中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
3期
173-175
,共3页
张宝敏%陈海云%檀晓娟%李静%冀天娇%曾祥洁%祝双利%许文波%马焱
張寶敏%陳海雲%檀曉娟%李靜%冀天嬌%曾祥潔%祝雙利%許文波%馬焱
장보민%진해운%단효연%리정%기천교%증상길%축쌍리%허문파%마염
柯萨奇病毒感染%手足口病%基因型
柯薩奇病毒感染%手足口病%基因型
가살기병독감염%수족구병%기인형
Coxsackievirus infection%Hand-foot-and-mouth disease%Genotype
目的 分析2010年引起海南省手足口病流行柯萨奇病毒A组16型(Coxsackievirus A16,CA16)的分子流行病学特征.方法 从海南省2010年手足口病(hand,foot,and mouth disease,HFMD)标本中分离到56株CA16毒株,用RT-PCR的方法对VP1编码区扩增及核苷酸序列的测定和整理分析,并与CA16各基因型和基因亚型的代表株序列构建亲缘关系进化树.结果 其中34株属于B基因型中的B1a基因亚型,22株属于B1b基因亚型.B1a基因亚型的毒株主要分布于海口市以及海南省西北部相邻的4个县市,B1b基因亚型的毒株主要分布于海口市以及海南省中部、西南部相邻的4个县市.结论 2010年海南省B1a和B1b基因亚型在人群中共循环,两个基因亚型呈现一定区域性分布的特点,提示在海南省CA16至少存在两条大的传播链.在2008年至2010年,海南省手足口病例中CA16感染的比例明显上升,分别为8.05％、5.35％、35.45％.
目的 分析2010年引起海南省手足口病流行柯薩奇病毒A組16型(Coxsackievirus A16,CA16)的分子流行病學特徵.方法 從海南省2010年手足口病(hand,foot,and mouth disease,HFMD)標本中分離到56株CA16毒株,用RT-PCR的方法對VP1編碼區擴增及覈苷痠序列的測定和整理分析,併與CA16各基因型和基因亞型的代錶株序列構建親緣關繫進化樹.結果 其中34株屬于B基因型中的B1a基因亞型,22株屬于B1b基因亞型.B1a基因亞型的毒株主要分佈于海口市以及海南省西北部相鄰的4箇縣市,B1b基因亞型的毒株主要分佈于海口市以及海南省中部、西南部相鄰的4箇縣市.結論 2010年海南省B1a和B1b基因亞型在人群中共循環,兩箇基因亞型呈現一定區域性分佈的特點,提示在海南省CA16至少存在兩條大的傳播鏈.在2008年至2010年,海南省手足口病例中CA16感染的比例明顯上升,分彆為8.05％、5.35％、35.45％.
목적 분석2010년인기해남성수족구병류행가살기병독A조16형(Coxsackievirus A16,CA16)적분자류행병학특정.방법 종해남성2010년수족구병(hand,foot,and mouth disease,HFMD)표본중분리도56주CA16독주,용RT-PCR적방법대VP1편마구확증급핵감산서렬적측정화정리분석,병여CA16각기인형화기인아형적대표주서렬구건친연관계진화수.결과 기중34주속우B기인형중적B1a기인아형,22주속우B1b기인아형.B1a기인아형적독주주요분포우해구시이급해남성서북부상린적4개현시,B1b기인아형적독주주요분포우해구시이급해남성중부、서남부상린적4개현시.결론 2010년해남성B1a화B1b기인아형재인군중공순배,량개기인아형정현일정구역성분포적특점,제시재해남성CA16지소존재량조대적전파련.재2008년지2010년,해남성수족구병례중CA16감염적비례명현상승,분별위8.05％、5.35％、35.45％.
Objective To analyzed the molecular epidemic characteristics of Coxsackievirus A 16 (CA16) which had caused the spread of hand-foot-and-mouth disease (HFMD) in Hainan province during 2010.Methods Totally 56 CA16 strains were isolated from patients with HFMD in Hainan Province during 2010.Reverse transcription polymerase chain reaction (RT-PCR) was used to amplify and sequence the VP1 coding region.These sequences were used together with those of CA16 representative strains to construct the phylogenetic tree.Results And those of 34 strains belonged to genetic subtype B1a of B1 genetic subtype,22 strains belonged to genetic subtype B1b.The B1a genetic subtypes strains were mainly isolated in Haikou City and other four adjacent counties located in the northwest of Hainan Province,and the B1b genetic subtypes strains were mainly isolated in Haikou City and other four adjacent counties located in the middle,southwest of Hainan Province.Conclusion Genetic subtype B1 a and B 1b were prevalentand co-occurring in Hainan during 2010.The distribution of CA16 strains had a certain of geographical characteristics.The results suggested there were at least two large chains of CA16 transmission in Hainan.In 2008-2010,the percentage of CA16 infection in Hainan province from HFMD cases was 8.05％,5.35％,35.45％respectively,which indicates it has increased obviously.
