中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
2期
114-116
,共3页
彭宏君%侯学伶%李祥%张莉%邓海峰%李敏%史梅%姜庆波%史伟峰
彭宏君%侯學伶%李祥%張莉%鄧海峰%李敏%史梅%薑慶波%史偉峰
팽굉군%후학령%리상%장리%산해봉%리민%사매%강경파%사위봉
肠道病毒属%树突细胞%聚合酶链反应%细胞因子类
腸道病毒屬%樹突細胞%聚閤酶鏈反應%細胞因子類
장도병독속%수돌세포%취합매련반응%세포인자류
Enterovirus%Dendritic cells%Polomerase chain reaction%Cytokines
目的 研究肠道病毒71型(EV71)体外感染人树突状细胞(DCs)后启动免疫应答反应的机制,为探讨EV71感染及宿主免疫应答提供理论依据.方法 健康成人外周血单核细胞诱导衍生获得DCs,流式细胞术检测病毒感染前后DCs表面分子,PCR芯片分析DCs感染EV71 2 h和8h后CD分子及细胞因子类基因差异表达,Luminex液相芯片技术检测感染组细胞上清液中细胞因子含量,混合淋巴细胞反应(MLR)检测病毒感染后的DCs对初始T细胞的刺激活性.结果 EV71感染刺激后DCs的CD80和CD83表达率分别提高了8.2％和35.4％.PCR芯片显示EV71感染2h和8h后CD80、CD83、IL-1α、IL-6和ICAM-1基因表达显著上调2.08～5.49倍,而IL-18则下调5.12倍.与对照组相比,EV71感染DCs组细胞上清液中IL-6和IL-12的含量明显升高(P＜0.05).而MLR未观察到EV71感染的DCs刺激初始T细胞增殖现象.结论 EV71感染能增加DCs活力,延长其存活时间,促使其活化、成熟并分泌细胞因子,启动宿主细胞相应的免疫应答.
目的 研究腸道病毒71型(EV71)體外感染人樹突狀細胞(DCs)後啟動免疫應答反應的機製,為探討EV71感染及宿主免疫應答提供理論依據.方法 健康成人外週血單覈細胞誘導衍生穫得DCs,流式細胞術檢測病毒感染前後DCs錶麵分子,PCR芯片分析DCs感染EV71 2 h和8h後CD分子及細胞因子類基因差異錶達,Luminex液相芯片技術檢測感染組細胞上清液中細胞因子含量,混閤淋巴細胞反應(MLR)檢測病毒感染後的DCs對初始T細胞的刺激活性.結果 EV71感染刺激後DCs的CD80和CD83錶達率分彆提高瞭8.2％和35.4％.PCR芯片顯示EV71感染2h和8h後CD80、CD83、IL-1α、IL-6和ICAM-1基因錶達顯著上調2.08～5.49倍,而IL-18則下調5.12倍.與對照組相比,EV71感染DCs組細胞上清液中IL-6和IL-12的含量明顯升高(P＜0.05).而MLR未觀察到EV71感染的DCs刺激初始T細胞增殖現象.結論 EV71感染能增加DCs活力,延長其存活時間,促使其活化、成熟併分泌細胞因子,啟動宿主細胞相應的免疫應答.
목적 연구장도병독71형(EV71)체외감염인수돌상세포(DCs)후계동면역응답반응적궤제,위탐토EV71감염급숙주면역응답제공이론의거.방법 건강성인외주혈단핵세포유도연생획득DCs,류식세포술검측병독감염전후DCs표면분자,PCR심편분석DCs감염EV71 2 h화8h후CD분자급세포인자류기인차이표체,Luminex액상심편기술검측감염조세포상청액중세포인자함량,혼합림파세포반응(MLR)검측병독감염후적DCs대초시T세포적자격활성.결과 EV71감염자격후DCs적CD80화CD83표체솔분별제고료8.2％화35.4％.PCR심편현시EV71감염2h화8h후CD80、CD83、IL-1α、IL-6화ICAM-1기인표체현저상조2.08～5.49배,이IL-18칙하조5.12배.여대조조상비,EV71감염DCs조세포상청액중IL-6화IL-12적함량명현승고(P＜0.05).이MLR미관찰도EV71감염적DCs자격초시T세포증식현상.결론 EV71감염능증가DCs활력,연장기존활시간,촉사기활화、성숙병분비세포인자,계동숙주세포상응적면역응답.
Objective To study the mechanism of immune responses in enterovirus 71 (EV71)-infected human dendritic cells (DCs) in vitro and provide theoretical basis for EV71 infection and host immune response.Methods Peripheral blood mononuclear cells were purified from the healthy adult peripheral blood and immature DCs were generated from monocytes by culturing in medium containing cytokines.DCs surface markers wereanalyzed by flow cytometry before and after EV71 infection.PCR array was employed to detect the gene expressions of CD molecules and cytokines from EV71-infected DCs at 2 and 8 h postinfection,respectively.The cytokines of EV71-infected DCs in culture supernatants were measured by Luminex liquichip.In addition,the activity of navie T-cell by EV71-infected DCs was examined by mixed-lymphocyte reaction.Results EV71 infection increased the expressing percentages of CD80 and CD83 on DCs by 8.2％ and 35.4％,respectively.PCR array revealed that the expressions of CD80,CD83,IL-1α,IL-6,and ICAM-1 genes were significantly upregulated 2.08 to 5.49-fold at 2 h and 8 h postinfection,while IL-18 gene was downregulated 5.12-fold.Compared with control,the levels of IL-6 and IL-12 were distinctly elevated in EV71-infected DCs (P ＜0.05).However,the proliferation of navie T-cell stimulated by EV71-infected DCs weren't observed by MLR.Conclusion It indicates that EV71 infection not only can increase the viability,the survival time and activation of DCs,but also can significantly increase releases of IL-6 and IL-12 in DCs,which initiate immune response in host cells.
