中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
1期
23-25
,共3页
傅祝能%吴荣辉%孙海芳%王贤芳%孙兰青%陈林海
傅祝能%吳榮輝%孫海芳%王賢芳%孫蘭青%陳林海
부축능%오영휘%손해방%왕현방%손란청%진림해
端粒末端转移酶%宫颈疾病%乳头状瘤病毒,人
耑粒末耑轉移酶%宮頸疾病%乳頭狀瘤病毒,人
단립말단전이매%궁경질병%유두상류병독,인
Telomerase%Cervix diseases%Papillomavirus human%Cervical cancer
目的 检测研究表明女性在感染人乳头瘤病毒(HPV)后引起宫颈炎、宫颈上皮内瘤变(CIN)及进而发展为宫颈癌时其宫颈黏膜脱落细胞端粒酶活性量发生变化,探其对筛查和早期诊治女性宫颈癌患者的临床价值.方法 选HPV检测阴性的正常对照组26例、HPV阳性组28例、HPV阳性宫颈炎组27例、HPV阳性CIN组26例、宫颈癌组24例、HPV阴性宫颈炎组26例、HPV阴性CIN组23例共七个检测组,采集宫颈黏膜脱落细胞标本以293细胞为阳性标准进行端粒酶TRAP实时荧光定量检测.结果 端粒酶活性定量值:正常对照组,范围0～3.15、平均0.36；HPV阳性组,范围0～21.6、平均1.98,＞10的3例；HPV阳性宫颈炎组,范围0～71.5、平均4.88,＞10的4例；HPV阳性CIN组,范围0～ 407、平均20.8,＞10的5例；宫颈癌组,范围1.2 ～5.5×106、中位数346,其中＜10的4例；HPV阴性宫颈炎组,范围0～66.6、平均4.19,＞10的3例；HPV阴性CIN组,范围0 ～158、平均14.2.统计学分析宫颈癌组与其他各组比较差异有统计学意义(P＜0.01),HPV阳性CIN组与正常对照组比较有统计学意义(P＜0.05),其余各组间两两比较无统计学意义(P＞0.05).结论 女性在感染HPV并引起宫颈炎、CIN阶段,其宫颈黏膜脱落细胞端粒酶活性逐步增高,在宫颈癌阶段增高最为显著,因此对采取宫颈黏膜脱落细胞进行端粒酶活性定量检测,可作为对女性宫颈癌的有效筛查和早期诊断手段之一.
目的 檢測研究錶明女性在感染人乳頭瘤病毒(HPV)後引起宮頸炎、宮頸上皮內瘤變(CIN)及進而髮展為宮頸癌時其宮頸黏膜脫落細胞耑粒酶活性量髮生變化,探其對篩查和早期診治女性宮頸癌患者的臨床價值.方法 選HPV檢測陰性的正常對照組26例、HPV暘性組28例、HPV暘性宮頸炎組27例、HPV暘性CIN組26例、宮頸癌組24例、HPV陰性宮頸炎組26例、HPV陰性CIN組23例共七箇檢測組,採集宮頸黏膜脫落細胞標本以293細胞為暘性標準進行耑粒酶TRAP實時熒光定量檢測.結果 耑粒酶活性定量值:正常對照組,範圍0～3.15、平均0.36；HPV暘性組,範圍0～21.6、平均1.98,＞10的3例；HPV暘性宮頸炎組,範圍0～71.5、平均4.88,＞10的4例；HPV暘性CIN組,範圍0～ 407、平均20.8,＞10的5例；宮頸癌組,範圍1.2 ～5.5×106、中位數346,其中＜10的4例；HPV陰性宮頸炎組,範圍0～66.6、平均4.19,＞10的3例；HPV陰性CIN組,範圍0 ～158、平均14.2.統計學分析宮頸癌組與其他各組比較差異有統計學意義(P＜0.01),HPV暘性CIN組與正常對照組比較有統計學意義(P＜0.05),其餘各組間兩兩比較無統計學意義(P＞0.05).結論 女性在感染HPV併引起宮頸炎、CIN階段,其宮頸黏膜脫落細胞耑粒酶活性逐步增高,在宮頸癌階段增高最為顯著,因此對採取宮頸黏膜脫落細胞進行耑粒酶活性定量檢測,可作為對女性宮頸癌的有效篩查和早期診斷手段之一.
목적 검측연구표명녀성재감염인유두류병독(HPV)후인기궁경염、궁경상피내류변(CIN)급진이발전위궁경암시기궁경점막탈락세포단립매활성량발생변화,탐기대사사화조기진치녀성궁경암환자적림상개치.방법 선HPV검측음성적정상대조조26례、HPV양성조28례、HPV양성궁경염조27례、HPV양성CIN조26례、궁경암조24례、HPV음성궁경염조26례、HPV음성CIN조23례공칠개검측조,채집궁경점막탈락세포표본이293세포위양성표준진행단립매TRAP실시형광정량검측.결과 단립매활성정량치:정상대조조,범위0～3.15、평균0.36；HPV양성조,범위0～21.6、평균1.98,＞10적3례；HPV양성궁경염조,범위0～71.5、평균4.88,＞10적4례；HPV양성CIN조,범위0～ 407、평균20.8,＞10적5례；궁경암조,범위1.2 ～5.5×106、중위수346,기중＜10적4례；HPV음성궁경염조,범위0～66.6、평균4.19,＞10적3례；HPV음성CIN조,범위0 ～158、평균14.2.통계학분석궁경암조여기타각조비교차이유통계학의의(P＜0.01),HPV양성CIN조여정상대조조비교유통계학의의(P＜0.05),기여각조간량량비교무통계학의의(P＞0.05).결론 녀성재감염HPV병인기궁경염、CIN계단,기궁경점막탈락세포단립매활성축보증고,재궁경암계단증고최위현저,인차대채취궁경점막탈락세포진행단립매활성정량검측,가작위대녀성궁경암적유효사사화조기진단수단지일.
Objective Studies show that women infected with human papillomavirus (HPV)induced cervicitis,cervical intraepithelial neoplasia (CIN) and on to develop cervical cancer their cervical mucosal exfoliated cells telomerase activity changes,to explore its clinical value in screening and early diagnosis and treatment of female patients with cervical cancer.Methods Elections of HPV testing negative control group 26 cases,HPV-positive group 28 cases,HPV-positive cervicitis group of 27 cases,HPV-positive CIN group 26 cases,24 cases of cervical cancer,HPV-negative cervicitis group 26 cases,HPV-negative CIN group of 23 cases of seven test group,cervical mucosa cytology specimens collected in 293 cells as positive standards for real-time fluorescence quantitative detection of telomerase TRAP Quantitative value of telomerase activity.Results Normal control group,ranging from 0 to 3.15,average 0.36; HPV-positive group,ranging from 0 to 21.6,with an average 1.98,＞ 10,3 cases; HPV-positive cervicitis group,ranging from 0 to 71.5,with an average 4.88,＞ 10,4 cases; HPV-positive CIN group,ranging from0 to 407,with an average 20.8,＞ 10,5 cases; cancer group,the range 1.2-5.5 × 106,median 346,where ＜ 10.4 cases; HPV-negative cervicitis group,ranging from 0 to 66.6,with an average 4.19,＞ 10,3 cases; HPV-negative CIN group,ranging from 0 to 158,with an average 14.2.Statistical analysis of cervical cancer group and the other groups are statistically significant (P ＜0.01),HPV-positive CIN group compared with the control group was statistically significant (P ＜ 0.05) the rest of the comparison between two groups was not statistically significance (P ＞ 0.05).Conclusions Women infected with HPV and cause cervicitis,CIN stage,the cervical mucosa telomerase activity gradually increased and in cervical cancer stage increased the most significant.And therefore take cells exfoliated cervical mucosa quantitative detection of telomerase activity can be used as effective method in screening and early diagnosis for cervical cancer in women.
