中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
1期
28-31
,共4页
崔换弟%金玉%谢广成%程卫霞%章青%段招军
崔換弟%金玉%謝廣成%程衛霞%章青%段招軍
최환제%금옥%사엄성%정위하%장청%단초군
博卡病毒,人%种系发生%重组,遗传
博卡病毒,人%種繫髮生%重組,遺傳
박잡병독,인%충계발생%중조,유전
Bocavirus,human%Phylogeny%Recombination,genetic
目的 扩增人博卡病毒1型的近似全基因组序列,分析HBoV1-4之间的基因重组关系.方法 以检出HBoV1单阳性的鼻咽抽吸物为原始标本,采用PCR的方法扩增HBoV1的5个片段并测序,Blast比对确定是HBoV1片段后用DNAMAN进行序列拼接,拼接序列与已提交的近似全基因组的HBoV1在不同编码区进行同源性分析,并构建进化树；对HBoV1-4的不同编码区进行序列比对和进化分析,揭示HBoV1-4间的基因重组.结果 得到近似全长为5287 bp的HBoV1-NC序列,同源性分析发现HBoV1-NC与重庆株进化关系最近,与广州株进化距离最远；HBoV3可能由HBoV1和HBoV4重组进化而来,HBoV4可能由HBoV2与HBoV3重组进化而来.结论 本研究成功获得近似全基因组的HBoV-NC,HBoV1-4间存在着基因重组关系.
目的 擴增人博卡病毒1型的近似全基因組序列,分析HBoV1-4之間的基因重組關繫.方法 以檢齣HBoV1單暘性的鼻嚥抽吸物為原始標本,採用PCR的方法擴增HBoV1的5箇片段併測序,Blast比對確定是HBoV1片段後用DNAMAN進行序列拼接,拼接序列與已提交的近似全基因組的HBoV1在不同編碼區進行同源性分析,併構建進化樹；對HBoV1-4的不同編碼區進行序列比對和進化分析,揭示HBoV1-4間的基因重組.結果 得到近似全長為5287 bp的HBoV1-NC序列,同源性分析髮現HBoV1-NC與重慶株進化關繫最近,與廣州株進化距離最遠；HBoV3可能由HBoV1和HBoV4重組進化而來,HBoV4可能由HBoV2與HBoV3重組進化而來.結論 本研究成功穫得近似全基因組的HBoV-NC,HBoV1-4間存在著基因重組關繫.
목적 확증인박잡병독1형적근사전기인조서렬,분석HBoV1-4지간적기인중조관계.방법 이검출HBoV1단양성적비인추흡물위원시표본,채용PCR적방법확증HBoV1적5개편단병측서,Blast비대학정시HBoV1편단후용DNAMAN진행서렬병접,병접서렬여이제교적근사전기인조적HBoV1재불동편마구진행동원성분석,병구건진화수；대HBoV1-4적불동편마구진행서렬비대화진화분석,게시HBoV1-4간적기인중조.결과 득도근사전장위5287 bp적HBoV1-NC서렬,동원성분석발현HBoV1-NC여중경주진화관계최근,여엄주주진화거리최원；HBoV3가능유HBoV1화HBoV4중조진화이래,HBoV4가능유HBoV2여HBoV3중조진화이래.결론 본연구성공획득근사전기인조적HBoV-NC,HBoV1-4간존재착기인중조관계.
Objective To amplify the nearly-complete sequence of human Bocavirus 1 (HBoV1)and analyze the recombination relationship among HBoV1-4.Methods Five fragments of HBoV1 genome were amplified by PCR from the HBoV1 single positive nasopharyngeal aspirates,then the five fragments were sequenced and analyzed through the blast in nucleotide acid database.The sequence assembly was conducted by DNAMAN,the homogeneous analysis was performed among the HBoV1 sequences that have been published in nucleotide acid database and then created the phylogenetic tree.At last,the recombination relationship was analyzed among HBoV1-4.Results A total 5287 bp length sequence was amplified in this study,which was the nearly-complete sequence of HBoV1 that was named HBoV1-NC.HBoV1-NC had the closest relationship with Chongqing strain,however,had the farthest relationship with Guangzhou strain through the homogeneous analysis.HBoV3 may be a recombinant derived from HBoV1 and HBoV4,HBoV4 may be a recombinant derived from HBoV2 and HBoV3 through recombination analysis of HBoV1-4.Conclusion The nearly-complete sequence HBoV1-NC was amplified in this study and HBoV1-4 had the recombination relationship.
