향.방법 통과고지음식12주유도건립NASH대서동물모형,분별급여ACNAC고、중、저매일불동제량(800 mg/kg、400 mg/kg화200 mg/kg)여역선복(0.692 mg/kg)、을선반광안산(400 mg/kg)련속관위8주후,검측혈청생화지표、간균장병이철(MDA)함량、총초양화물기화매(SOD)활성、환원형곡광감태(GSH)、곡광감태과양화물매(GSH-Px)활성급간장병리지표.결과 ACNAC중제량조여고지조상비,혈청ALT、AST명현하강(P＜0.01 、P ＜0.05),차우우역선복조(P＜0.05).혈청TCHOL 、TG、LDL-C화CR검측결과차이수무통계학의의,단기수치상ACNAC명현저우고지조,차우우역선복조화NAC조.ACNAC고、중、저삼조여고지조상비,SOD활력명현승고(P ＜0.01) 、GSH함량명현승고(P＜0.01),ACNAC중제량조여고지조비교GSH-PX활성명현승고(P＜0.05)단략저우역선복조(P＜0.01)).결론 상관제량적을선반광안산납미활성탄완석미낭가증강비주정성지방성간염대서적항양화능력.
Objective To explore the effect of N-acetylcysteine nano-carbon (ACNAC) on antioxidative capacity in non-alcoholic steatohepatitis rats.Methods Rats non-alcoholic steatohepatitis model was established by means of high-fat diet in 12 weeks.Then the rats were treated with different doses of ACNAC (800 mg/kg,400 mg/kg and 200 mg/kg) and YISHANFU at the dose of 0.692 mg/kg,NAC at the dose of 400 mg/kg in astrolavaging perday.After 8 weeks,serological levels and liver tissue of SOD,MDA,GSH,GSH-PX in all groups were measured.Pathological changes of liver tissues under light microscope were observed.Results Compared with the high-fat group rats,the groups of medium dose and low dose of ACNAC in the serological levels of ALT,AST were decreased significantly,even lower than the group of YISHANFU.TCHOL,TG,LDL-C and CR were no statistical significance but decresed in date, and also lower than the groups of YISHANFU and NAC.The level of liver tissue in SOD,GSH,GSH-PX,the group of ACNAC were higher than the high-fat group's,and the level of MDA was lower than that.Conclusion Dose-related N-acetylcysteine Nano-carbon could enhance antioxidant capacity of NASH rats.
