CAJ | 학술논문

目的了解近三年北京市朝阳区流行的麻疹野病毒分子流行特征,为本地区消除麻疹提供科学依据.方法采集北京市朝阳区2010-2012年麻疹疑似病例的咽拭子和(或)尿液标本接种vero/SLAM细胞,对分离的麻疹病毒用逆转录及聚合酶链反应扩增麻疹病毒核蛋白(N)基因羧基末端676个核苷酸片段,对扩增产物进行核苷酸序列测定和分析,并以羧基末端450个核苷酸片段构建基因亲缘关系树,进行遗传距离及核苷酸、氨基酸同源性分析.结果分离出18株麻疹病毒,均为H1基因型,H1a亚型.这18株野毒株之间核苷酸同源性为98.2％～100.0％(核苷酸差异为0～8bp),氨基酸变异范围为0～2.7％(氨基酸差异为0～4AA).与H1a亚型参考毒株Chin9322相比,核苷酸变异范围为0.7％～1.6％(核苷酸差异为3 ～7 bp).18株野毒株共有7种基因序列,其中2010年5种、2011年1种、2012年3种基因序列.V2基因序列出现在2010和2012年的病例中,V7序列出现在2010和2011年的病例中.其余5种序列只在单一年份的病例中检出.结论北京近年来流行的本土麻疹病毒为H1a亚型,当年相同基因序列的一组病例提示有麻疹传播链的存在.北京市朝阳区存在基因序列完全相同的麻疹野毒株在2010、2012年循环出现.
목적 료해근삼년북경시조양구류행적마진야병독분자류행특정,위본지구소제마진제공과학의거.방법 채집북경시조양구2010-2012년마진의사병례적인식자화(혹)뇨액표본접충vero/SLAM세포,대분리적마진병독용역전록급취합매련반응확증마진병독핵단백(N)기인최기말단676개핵감산편단,대확증산물진행핵감산서렬측정화분석,병이최기말단450개핵감산편단구건기인친연관계수,진행유전거리급핵감산、안기산동원성분석.결과 분리출18주마진병독,균위H1기인형,H1a아형.저18주야독주지간핵감산동원성위98.2％～100.0％(핵감산차이위0～8bp),안기산변이범위위0～2.7％(안기산차이위0～4AA).여H1a아형삼고독주Chin9322상비,핵감산변이범위위0.7％～1.6％(핵감산차이위3 ～7 bp).18주야독주공유7충기인서렬,기중2010년5충、2011년1충、2012년3충기인서렬.V2기인서렬출현재2010화2012년적병례중,V7서렬출현재2010화2011년적병례중.기여5충서렬지재단일년빈적병례중검출.결론 북경근년래류행적본토마진병독위H1a아형,당년상동기인서렬적일조병례제시유마진전파련적존재.북경시조양구존재기인서렬완전상동적마진야독주재2010、2012년순배출현.
Objective To understand the molecular epidemiology characters of measles wild virus in last three years in Chaoyang District of Beijing,and provide the scientific evidenceformeasles elimination in this region.Methods Vero/Slam cell were used for measlesviral isolationfrom the specimens collected from suspected measles cases in Chaoyang District of Beijing from 2010 to 2012,Fragment of 676 nucleotide acids of the carboxylend of nucleoprotein gene were amplified using reverse transcription-polymerase chain reaction(RT-PCR) method,and then the PCR products were directly sequenced and analyzed.In the end,phylogenetic tree was constructed based on 450 nucleotide acids of the carboxyl end of nucleoprotein gene,and homological analysis was performed at nucleotide acid level and amino acid level.Results 18 measles virus strains were all identified as H1 genotype,and H1 a Sub-genotype.The homology at nucleotide acid level among them was between 98.2％ and 100％ (0-8 nucleotide variation),the homology at amino acid level among them was between 95.3％ and 100％ (0-4 amino acid variation).Compared with Chin9322 the H1aSub-genotype reference strain,the variation at nucleotide acid level of 18 representative measles virus from Chaoyang District in Beijing was between 0.7％-1.6％ (3-7 nucleotide variation).There were 7 nucleotide sequence types,V1 to V7,in the 18 measles virus strains,5 types in 2010,1 type in 2011 and 3 types in 2012.V2 occurred in 2010 and 2012,V7 occurred in 2010 and 2011,other five types justoccurred in single year.Conclusions H 1a sub-genotype was the endemic genotype of wild measles virus circulated in Chaoyang District in Beijing during 2010 to 2012.One sequence type in a cluster of cases indicate that the transmission chains might exist.The measles wild virus with same nucleotide sequence occurred in 2010 and 2012.