中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
2期
120-122
,共3页
李红育%张慧%姜中毅%李保娣%于德山
李紅育%張慧%薑中毅%李保娣%于德山
리홍육%장혜%강중의%리보제%우덕산
流感病毒A型%流感病毒B型%免疫学监测%聚合酶链反应%病毒培养
流感病毒A型%流感病毒B型%免疫學鑑測%聚閤酶鏈反應%病毒培養
류감병독A형%류감병독B형%면역학감측%취합매련반응%병독배양
Influenza A virus%Influenza B virus%Immundogic surveillance%Polyomerage chain reaclion%Virus cultirations
目的 对2009-2012年甘肃省流感监测结果进行分析,为甘肃省的流感防控工作提供科学依据.方法 全省19家国家级流感监测哨点医院采集流感样病例的咽拭子,各地医疗机构送检流感样暴发疫情标本,以荧光定量PCR方法检测病毒核酸,用MDCK进行病毒分离培养,送中国疾病预防控制中心病毒病所进行复核及序列分析.结果 共采集流感样病例咽拭子36 892份,检测出流感核酸阳性8030例,检出率为21.77％,其中新甲型H1N1 4331份,季节性H3 1562份,季节性H196份,B型1651份,A未分型380份.对7073份标本进行病毒分离,分离到病毒1149株,其中新甲型H1N1 346株,季节性H3 183株,季节性H1 34株,B型Victorian系470株,Yamagata系116株.按年度分析,2009-2012年流感病毒检出率依次为32.71％、13.95％、7.80％和19.64％;按月分析,在10-12月份A型流感病毒阳性率及构成比均高于其他月份,阳性率平均为31.33％,构成比为97.61％;2-4月份B型流感病毒阳性率及构成比分别为16.51％和81.10％;5-9月份为流感病毒阳性率仅为5.03％(2009年不统计).按年龄组分析,＜1岁婴儿检出率最低为7.21％,显著低于与其他年龄组;10～20岁最高为34.26％.结论 甘肃省2009年以新甲型H1N1流感流行为主,2010-2011年流感活动很弱,仅为散发,2012年春季以B型为主,11月开始以甲型H1N1及季节性H3为主.流感流行每年均有一个高峰,病毒型别表现为交替流行的规律,甲型流感是冬季儿童常见的传染病.
目的 對2009-2012年甘肅省流感鑑測結果進行分析,為甘肅省的流感防控工作提供科學依據.方法 全省19傢國傢級流感鑑測哨點醫院採集流感樣病例的嚥拭子,各地醫療機構送檢流感樣暴髮疫情標本,以熒光定量PCR方法檢測病毒覈痠,用MDCK進行病毒分離培養,送中國疾病預防控製中心病毒病所進行複覈及序列分析.結果 共採集流感樣病例嚥拭子36 892份,檢測齣流感覈痠暘性8030例,檢齣率為21.77％,其中新甲型H1N1 4331份,季節性H3 1562份,季節性H196份,B型1651份,A未分型380份.對7073份標本進行病毒分離,分離到病毒1149株,其中新甲型H1N1 346株,季節性H3 183株,季節性H1 34株,B型Victorian繫470株,Yamagata繫116株.按年度分析,2009-2012年流感病毒檢齣率依次為32.71％、13.95％、7.80％和19.64％;按月分析,在10-12月份A型流感病毒暘性率及構成比均高于其他月份,暘性率平均為31.33％,構成比為97.61％;2-4月份B型流感病毒暘性率及構成比分彆為16.51％和81.10％;5-9月份為流感病毒暘性率僅為5.03％(2009年不統計).按年齡組分析,＜1歲嬰兒檢齣率最低為7.21％,顯著低于與其他年齡組;10～20歲最高為34.26％.結論 甘肅省2009年以新甲型H1N1流感流行為主,2010-2011年流感活動很弱,僅為散髮,2012年春季以B型為主,11月開始以甲型H1N1及季節性H3為主.流感流行每年均有一箇高峰,病毒型彆錶現為交替流行的規律,甲型流感是鼕季兒童常見的傳染病.
목적 대2009-2012년감숙성류감감측결과진행분석,위감숙성적류감방공공작제공과학의거.방법 전성19가국가급류감감측초점의원채집류감양병례적인식자,각지의료궤구송검류감양폭발역정표본,이형광정량PCR방법검측병독핵산,용MDCK진행병독분리배양,송중국질병예방공제중심병독병소진행복핵급서렬분석.결과 공채집류감양병례인식자36 892빈,검측출류감핵산양성8030례,검출솔위21.77％,기중신갑형H1N1 4331빈,계절성H3 1562빈,계절성H196빈,B형1651빈,A미분형380빈.대7073빈표본진행병독분리,분리도병독1149주,기중신갑형H1N1 346주,계절성H3 183주,계절성H1 34주,B형Victorian계470주,Yamagata계116주.안년도분석,2009-2012년류감병독검출솔의차위32.71％、13.95％、7.80％화19.64％;안월분석,재10-12월빈A형류감병독양성솔급구성비균고우기타월빈,양성솔평균위31.33％,구성비위97.61％;2-4월빈B형류감병독양성솔급구성비분별위16.51％화81.10％;5-9월빈위류감병독양성솔부위5.03％(2009년불통계).안년령조분석,＜1세영인검출솔최저위7.21％,현저저우여기타년령조;10～20세최고위34.26％.결론 감숙성2009년이신갑형H1N1류감류행위주,2010-2011년류감활동흔약,부위산발,2012년춘계이B형위주,11월개시이갑형H1N1급계절성H3위주.류감류행매년균유일개고봉,병독형별표현위교체류행적규률,갑형류감시동계인동상견적전염병.
Objective To study the epidemical characteristics of influenza in Gansu province from 2009 to 2012,and to discuss the changes of the predominant strains of influenza virus.In order to provide scientific basis for defending influenza effectively.Methods Influenza surveillance was performed by collecting the pharyngeal swab specimens from influenza patients of national surveillance hospitals and places with suspected influenza epidemic situations,inoculated on MDCK cell culture to obtain the influenza virus strains.Results From Jan.2009 to Dce.2012 36892 specimens were collected,and 8030 were positive for influenza virus,including 4331 influenza A (H1 N1),seasonal H3N2 subtype1562,H1N1 subtype 96,1651 type B,and 380 type A with unknown subtype strains.During the period from 2009 to 2012,the positive of influenza virus isolation was 32.71％,13.95％,7.80％,and 19.64％,respectively.Totally 1149 influenza virus strains were isolated,positive rate was 16.24％,of them,346 strains were SWA1 (H1pdmN1),34 strains were A1 (H1N1),183 strains were A3 (H3N2),470 strains were subtype B of Victoria and 116 strains were subtype B of Yamagata.Conclusion In 2009 new influenza A (H1N1) was the major epidemic,and in 2010-2011 the activity of influenza was very weak,only presented as sporadic disease.In the spring of 2012,type B influenza was dominant,while since Nov.type A (H1N1) and seasonal H3 were dominant.There was a peak of influenza epidemic each year,and alternative viral types predominance was the rule.
