CAJ | 학술논문

目的了解发热伴血小板减少综合征布尼亚病毒(SFTSV)细胞嗜性,加强对SFTSV传播与致病机制的理解.方法通过不同感染复数(multiplicity of infection)的SFTSV感染Vero等10种不同组织来源的细胞株,采用双抗体夹心ELISA法和间接免疫荧光实验(IFA)检测不同时间点的细胞培养上清和细胞内病毒抗原表达水平,绘制病毒动态生长曲线,并观察记录细胞病变(cytopathic effect,CPE)情况,确定SFTSV细胞嗜性.结果 Vero、HEK 293、COS-7、CHO、HepG2、Hela、THP-1和MRC-5等不同组织来源的细胞均可感染SFTSV,并产生明显细胞病变,但病毒在不同细胞中的复制水平差异显著,其中Vero细胞对SFTSV较易感,病毒可增殖至10S0TCID50/ml以上；Raji细胞、Jurkat细胞不能感染SFTSV.结论 SFTSV具有广嗜性,可以感染肝、肺、肾、子宫和卵巢等多器官以及免疫系统等来源的细胞系,但不能感染T和B淋巴细胞源细胞系,为SFTSV相关基础研究提供重要线索.
목적 료해발열반혈소판감소종합정포니아병독(SFTSV)세포기성,가강대SFTSV전파여치병궤제적리해.방법 통과불동감염복수(multiplicity of infection)적SFTSV감염Vero등10충불동조직래원적세포주,채용쌍항체협심ELISA법화간접면역형광실험(IFA)검측불동시간점적세포배양상청화세포내병독항원표체수평,회제병독동태생장곡선,병관찰기록세포병변(cytopathic effect,CPE)정황,학정SFTSV세포기성.결과 Vero、HEK 293、COS-7、CHO、HepG2、Hela、THP-1화MRC-5등불동조직래원적세포균가감염SFTSV,병산생명현세포병변,단병독재불동세포중적복제수평차이현저,기중Vero세포대SFTSV교역감,병독가증식지10S0TCID50/ml이상；Raji세포、Jurkat세포불능감염SFTSV.결론 SFTSV구유엄기성,가이감염간、폐、신、자궁화란소등다기관이급면역계통등래원적세포계,단불능감염T화B림파세포원세포계,위SFTSV상관기출연구제공중요선색.
Objective To learn the susceptibility of culture cells to Severe fever with thrombocytopenia syndrome virus (SFTSV) and strengthen the understanding for transmission and pathogenic mechanism of SFTSV.Method SFTSV infected different cells which derived from 10 cell lines such asVero cells by different multiplicity of infection (MOI).The titration of cell culture supernatant at different time points and the expression of virus antigen were measured by double antibody sandwich ELISA and indirect immunofluorescence assay (IFA).By drawing dynamic growth curve of SFTSV and observing cytopathic effect (CPE) to determine the cell tropism of SFTSV.Result The results suggested that SFTSV can affect Vero,HEK 293,COS-7,CHO,HepG2,Hela,THP-1 and MRC-5,and significant CPE can be observed,but the virus replication levels are significantly different in these cells.Vero cells can relatively susceptible to SFTSV,and the virus titration can reach 1080 TCID50/ml.SFTSV cannot susceptibly affect Raji cells and Jurkat cells.Conclusion SFTSV has broad tissue and cell tropism,and it can infect liver,lung,kidney,uterus,ovaries and other organs as well as the cell lines of immune system,but not T and B lymphocyte-derived cell lines.The study provides important clues to the basic research of SFTSV.