CAJ | 학술논문

目的分析2010年宁夏回族自治区流行的柯萨奇病毒A组16型(Coxsackievirus A16,CA16)VP1基因特征.方法用特异引物通过逆转录聚合酶链反应(reverse transcriptase polymerase chain reaction,RT-PCR),对2010年分离自宁夏手足口病(hand,foot,and mouth disease,HFMD)病例的CA16毒株,进行VP1编码基因的扩增.扩增阳性产物进行序列测定,采用生物信息学软件进行分析.结果本研究共获得62株CA16的VP1编码序列,其核苷酸和氨基酸序列同源性分别为90.1％～100％(平均94.5％)和98.7％～100％(平均99.6％).在亲缘性关系树上与B1基因亚型毒株位于相同分支,且各个地市流行株又同时存在B1a和B1b分支.结论与全国流行情况相一致,宁夏2010年存在B1a和B1b分支CA16的共循环,毒株间具有较大的核苷酸序列差异,提示流行中存在多个传播链.
목적 분석2010년저하회족자치구류행적가살기병독A조16형(Coxsackievirus A16,CA16)VP1기인특정.방법 용특이인물통과역전록취합매련반응(reverse transcriptase polymerase chain reaction,RT-PCR),대2010년분리자저하수족구병(hand,foot,and mouth disease,HFMD)병례적CA16독주,진행VP1편마기인적확증.확증양성산물진행서렬측정,채용생물신식학연건진행분석.결과 본연구공획득62주CA16적VP1편마서렬,기핵감산화안기산서렬동원성분별위90.1％～100％(평균94.5％)화98.7％～100％(평균99.6％).재친연성관계수상여B1기인아형독주위우상동분지,차각개지시류행주우동시존재B1a화B1b분지.결론 여전국류행정황상일치,저하2010년존재B1a화B1b분지CA16적공순배,독주간구유교대적핵감산서렬차이,제시류행중존재다개전파련.
Objective To reveal the genetic characteristics of VP1 encoding region of Coxsackievirus A16 (CA16) in Ningxia Hui Autonomous Region,2010.Methods With specific primers,reverse transcriptase polymerase chain reaction (RT-PCR) were performed to amplify the complete VP1 encoding sequence from CA16 isolates,which were isolates from hand,foot,and mouth disease (HFMD) patients in Ningxia in 2010.The VP1 sequences were determined,and analyzed with bioinformatic software.Results Totally,VP1 of 62 CA16 strains were determined,showing 90.1％-100％ (average,94.5％) identities of nucleotide sequences and 98.7％-100％ (average,99.6％) identities of amino acid sequences.On phylogenetic tree,all of the viruses in this study clustered together with subgenotype B1,which could be divided into clade B1a and B1b further.And viruses from each prefecture in Ningxia included both clade B1a and clade B1b.Conclusions Consistent with CA16 from other provinces,CA16 circulating in Ningxia in 2010 included both clade B1a and B1b.These viruses showed high diversity of VP1 nucleotide sequences,suggesting heterologous CA16 strains were co-circulating in Ningxia in year 2010.